Field samplings of Ixodes ricinus ticks from a tick-borne encephalitis virus micro-focus in Northern Zealand, Denmark

Andreas Petersen; Maiken Worsøe Rosenstierne; Morten Rasmussen; Kurt Fuursted; Henrik Vedel Nielsen; Lee O'Brien Andersen; René Bødker; Anders Fomsgaard

doi:10.1016/j.ttbdis.2019.05.005

Field samplings of Ixodes ricinus ticks from a tick-borne encephalitis virus micro-focus in Northern Zealand, Denmark

Andreas Petersen^*
, Maiken Worsøe Rosenstierne
, Morten Rasmussen
, Kurt Fuursted
, Henrik Vedel Nielsen
, Lee O'Brien Andersen
, René Bødker
, Anders Fomsgaard

^*Corresponding author for this work

KI, OUH, Research unit of Infectious Diseases (Odense)

European Center for Disease Prevention and Control
Statens Serum Institut
Technical University of Denmark

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

In 2008–2009 a tick-borne encephalitis virus (TBEV)micro-focus was detected in Northern Zealand, Denmark. No new cases of TBE with an epidemiological link to Northern Zealand has been reported since. Here we undertook to investigate Ixodes ricinus ticks from this endemic micro-focus in 2016 and 2017. In addition to TBEV, I. ricinus ticks may host other pathogens that include Borrelia spp., Babesia spp., Rickettsia spp. and Neoehrlichia mikurensis, together with various endosymbiont microorganisms. To detect multiple organisms we used a metagenomics PanVirus microarray and next-generation sequencing to examine the persistence and evolution of other emerging viruses, bacteria and parasites. Here we report the rise and fall of the Danish TBEV micro-focus in Northern Zealand. However, we identify for the first time in Danish I. ricinus ticks the presence of Uukuniemi virus in addition to a tick-borne phlebovirus and a range of bacteria.

Original language	English
Journal	Ticks and Tick-borne Diseases
Volume	10
Issue number	5
Pages (from-to)	1028-1032
ISSN	1877-959X
DOIs	https://doi.org/10.1016/j.ttbdis.2019.05.005
Publication status	Published - 1. Aug 2019

Funding

We thank Susanne Lopez Rasmussen, Louise Borup, Randi Thøgersen for technical assistance and Maria Magdalena Lassaunière for editorial assistance. This study is part of the “Metagenomic Array Detection of emerging Virus in EU” (MAD-Vir) project, which is part of the European Joint Programme One Health EJP consortium, which has received funding from the European Union’s Horizon 2020 research and innovation programme under Grant Agreement No 773830 . Appendix A

Keywords

Bacteria
Ixodes ricinus
Micro-focus
Tick-borne encephalitis virus
Uukuniemi virus

Documents & Links

10.1016/j.ttbdis.2019.05.005

Open Access VersionAccepted manuscript, 522 KBLicence: CC BY-NC-ND

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Cite this

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title = "Field samplings of Ixodes ricinus ticks from a tick-borne encephalitis virus micro-focus in Northern Zealand, Denmark",

abstract = "In 2008–2009 a tick-borne encephalitis virus (TBEV)micro-focus was detected in Northern Zealand, Denmark. No new cases of TBE with an epidemiological link to Northern Zealand has been reported since. Here we undertook to investigate Ixodes ricinus ticks from this endemic micro-focus in 2016 and 2017. In addition to TBEV, I. ricinus ticks may host other pathogens that include Borrelia spp., Babesia spp., Rickettsia spp. and Neoehrlichia mikurensis, together with various endosymbiont microorganisms. To detect multiple organisms we used a metagenomics PanVirus microarray and next-generation sequencing to examine the persistence and evolution of other emerging viruses, bacteria and parasites. Here we report the rise and fall of the Danish TBEV micro-focus in Northern Zealand. However, we identify for the first time in Danish I. ricinus ticks the presence of Uukuniemi virus in addition to a tick-borne phlebovirus and a range of bacteria.",

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author = "Andreas Petersen and Rosenstierne, \{Maiken Wors{\o}e\} and Morten Rasmussen and Kurt Fuursted and Nielsen, \{Henrik Vedel\} and \{O'Brien Andersen\}, Lee and Ren{\'e} B{\o}dker and Anders Fomsgaard",

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T1 - Field samplings of Ixodes ricinus ticks from a tick-borne encephalitis virus micro-focus in Northern Zealand, Denmark

AU - Petersen, Andreas

AU - Rosenstierne, Maiken Worsøe

AU - Rasmussen, Morten

AU - Fuursted, Kurt

AU - Nielsen, Henrik Vedel

AU - O'Brien Andersen, Lee

AU - Bødker, René

AU - Fomsgaard, Anders

PY - 2019/8/1

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N2 - In 2008–2009 a tick-borne encephalitis virus (TBEV)micro-focus was detected in Northern Zealand, Denmark. No new cases of TBE with an epidemiological link to Northern Zealand has been reported since. Here we undertook to investigate Ixodes ricinus ticks from this endemic micro-focus in 2016 and 2017. In addition to TBEV, I. ricinus ticks may host other pathogens that include Borrelia spp., Babesia spp., Rickettsia spp. and Neoehrlichia mikurensis, together with various endosymbiont microorganisms. To detect multiple organisms we used a metagenomics PanVirus microarray and next-generation sequencing to examine the persistence and evolution of other emerging viruses, bacteria and parasites. Here we report the rise and fall of the Danish TBEV micro-focus in Northern Zealand. However, we identify for the first time in Danish I. ricinus ticks the presence of Uukuniemi virus in addition to a tick-borne phlebovirus and a range of bacteria.

AB - In 2008–2009 a tick-borne encephalitis virus (TBEV)micro-focus was detected in Northern Zealand, Denmark. No new cases of TBE with an epidemiological link to Northern Zealand has been reported since. Here we undertook to investigate Ixodes ricinus ticks from this endemic micro-focus in 2016 and 2017. In addition to TBEV, I. ricinus ticks may host other pathogens that include Borrelia spp., Babesia spp., Rickettsia spp. and Neoehrlichia mikurensis, together with various endosymbiont microorganisms. To detect multiple organisms we used a metagenomics PanVirus microarray and next-generation sequencing to examine the persistence and evolution of other emerging viruses, bacteria and parasites. Here we report the rise and fall of the Danish TBEV micro-focus in Northern Zealand. However, we identify for the first time in Danish I. ricinus ticks the presence of Uukuniemi virus in addition to a tick-borne phlebovirus and a range of bacteria.

KW - Bacteria

KW - Ixodes ricinus

KW - Micro-focus

KW - Tick-borne encephalitis virus

KW - Uukuniemi virus

U2 - 10.1016/j.ttbdis.2019.05.005

DO - 10.1016/j.ttbdis.2019.05.005

M3 - Journal article

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SN - 1877-959X

VL - 10

SP - 1028

EP - 1032

JO - Ticks and Tick-borne Diseases

JF - Ticks and Tick-borne Diseases

IS - 5

ER -

Field samplings of Ixodes ricinus ticks from a tick-borne encephalitis virus micro-focus in Northern Zealand, Denmark

Abstract

Funding

Keywords

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