False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients

Silje Vermedal Høgh; Charlotte Nielsen Agergaard; Marianne Nielsine Skov; Michael Kemp

doi:10.2174/1874285801913010051

False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients

Silje Vermedal Høgh, Charlotte Nielsen Agergaard, Marianne Nielsine Skov, Michael Kemp

KI, OUH, Research unit of Clinical Microbiology (Odense)

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001. Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis. Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.

Original language	English
Journal	The Open Microbiology Journal
Volume	13
Pages (from-to)	51-54
ISSN	1874-2858
DOIs	https://doi.org/10.2174/1874285801913010051
Publication status	Published - Apr 2019

Keywords

Bordetella pertussis
False-positive
IS1001
IS1002
IS481
Real-time PCR

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10.2174/1874285801913010051Licence: CC BY

False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish PatientsFinal published version, 226 KBLicence: CC BY

Cite this

@article{38b60046bbaf4a6b8f4f80391cbd95e3,

title = "False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients",

abstract = "Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001. Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis. Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.",

keywords = "Bordetella pertussis, False-positive, IS1001, IS1002, IS481, Real-time PCR",

author = "H{\o}gh, {Silje Vermedal} and Agergaard, {Charlotte Nielsen} and Skov, {Marianne Nielsine} and Michael Kemp",

year = "2019",

month = apr,

doi = "10.2174/1874285801913010051",

language = "English",

volume = "13",

pages = "51--54",

journal = "The Open Microbiology Journal",

issn = "1874-2858",

publisher = "Bentham Open",

}

TY - JOUR

T1 - False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients

AU - Høgh, Silje Vermedal

AU - Agergaard, Charlotte Nielsen

AU - Skov, Marianne Nielsine

AU - Kemp, Michael

PY - 2019/4

Y1 - 2019/4

N2 - Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001. Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis. Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.

AB - Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001. Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis. Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.

KW - Bordetella pertussis

KW - False-positive

KW - IS1001

KW - IS1002

KW - IS481

KW - Real-time PCR

U2 - 10.2174/1874285801913010051

DO - 10.2174/1874285801913010051

M3 - Journal article

SN - 1874-2858

VL - 13

SP - 51

EP - 54

JO - The Open Microbiology Journal

JF - The Open Microbiology Journal

ER -

False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients

Abstract

Keywords

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