Favorable prognostic impact of Natural Killer cells and T cells in high-grade serous ovarian carcinoma

Jon Røikjaer Henriksen*, Frede Donskov, Marianne Waldstrøm, Anders Jakobsen, Mette Hjortkjaer, Christina Braad Petersen, Karina Dahl Steffensen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Introduction: The aim of the present study was to investigate the prognostic impact of intratumoral cytotoxic T cells, Natural Killer (NK) cells, neutrophils and PD-L1 expression in patients with epithelial ovarian cancer. Methods: All patients diagnosed with high-grade serous carcinoma (HGSC) in Denmark in 2005 were included in the study. Immunohistochemical staining for PD-L1, CD8, CD66b and CD57 was performed on tumor tissue from 283 patients. Cell densities were analyzed using a digital image analysis method. The primary endpoint was overall survival (OS). Results: The median OS for HGSC patients was 30 months. It was 45 months in patients with high level of CD57+ NK cells (≥10 cells/mm 2) compared with 29 month in patients with low level (<10 cells/mm 2) (p =.0310). The median OS was 37 and 25 months in patients with high vs. low level of CD8+ T cells (cutoff 80 cells/mm 2) (p =.0008). In multivariate analysis, high numbers of CD57+ NK cells and CD8+ T cells remained independent markers of favorable OS, adjusted hazard ratio (HR) 0.67; p =.041, and HR 0.72; p =.020, respectively. PD-L1 expression was associated with improved OS (37 months vs. 22 months, p =.0006), but was only borderline significant in the multivariate analysis (HR 0.77, p =.061). CD66b + neutrophils had no association with OS. Conclusions: In patients with HGSC tumor-infiltrating CD57+ NK cells and CD8+ T cells had favorable prognostic impact, while PD-L1 expression had borderline favorable prognostic significance. CD66b + neutrophils had no prognostic association. These findings may influence future immunotherapy development.

Original languageEnglish
JournalActa oncologica (Stockholm, Sweden)
Pages (from-to)1-8
Number of pages8
ISSN0284-186X
DOIs
Publication statusE-pub ahead of print - 14. Jan 2020

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