Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices

Tanya Bakmand, Ane R Troels-Smith, Maria Dimaki, Jakob D Nissen, Karsten B Andersen, Luigi Sasso, Helle S Waagepetersen, Jan B Gramsbergen, Winnie E Svendsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.

Original languageEnglish
JournalBiomedical Microdevices
Volume17
Issue number4
Pages (from-to)71
ISSN1387-2176
DOIs
Publication statusPublished - Aug 2015

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Fluidics
Brain
Monitoring
Tissue
Electrophysiology
Equipment and Supplies
Nutrients
Glucose
Food
Computer simulation
Growth
In Vitro Techniques

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Bakmand, T., Troels-Smith, A. R., Dimaki, M., Nissen, J. D., Andersen, K. B., Sasso, L., ... Svendsen, W. E. (2015). Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices. Biomedical Microdevices, 17(4), 71. https://doi.org/10.1007/s10544-015-9973-6
Bakmand, Tanya ; Troels-Smith, Ane R ; Dimaki, Maria ; Nissen, Jakob D ; Andersen, Karsten B ; Sasso, Luigi ; Waagepetersen, Helle S ; Gramsbergen, Jan B ; Svendsen, Winnie E. / Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices. In: Biomedical Microdevices. 2015 ; Vol. 17, No. 4. pp. 71.
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abstract = "Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.",
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Bakmand, T, Troels-Smith, AR, Dimaki, M, Nissen, JD, Andersen, KB, Sasso, L, Waagepetersen, HS, Gramsbergen, JB & Svendsen, WE 2015, 'Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices', Biomedical Microdevices, vol. 17, no. 4, pp. 71. https://doi.org/10.1007/s10544-015-9973-6

Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices. / Bakmand, Tanya; Troels-Smith, Ane R; Dimaki, Maria; Nissen, Jakob D; Andersen, Karsten B; Sasso, Luigi; Waagepetersen, Helle S; Gramsbergen, Jan B; Svendsen, Winnie E.

In: Biomedical Microdevices, Vol. 17, No. 4, 08.2015, p. 71.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices

AU - Bakmand, Tanya

AU - Troels-Smith, Ane R

AU - Dimaki, Maria

AU - Nissen, Jakob D

AU - Andersen, Karsten B

AU - Sasso, Luigi

AU - Waagepetersen, Helle S

AU - Gramsbergen, Jan B

AU - Svendsen, Winnie E

PY - 2015/8

Y1 - 2015/8

N2 - Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.

AB - Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.

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