Extra-cellular vesicles carry proteome of cancer hallmarks

Ana Sofia Carvalho, Henrique Baeta, Bruno Costa Silva, Maria Carolina Strano Moraes, Cristian Bodo, Hans Christian Beck, Manuel S Rodriguez, Mayank Saraswat, Akhilesh Pandey, Rune Matthiesen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Through lateral transfer, extra-cellular vesicles (EVs) transport their DNA, miRNA, mRNA and proteins such as enzymes mediating drug resistance, transporters as well as growth factors to neighboring cells. By virtue of this horizontal transfer, EVs potentially regulate cell growth, migration, angiogenesis and metastasis and increase tissue permeability in cancer. Furthermore, EVs regulate immune factors and allow the tumor cells to evade immune recognition and cell death. To explore if the proteomes of exosomes support functional transfer of cancer hallmarks, in this meta-analysis, we compared EVs and whole cell proteomes from the NCI-60 human tumor cell line panel. We observed a subgroup of proteins in each cancer hallmark signature as highly abundant and consistently expressed in EVs from all cell lines. Among these were oncoproteins frequently targeted in cancer therapies whose presence on EVs could potentially render therapies less effective by serving as decoys.

Original languageEnglish
JournalFrontiers in Bioscience
Volume25
Pages (from-to)398-436
Number of pages39
ISSN1093-9946
DOIs
Publication statusPublished - 1. Jan 2020

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Proteome
Cells
Tumors
Neoplasms
Oncogene Proteins
Immunologic Factors
Cell growth
Cell death
MicroRNAs
Intercellular Signaling Peptides and Proteins
Exosomes
Proteins
Tissue
R Factors
Messenger RNA
Tumor Cell Line
Drug Resistance
DNA
Cell Movement
Enzymes

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Carvalho, A. S., Baeta, H., Silva, B. C., Moraes, M. C. S., Bodo, C., Beck, H. C., ... Matthiesen, R. (2020). Extra-cellular vesicles carry proteome of cancer hallmarks. Frontiers in Bioscience, 25, 398-436. https://doi.org/10.2741/4811
Carvalho, Ana Sofia ; Baeta, Henrique ; Silva, Bruno Costa ; Moraes, Maria Carolina Strano ; Bodo, Cristian ; Beck, Hans Christian ; Rodriguez, Manuel S ; Saraswat, Mayank ; Pandey, Akhilesh ; Matthiesen, Rune. / Extra-cellular vesicles carry proteome of cancer hallmarks. In: Frontiers in Bioscience. 2020 ; Vol. 25. pp. 398-436.
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Carvalho, AS, Baeta, H, Silva, BC, Moraes, MCS, Bodo, C, Beck, HC, Rodriguez, MS, Saraswat, M, Pandey, A & Matthiesen, R 2020, 'Extra-cellular vesicles carry proteome of cancer hallmarks', Frontiers in Bioscience, vol. 25, pp. 398-436. https://doi.org/10.2741/4811

Extra-cellular vesicles carry proteome of cancer hallmarks. / Carvalho, Ana Sofia; Baeta, Henrique; Silva, Bruno Costa; Moraes, Maria Carolina Strano; Bodo, Cristian; Beck, Hans Christian; Rodriguez, Manuel S; Saraswat, Mayank; Pandey, Akhilesh; Matthiesen, Rune.

In: Frontiers in Bioscience, Vol. 25, 01.01.2020, p. 398-436.

Research output: Contribution to journalJournal articleResearchpeer-review

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AU - Carvalho, Ana Sofia

AU - Baeta, Henrique

AU - Silva, Bruno Costa

AU - Moraes, Maria Carolina Strano

AU - Bodo, Cristian

AU - Beck, Hans Christian

AU - Rodriguez, Manuel S

AU - Saraswat, Mayank

AU - Pandey, Akhilesh

AU - Matthiesen, Rune

PY - 2020/1/1

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N2 - Through lateral transfer, extra-cellular vesicles (EVs) transport their DNA, miRNA, mRNA and proteins such as enzymes mediating drug resistance, transporters as well as growth factors to neighboring cells. By virtue of this horizontal transfer, EVs potentially regulate cell growth, migration, angiogenesis and metastasis and increase tissue permeability in cancer. Furthermore, EVs regulate immune factors and allow the tumor cells to evade immune recognition and cell death. To explore if the proteomes of exosomes support functional transfer of cancer hallmarks, in this meta-analysis, we compared EVs and whole cell proteomes from the NCI-60 human tumor cell line panel. We observed a subgroup of proteins in each cancer hallmark signature as highly abundant and consistently expressed in EVs from all cell lines. Among these were oncoproteins frequently targeted in cancer therapies whose presence on EVs could potentially render therapies less effective by serving as decoys.

AB - Through lateral transfer, extra-cellular vesicles (EVs) transport their DNA, miRNA, mRNA and proteins such as enzymes mediating drug resistance, transporters as well as growth factors to neighboring cells. By virtue of this horizontal transfer, EVs potentially regulate cell growth, migration, angiogenesis and metastasis and increase tissue permeability in cancer. Furthermore, EVs regulate immune factors and allow the tumor cells to evade immune recognition and cell death. To explore if the proteomes of exosomes support functional transfer of cancer hallmarks, in this meta-analysis, we compared EVs and whole cell proteomes from the NCI-60 human tumor cell line panel. We observed a subgroup of proteins in each cancer hallmark signature as highly abundant and consistently expressed in EVs from all cell lines. Among these were oncoproteins frequently targeted in cancer therapies whose presence on EVs could potentially render therapies less effective by serving as decoys.

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Carvalho AS, Baeta H, Silva BC, Moraes MCS, Bodo C, Beck HC et al. Extra-cellular vesicles carry proteome of cancer hallmarks. Frontiers in Bioscience. 2020 Jan 1;25:398-436. https://doi.org/10.2741/4811