Femtosecond laser micro-machined polyimide films for cell scaffold applications

Ieva Antanavičiute, Linas Šimatonis, Orestas Ulčinas, Aušra Gadeikyte, Brigita Abakevičiene, Sigitas Tamulevičius, Valeryia Mikalayeva, Vytenis Arvydas Skeberdis, Edgaras Stankevičius, Tomas Tamulevičius*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Engineering of sophisticated synthetic 3D scaffolds that allow controlling behaviour and location of the cells requires advanced micro/nano-fabrication techniques. Ultrafast laser micro-machining employing a 1030-nm wavelength Yb:KGW femtosecond laser and a micro-fabrication workstation for micro-machining of commercially available 12.7 and 25.4μm thickness polyimide (PI) film was applied. Mechanical properties of the fabricated scaffolds, i.e. arrays of differently spaced holes, were examined via custom-built uniaxial micro-tensile testing and finite element method simulations. We demonstrate that experimental micro-tensile testing results could be numerically simulated and explained by two-material model, assuming that 2-6μm width rings around the holes possessed up to five times higher Young's modulus and yield stress compared with the rest of the laser intacted PI film areas of 'dog-bone'-shaped specimens. That was attributed to material modification around the micro-machined holes in the vicinity of the position of the focused laser beam track during trepanning drilling. We demonstrate that virgin PI films provide a suitable environment for the mobility, proliferation and intercellular communication of human bone marrow mesenchymal stem cells, and discuss how cell behaviour varies on the micro-machined PI films with holes of different diameters (3.1, 8.4 and 16.7 μm) and hole spacing (30, 35, 40 and 45μm). We conclude that the holes of 3.1μm diameter were sufficient for metabolic and genetic communication through membranous tunneling tubes between cells residing on the opposite sides of PI film, but prevented the trans-migration of cells through the holes.

Original languageEnglish
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume12
Issue number2
Pages (from-to)e760-e773
ISSN1932-6254
DOIs
Publication statusPublished - Feb 2018

Fingerprint

Ultrashort pulses
Polyimides
Scaffolds
Tensile testing
Machining
Bone
Elastic Modulus
Ultrafast lasers
Mesenchymal Stromal Cells
Microfabrication
Cell Movement
Communication
Stem cells
Nanotechnology
Laser beams
Dogs
Yield stress
Drilling
Elastic moduli
Cells

Keywords

  • Femtosecond laser
  • Intercellular communication
  • Membranous tunneling tubes
  • Mesenchymal stem cells
  • Micro-machining
  • Polyimide film
  • Young's modulus
  • membranous tunneling tubes
  • polyimide film
  • femtosecond laser
  • micro-machining
  • mesenchymal stem cells
  • intercellular communication

Cite this

Antanavičiute, I., Šimatonis, L., Ulčinas, O., Gadeikyte, A., Abakevičiene, B., Tamulevičius, S., ... Tamulevičius, T. (2018). Femtosecond laser micro-machined polyimide films for cell scaffold applications. Journal of Tissue Engineering and Regenerative Medicine, 12(2), e760-e773. https://doi.org/10.1002/term.2376
Antanavičiute, Ieva ; Šimatonis, Linas ; Ulčinas, Orestas ; Gadeikyte, Aušra ; Abakevičiene, Brigita ; Tamulevičius, Sigitas ; Mikalayeva, Valeryia ; Skeberdis, Vytenis Arvydas ; Stankevičius, Edgaras ; Tamulevičius, Tomas. / Femtosecond laser micro-machined polyimide films for cell scaffold applications. In: Journal of Tissue Engineering and Regenerative Medicine. 2018 ; Vol. 12, No. 2. pp. e760-e773.
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abstract = "Engineering of sophisticated synthetic 3D scaffolds that allow controlling behaviour and location of the cells requires advanced micro/nano-fabrication techniques. Ultrafast laser micro-machining employing a 1030-nm wavelength Yb:KGW femtosecond laser and a micro-fabrication workstation for micro-machining of commercially available 12.7 and 25.4μm thickness polyimide (PI) film was applied. Mechanical properties of the fabricated scaffolds, i.e. arrays of differently spaced holes, were examined via custom-built uniaxial micro-tensile testing and finite element method simulations. We demonstrate that experimental micro-tensile testing results could be numerically simulated and explained by two-material model, assuming that 2-6μm width rings around the holes possessed up to five times higher Young's modulus and yield stress compared with the rest of the laser intacted PI film areas of 'dog-bone'-shaped specimens. That was attributed to material modification around the micro-machined holes in the vicinity of the position of the focused laser beam track during trepanning drilling. We demonstrate that virgin PI films provide a suitable environment for the mobility, proliferation and intercellular communication of human bone marrow mesenchymal stem cells, and discuss how cell behaviour varies on the micro-machined PI films with holes of different diameters (3.1, 8.4 and 16.7 μm) and hole spacing (30, 35, 40 and 45μm). We conclude that the holes of 3.1μm diameter were sufficient for metabolic and genetic communication through membranous tunneling tubes between cells residing on the opposite sides of PI film, but prevented the trans-migration of cells through the holes.",
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author = "Ieva Antanavičiute and Linas Šimatonis and Orestas Ulčinas and Aušra Gadeikyte and Brigita Abakevičiene and Sigitas Tamulevičius and Valeryia Mikalayeva and Skeberdis, {Vytenis Arvydas} and Edgaras Stankevičius and Tomas Tamulevičius",
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Antanavičiute, I, Šimatonis, L, Ulčinas, O, Gadeikyte, A, Abakevičiene, B, Tamulevičius, S, Mikalayeva, V, Skeberdis, VA, Stankevičius, E & Tamulevičius, T 2018, 'Femtosecond laser micro-machined polyimide films for cell scaffold applications', Journal of Tissue Engineering and Regenerative Medicine, vol. 12, no. 2, pp. e760-e773. https://doi.org/10.1002/term.2376

