Flow cytometric detection of growth factor receptors in autografts and analysis of growth factor concentrations in autologous stem cell transplantation: possible significance for platelet recovery.

I Schiødt, Charlotte Harken Jensen, E Kjaersgaard, E Gaarsdal, K Nikolajsen, H E Johnsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In order to improve prediction of hematopoietic recovery, we conducted a pilot study, analyzing the significance of growth factor receptor expression in autografts as well as endogenous growth factor levels in blood before, during and after stem cell transplantation. Three early acting (stem cell factor (SCF), Flt3 ligand (Flt3) and fetal antigen 1 (FA1)) and three lineage-specific growth factors (EPO, G-CSF and thrombopoietin (Tpo)) were analyzed by ELISA in 16 patients with multiple myeloma (MM) and 16 patients with non-Hodgkin's lymphoma (NHL). The relative number of SCF, Flt3, Tpo and G-CSF receptor positive, CD34+ progenitor cells were measured by flow cytometry in the leukapheresis product used for transplantation in a subgroup of 15 patients (NHL, n = 8, MM, n = 7). Three factors were identified as having a significant impact on platelet recovery. First, the level of Tpo in blood at the time of the nadir (day +7). Second, the percentage of re-infused thrombopoietin receptor positive progenitors and finally, the percentage of Flt3 receptor positive progenitors. On the other hand, none of the analyzed factors significantly predicted myeloid or erythroid recovery. These findings need to be confirmed in prospectively designed studies.
Udgivelsesdato: 2000-Sep
Original languageEnglish
JournalBone Marrow Transplantation
Volume26
Issue number5
Pages (from-to)525-31
Number of pages6
ISSN0268-3369
DOIs
Publication statusPublished - 1 Sep 2000

Keywords

  • Adjuvants, Immunologic
  • Adult
  • Aged
  • Antigens, CD34
  • Biological Markers
  • Female
  • Flow Cytometry
  • Glycoproteins
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells
  • Humans
  • Lymphoma, Non-Hodgkin
  • Membrane Proteins
  • Middle Aged
  • Multiple Myeloma
  • Neoplasm Proteins
  • Pilot Projects
  • Platelet Count
  • Prognosis
  • Prospective Studies
  • Proto-Oncogene Proteins
  • Receptors, Cytokine
  • Receptors, Erythropoietin
  • Receptors, Granulocyte Colony-Stimulating Factor
  • Receptors, Growth Factor
  • Receptors, Thrombopoietin
  • Transplantation, Autologous

Cite this

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title = "Flow cytometric detection of growth factor receptors in autografts and analysis of growth factor concentrations in autologous stem cell transplantation: possible significance for platelet recovery.",
abstract = "In order to improve prediction of hematopoietic recovery, we conducted a pilot study, analyzing the significance of growth factor receptor expression in autografts as well as endogenous growth factor levels in blood before, during and after stem cell transplantation. Three early acting (stem cell factor (SCF), Flt3 ligand (Flt3) and fetal antigen 1 (FA1)) and three lineage-specific growth factors (EPO, G-CSF and thrombopoietin (Tpo)) were analyzed by ELISA in 16 patients with multiple myeloma (MM) and 16 patients with non-Hodgkin's lymphoma (NHL). The relative number of SCF, Flt3, Tpo and G-CSF receptor positive, CD34+ progenitor cells were measured by flow cytometry in the leukapheresis product used for transplantation in a subgroup of 15 patients (NHL, n = 8, MM, n = 7). Three factors were identified as having a significant impact on platelet recovery. First, the level of Tpo in blood at the time of the nadir (day +7). Second, the percentage of re-infused thrombopoietin receptor positive progenitors and finally, the percentage of Flt3 receptor positive progenitors. On the other hand, none of the analyzed factors significantly predicted myeloid or erythroid recovery. These findings need to be confirmed in prospectively designed studies. Udgivelsesdato: 2000-Sep",
keywords = "Adjuvants, Immunologic, Adult, Aged, Antigens, CD34, Biological Markers, Female, Flow Cytometry, Glycoproteins, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells, Humans, Lymphoma, Non-Hodgkin, Membrane Proteins, Middle Aged, Multiple Myeloma, Neoplasm Proteins, Pilot Projects, Platelet Count, Prognosis, Prospective Studies, Proto-Oncogene Proteins, Receptors, Cytokine, Receptors, Erythropoietin, Receptors, Granulocyte Colony-Stimulating Factor, Receptors, Growth Factor, Receptors, Thrombopoietin, Transplantation, Autologous",
author = "I Schi{\o}dt and Jensen, {Charlotte Harken} and E Kjaersgaard and E Gaarsdal and K Nikolajsen and Johnsen, {H E}",
year = "2000",
month = "9",
day = "1",
doi = "10.1038/sj.bmt.1702554",
language = "English",
volume = "26",
pages = "525--31",
journal = "Bone Marrow Transplantation",
issn = "0268-3369",
publisher = "Nature Publishing Group",
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Flow cytometric detection of growth factor receptors in autografts and analysis of growth factor concentrations in autologous stem cell transplantation: possible significance for platelet recovery. / Schiødt, I; Jensen, Charlotte Harken; Kjaersgaard, E; Gaarsdal, E; Nikolajsen, K; Johnsen, H E.

