Generation of autism spectrum disorder patient-derived iPSC line SDUKIi004-A

Morad Kamand; Mirolyuba Ilieva; Sheena Louise Forsberg; Mads Thomassen; Åsa Fex Svenningsen; Morten Meyer; Tanja Maria Michel

doi:10.1016/j.scr.2020.102038

Generation of autism spectrum disorder patient-derived iPSC line SDUKIi004-A

Morad Kamand^*, Mirolyuba Ilieva, Sheena Louise Forsberg, Mads Thomassen, Åsa Fex Svenningsen, Morten Meyer, Tanja Maria Michel

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Autism is a heterogeneous neurodevelopmental disorder defined by deficits in socialization, communication, and patterns of behavior. Using stem cells to model brain disorders may yield new understanding about the underlying neuropathological processes and could prove essential for drug development. We present here a new human induced pluripotent stem cell (iPSC) line (SDUKIi004-A) generated from skin fibroblasts derived from a 21-year old male patient diagnosed with Pervasive Developmental Disorder-Not Otherwise Specified (PDD-NOS) (“FYNEN-cohort”). Reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

Original language	English
Article number	102038
Journal	Stem Cell Research
Volume	49
Number of pages	5
ISSN	1873-5061
DOIs	https://doi.org/10.1016/j.scr.2020.102038
Publication status	Published - Dec 2020

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10.1016/j.scr.2020.102038Licence: CC BY-NC-ND

Open Access VersionFinal published version, 3.34 MBLicence: CC BY-NC-ND

1 Ph.D. thesis

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain
Kamand, M., 24. Jun 2022, Syddansk Universitet. Det Sundhedsvidenskabelige Fakultet. 225 p.
Research output: Thesis › Ph.D. thesis

Cite this

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title = "Generation of autism spectrum disorder patient-derived iPSC line SDUKIi004-A",

abstract = "Autism is a heterogeneous neurodevelopmental disorder defined by deficits in socialization, communication, and patterns of behavior. Using stem cells to model brain disorders may yield new understanding about the underlying neuropathological processes and could prove essential for drug development. We present here a new human induced pluripotent stem cell (iPSC) line (SDUKIi004-A) generated from skin fibroblasts derived from a 21-year old male patient diagnosed with Pervasive Developmental Disorder-Not Otherwise Specified (PDD-NOS) (“FYNEN-cohort”). Reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.",

author = "Morad Kamand and Mirolyuba Ilieva and Forsberg, {Sheena Louise} and Mads Thomassen and Svenningsen, {{\AA}sa Fex} and Morten Meyer and Michel, {Tanja Maria}",

year = "2020",

month = dec,

doi = "10.1016/j.scr.2020.102038",

language = "English",

volume = "49",

journal = "Stem Cell Research",

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AU - Kamand, Morad

AU - Ilieva, Mirolyuba

AU - Forsberg, Sheena Louise

AU - Thomassen, Mads

AU - Svenningsen, Åsa Fex

AU - Meyer, Morten

AU - Michel, Tanja Maria

PY - 2020/12

Y1 - 2020/12

N2 - Autism is a heterogeneous neurodevelopmental disorder defined by deficits in socialization, communication, and patterns of behavior. Using stem cells to model brain disorders may yield new understanding about the underlying neuropathological processes and could prove essential for drug development. We present here a new human induced pluripotent stem cell (iPSC) line (SDUKIi004-A) generated from skin fibroblasts derived from a 21-year old male patient diagnosed with Pervasive Developmental Disorder-Not Otherwise Specified (PDD-NOS) (“FYNEN-cohort”). Reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

AB - Autism is a heterogeneous neurodevelopmental disorder defined by deficits in socialization, communication, and patterns of behavior. Using stem cells to model brain disorders may yield new understanding about the underlying neuropathological processes and could prove essential for drug development. We present here a new human induced pluripotent stem cell (iPSC) line (SDUKIi004-A) generated from skin fibroblasts derived from a 21-year old male patient diagnosed with Pervasive Developmental Disorder-Not Otherwise Specified (PDD-NOS) (“FYNEN-cohort”). Reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

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DO - 10.1016/j.scr.2020.102038

M3 - Journal article

C2 - 33068890

AN - SCOPUS:85092470351

SN - 1873-5061

VL - 49

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102038

ER -

Generation of autism spectrum disorder patient-derived iPSC line SDUKIi004-A

Abstract

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Related research output

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain

Cite this