Femtosecond laser micro-machined polyimide films for cell scaffold applications. / Antanavičiute, Ieva; Šimatonis, Linas; Ulčinas, Orestas; Gadeikyte, Aušra; Abakevičiene, Brigita; Tamulevičius, Sigitas; Mikalayeva, Valeryia; Skeberdis, Vytenis Arvydas; Stankevičius, Edgaras; Tamulevičius, Tomas.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 2, 02.2018, p. e760-e773.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Femtosecond laser micro-machined polyimide films for cell scaffold applications

AU - Antanavičiute, Ieva

AU - Šimatonis, Linas

AU - Ulčinas, Orestas

AU - Gadeikyte, Aušra

AU - Abakevičiene, Brigita

AU - Tamulevičius, Sigitas

AU - Mikalayeva, Valeryia

AU - Skeberdis, Vytenis Arvydas

AU - Stankevičius, Edgaras

AU - Tamulevičius, Tomas

PY - 2018/2

Y1 - 2018/2

N2 - Engineering of sophisticated synthetic 3D scaffolds that allow controlling behaviour and location of the cells requires advanced micro/nano-fabrication techniques. Ultrafast laser micro-machining employing a 1030-nm wavelength Yb:KGW femtosecond laser and a micro-fabrication workstation for micro-machining of commercially available 12.7 and 25.4μm thickness polyimide (PI) film was applied. Mechanical properties of the fabricated scaffolds, i.e. arrays of differently spaced holes, were examined via custom-built uniaxial micro-tensile testing and finite element method simulations. We demonstrate that experimental micro-tensile testing results could be numerically simulated and explained by two-material model, assuming that 2-6μm width rings around the holes possessed up to five times higher Young's modulus and yield stress compared with the rest of the laser intacted PI film areas of 'dog-bone'-shaped specimens. That was attributed to material modification around the micro-machined holes in the vicinity of the position of the focused laser beam track during trepanning drilling. We demonstrate that virgin PI films provide a suitable environment for the mobility, proliferation and intercellular communication of human bone marrow mesenchymal stem cells, and discuss how cell behaviour varies on the micro-machined PI films with holes of different diameters (3.1, 8.4 and 16.7 μm) and hole spacing (30, 35, 40 and 45μm). We conclude that the holes of 3.1μm diameter were sufficient for metabolic and genetic communication through membranous tunneling tubes between cells residing on the opposite sides of PI film, but prevented the trans-migration of cells through the holes.

AB - Engineering of sophisticated synthetic 3D scaffolds that allow controlling behaviour and location of the cells requires advanced micro/nano-fabrication techniques. Ultrafast laser micro-machining employing a 1030-nm wavelength Yb:KGW femtosecond laser and a micro-fabrication workstation for micro-machining of commercially available 12.7 and 25.4μm thickness polyimide (PI) film was applied. Mechanical properties of the fabricated scaffolds, i.e. arrays of differently spaced holes, were examined via custom-built uniaxial micro-tensile testing and finite element method simulations. We demonstrate that experimental micro-tensile testing results could be numerically simulated and explained by two-material model, assuming that 2-6μm width rings around the holes possessed up to five times higher Young's modulus and yield stress compared with the rest of the laser intacted PI film areas of 'dog-bone'-shaped specimens. That was attributed to material modification around the micro-machined holes in the vicinity of the position of the focused laser beam track during trepanning drilling. We demonstrate that virgin PI films provide a suitable environment for the mobility, proliferation and intercellular communication of human bone marrow mesenchymal stem cells, and discuss how cell behaviour varies on the micro-machined PI films with holes of different diameters (3.1, 8.4 and 16.7 μm) and hole spacing (30, 35, 40 and 45μm). We conclude that the holes of 3.1μm diameter were sufficient for metabolic and genetic communication through membranous tunneling tubes between cells residing on the opposite sides of PI film, but prevented the trans-migration of cells through the holes.

KW - Femtosecond laser

KW - Intercellular communication

KW - Membranous tunneling tubes

KW - Mesenchymal stem cells

KW - Micro-machining

KW - Polyimide film

KW - Young's modulus

KW - membranous tunneling tubes

KW - polyimide film

KW - femtosecond laser

KW - micro-machining

KW - mesenchymal stem cells

KW - intercellular communication

U2 - 10.1002/term.2376

DO - 10.1002/term.2376

M3 - Journal article

VL - 12

SP - e760-e773

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

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