In: Bone Marrow Transplantation, Vol. 26, No. 5, 01.09.2000, p. 525-31.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Flow cytometric detection of growth factor receptors in autografts and analysis of growth factor concentrations in autologous stem cell transplantation: possible significance for platelet recovery.

AU - Schiødt, I

AU - Jensen, Charlotte Harken

AU - Kjaersgaard, E

AU - Gaarsdal, E

AU - Nikolajsen, K

AU - Johnsen, H E

PY - 2000/9/1

Y1 - 2000/9/1

N2 - In order to improve prediction of hematopoietic recovery, we conducted a pilot study, analyzing the significance of growth factor receptor expression in autografts as well as endogenous growth factor levels in blood before, during and after stem cell transplantation. Three early acting (stem cell factor (SCF), Flt3 ligand (Flt3) and fetal antigen 1 (FA1)) and three lineage-specific growth factors (EPO, G-CSF and thrombopoietin (Tpo)) were analyzed by ELISA in 16 patients with multiple myeloma (MM) and 16 patients with non-Hodgkin's lymphoma (NHL). The relative number of SCF, Flt3, Tpo and G-CSF receptor positive, CD34+ progenitor cells were measured by flow cytometry in the leukapheresis product used for transplantation in a subgroup of 15 patients (NHL, n = 8, MM, n = 7). Three factors were identified as having a significant impact on platelet recovery. First, the level of Tpo in blood at the time of the nadir (day +7). Second, the percentage of re-infused thrombopoietin receptor positive progenitors and finally, the percentage of Flt3 receptor positive progenitors. On the other hand, none of the analyzed factors significantly predicted myeloid or erythroid recovery. These findings need to be confirmed in prospectively designed studies. Udgivelsesdato: 2000-Sep

AB - In order to improve prediction of hematopoietic recovery, we conducted a pilot study, analyzing the significance of growth factor receptor expression in autografts as well as endogenous growth factor levels in blood before, during and after stem cell transplantation. Three early acting (stem cell factor (SCF), Flt3 ligand (Flt3) and fetal antigen 1 (FA1)) and three lineage-specific growth factors (EPO, G-CSF and thrombopoietin (Tpo)) were analyzed by ELISA in 16 patients with multiple myeloma (MM) and 16 patients with non-Hodgkin's lymphoma (NHL). The relative number of SCF, Flt3, Tpo and G-CSF receptor positive, CD34+ progenitor cells were measured by flow cytometry in the leukapheresis product used for transplantation in a subgroup of 15 patients (NHL, n = 8, MM, n = 7). Three factors were identified as having a significant impact on platelet recovery. First, the level of Tpo in blood at the time of the nadir (day +7). Second, the percentage of re-infused thrombopoietin receptor positive progenitors and finally, the percentage of Flt3 receptor positive progenitors. On the other hand, none of the analyzed factors significantly predicted myeloid or erythroid recovery. These findings need to be confirmed in prospectively designed studies. Udgivelsesdato: 2000-Sep

KW - Adjuvants, Immunologic

KW - Adult

KW - Aged

KW - Antigens, CD34

KW - Biological Markers

KW - Female

KW - Flow Cytometry

KW - Glycoproteins

KW - Hematopoietic Stem Cell Transplantation

KW - Hematopoietic Stem Cells

KW - Humans

KW - Lymphoma, Non-Hodgkin

KW - Membrane Proteins

KW - Middle Aged

KW - Multiple Myeloma

KW - Neoplasm Proteins

KW - Pilot Projects

KW - Platelet Count

KW - Prognosis

KW - Prospective Studies

KW - Proto-Oncogene Proteins

KW - Receptors, Cytokine

KW - Receptors, Erythropoietin

KW - Receptors, Granulocyte Colony-Stimulating Factor

KW - Receptors, Growth Factor

KW - Receptors, Thrombopoietin

KW - Transplantation, Autologous

U2 - 10.1038/sj.bmt.1702554

DO - 10.1038/sj.bmt.1702554

M3 - Journal article

VL - 26

SP - 525

EP - 531

JO - Bone Marrow Transplantation

JF - Bone Marrow Transplantation

SN - 0268-3369

IS - 5

ER -