Galectin-3 is Persistently Increased in Early Rheumatoid Arthritis (RA) and Associates with Anti-CCP Seropositivity and MRI Bone Lesions, While Early Fibrosis Markers Correlate with Disease Activity

Saida Farah Issa, Anne Friesgaard Christensen, Hanne M Lindegaard, Merete L Hetland, Kim Hørslev-Petersen, Kristian Stengaard-Pedersen, Bo Jannik Ejbjerg, Tine Lottenburger, Torkell Ellingsen, Jens Kristian Pedersen, Kirsten Junker, Anders Svendsen, Ulrik Tarp, Mikkel Østergaard, Peter Junker

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Galectin-3 has been suggested as a pro-inflammatory mediator in animal arthritis and rheumatoid arthritis (RA). We aimed to study the serum level of galectin-3 in patients with newly diagnosed RA and associations with disease profile, Magnetic Resonance Imaging (MRI) findings and seromarkers of synovial matrix inflammation. One hundred and sixty DMARD naïve patients newly diagnosed with RA were included (CIMESTRA study). Clinical, serological and imaging data were recorded before treatment and at 6 weeks, 3 and 12 months. Galectin-3 and hyaluronan (HYA) were measured by ELISA (R&D and Corgenix, USA), and the N-terminal propeptide of type III collagen (PIIINP) by radioimmuno assay (Orion Diagnostica, Finland). One-hundred-and-nineteen, 87 and 60 blood donors served as controls for galectin-3, HYA and PIIINP, respectively. Baseline galectin-3 was significantly elevated in anti-CCP positive (4.2 μg/l IQR [3.6;6.1]) patients as compared with anti-CCP negatives (4.0 μg/l [2.6;4.9], p=0.05) and controls (3.8 μg/l [3.0;4.8], p<0.01). During treatment, galectin 3 remained elevated, but increased transiently with peak values at 6 weeks. Galectin-3 correlated with baseline smoking, anti-CCP, and with MRI erosion score after 1 year of follow-up. HYA and PIIINP were elevated (p<0.001) irrespective of anti-CCP status and correlated positively with synovitis assessed clinically and by MRI. HYA and PIIINP did not correlate with galectin-3. These observations indicate that HYA and PIIINP mainly reflect expansive synovitis proliferation while galectin-3 is more closely linked to autoimmunity, smoking and joint destructive processes. This article is protected by copyright. All rights reserved.

Original languageEnglish
JournalScandinavian Journal of Immunology
Volume86
Issue number6
Pages (from-to)471–478
ISSN0300-9475
DOIs
Publication statusPublished - Dec 2017

Keywords

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Anti-Citrullinated Protein Antibodies/blood
  • Arthritis, Rheumatoid/diagnosis
  • Biomarkers/metabolism
  • Bone Resorption
  • Bone and Bones/metabolism
  • Disease Progression
  • Female
  • Fibrosis
  • Follow-Up Studies
  • Galectin 3/metabolism
  • Humans
  • Magnetic Resonance Imaging
  • Male
  • Middle Aged
  • Synovial Membrane/metabolism
  • Young Adult

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