Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

Yangxin Wang, Ningning Zhang, Deming Tan, Zhenhui Qi, Changzhu Wu*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

Original languageEnglish
Article number714
JournalFrontiers in Bioengineering and Biotechnology
Volume8
ISSN2296-4185
DOIs
Publication statusPublished - 7. Jul 2020

Keywords

  • biocatalysis
  • enzymatic reactions
  • heterogeneous catalysis
  • metal–organic frameworks
  • size-selective catalysis

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