Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001. Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis. Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.
- Bordetella pertussis
- Real-time PCR