FGF2 and dual agonist of NCAM and FGF receptor 1, Enreptin, rescue neurite outgrowth loss in hippocampal neurons expressing mutated huntingtin proteins

Mirolyuba Ilieva; Troels T. Nielsen; Tanja Michel; Stanislava Pankratova

doi:10.1007/s00702-019-02073-1

FGF2 and dual agonist of NCAM and FGF receptor 1, Enreptin, rescue neurite outgrowth loss in hippocampal neurons expressing mutated huntingtin proteins

Mirolyuba Ilieva^*, Troels T. Nielsen, Tanja Michel, Stanislava Pankratova

^*Corresponding author for this work

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

In the present study, we developed an in vitro model of Huntington disease (HD) by transfecting primary rat hippocampal neurons with plasmids coding for m-htt exon 1 with different number of CAG repeats (18, 50 and 115) and demonstrated the influence of the length of polyQ sequence on neurite elongation. We found that exogenously applied FGF2 significantly rescued the m-htt-induced loss of neurite outgrowth. Moreover, the Enreptin peptide, an FGFR1 and NCAM dual agonist, had a similar neuritogenic effect to FGF2 in clinically relevant m-htt 50Q-expressing neurons. This study has developed an in vitro model of primary hippocampal neurons transfected with m-htt-coding vectors that is a powerful tool to study m-htt–related effects on neuronal placticity.

Original language	English
Journal	Journal of Neural Transmission
Volume	126
Issue number	11
Pages (from-to)	1493-1500
ISSN	0300-9564
DOIs	https://doi.org/10.1007/s00702-019-02073-1
Publication status	Published - Nov 2019

Keywords

Enreptin
FGF2
Hippocampal neurons
Huntington disease
Mutant huntingtin
Neurite outgrowth

Documents & Links

10.1007/s00702-019-02073-1

Cite this

@article{ca7d4d57984c45868590e1931d0091ec,

title = "FGF2 and dual agonist of NCAM and FGF receptor 1, Enreptin, rescue neurite outgrowth loss in hippocampal neurons expressing mutated huntingtin proteins",

abstract = "In the present study, we developed an in vitro model of Huntington disease (HD) by transfecting primary rat hippocampal neurons with plasmids coding for m-htt exon 1 with different number of CAG repeats (18, 50 and 115) and demonstrated the influence of the length of polyQ sequence on neurite elongation. We found that exogenously applied FGF2 significantly rescued the m-htt-induced loss of neurite outgrowth. Moreover, the Enreptin peptide, an FGFR1 and NCAM dual agonist, had a similar neuritogenic effect to FGF2 in clinically relevant m-htt 50Q-expressing neurons. This study has developed an in vitro model of primary hippocampal neurons transfected with m-htt-coding vectors that is a powerful tool to study m-htt–related effects on neuronal placticity.",

keywords = "Enreptin, FGF2, Hippocampal neurons, Huntington disease, Mutant huntingtin, Neurite outgrowth",

author = "Mirolyuba Ilieva and Nielsen, {Troels T.} and Tanja Michel and Stanislava Pankratova",

year = "2019",

month = nov,

doi = "10.1007/s00702-019-02073-1",

language = "English",

volume = "126",

pages = "1493--1500",

journal = "Journal of Neural Transmission",

issn = "0300-9564",

publisher = "Springer",

number = "11",

}

FGF2 and dual agonist of NCAM and FGF receptor 1, Enreptin, rescue neurite outgrowth loss in hippocampal neurons expressing mutated huntingtin proteins. / Ilieva, Mirolyuba; Nielsen, Troels T.; Michel, Tanja et al.
In: Journal of Neural Transmission, Vol. 126, No. 11, 11.2019, p. 1493-1500.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - FGF2 and dual agonist of NCAM and FGF receptor 1, Enreptin, rescue neurite outgrowth loss in hippocampal neurons expressing mutated huntingtin proteins

AU - Ilieva, Mirolyuba

AU - Nielsen, Troels T.

AU - Michel, Tanja

AU - Pankratova, Stanislava

PY - 2019/11

Y1 - 2019/11

N2 - In the present study, we developed an in vitro model of Huntington disease (HD) by transfecting primary rat hippocampal neurons with plasmids coding for m-htt exon 1 with different number of CAG repeats (18, 50 and 115) and demonstrated the influence of the length of polyQ sequence on neurite elongation. We found that exogenously applied FGF2 significantly rescued the m-htt-induced loss of neurite outgrowth. Moreover, the Enreptin peptide, an FGFR1 and NCAM dual agonist, had a similar neuritogenic effect to FGF2 in clinically relevant m-htt 50Q-expressing neurons. This study has developed an in vitro model of primary hippocampal neurons transfected with m-htt-coding vectors that is a powerful tool to study m-htt–related effects on neuronal placticity.

AB - In the present study, we developed an in vitro model of Huntington disease (HD) by transfecting primary rat hippocampal neurons with plasmids coding for m-htt exon 1 with different number of CAG repeats (18, 50 and 115) and demonstrated the influence of the length of polyQ sequence on neurite elongation. We found that exogenously applied FGF2 significantly rescued the m-htt-induced loss of neurite outgrowth. Moreover, the Enreptin peptide, an FGFR1 and NCAM dual agonist, had a similar neuritogenic effect to FGF2 in clinically relevant m-htt 50Q-expressing neurons. This study has developed an in vitro model of primary hippocampal neurons transfected with m-htt-coding vectors that is a powerful tool to study m-htt–related effects on neuronal placticity.

KW - Enreptin

KW - FGF2

KW - Hippocampal neurons

KW - Huntington disease

KW - Mutant huntingtin

KW - Neurite outgrowth

U2 - 10.1007/s00702-019-02073-1

DO - 10.1007/s00702-019-02073-1

M3 - Journal article

C2 - 31501979

AN - SCOPUS:85072037660

SN - 0300-9564

VL - 126

SP - 1493

EP - 1500

JO - Journal of Neural Transmission

JF - Journal of Neural Transmission

IS - 11

ER -

FGF2 and dual agonist of NCAM and FGF receptor 1, Enreptin, rescue neurite outgrowth loss in hippocampal neurons expressing mutated huntingtin proteins

Abstract

Keywords

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