Expression of a starch-binding factor (CBM20) in plastids control the number of starch granules and the level of CO2 fixation

Yingxin Zhong, Domenico Sagnelli, Henrik Bak Topbjerg, Harald Hasler-Sheetal, Olga Andrzejzcak, Kourosh Hooshmand, René Gislum, Dong Jiang, Ian Max Møller, Andreas Blennow, Kim Henrik Hebelstrup

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Abstract

Starch granule biosynthesis in plant plastids is coordinated by an orchestrated action of transferases, hydrolases and dikinases. These enzymes either contain starch-binding domain(s) themselves, or are dependent on direct interaction with co-factors containing starch-binding domains. As a means to competitively interfere with existing starch-protein interactions in plastids we expressed the protein module CBM20, which has a very high affinity for starch, ectopically in barley plastids. This interference resulted in an increase in the number of starch granules in chloroplasts and formation of compound starch granules in barley grain amyloplasts, which is unusual for barley. More importantly, we observed a photosystem-independent inhibition of CO2 fixation, with a subsequent reduced growth rate and lower accumulation of carbohydrates with effects throughout the metabolome, including lower accumulation of transient leaf starch. Our results demonstrate the importance of endogenous starch-protein interactions for controlling starch granule morphology, starch granule number and plant growth as substantiated by a metabolic link between starch-protein interaction and control of CO2 fixation in chloroplasts.

Original languageEnglish
JournalJournal of Experimental Botany
ISSN0022-0957
DOIs
Publication statusE-pub ahead of print - 8. Sep 2019

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Chloroplast Proteins
Proteins
Hydrolases
Growth
Enzymes

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Zhong, Yingxin ; Sagnelli, Domenico ; Topbjerg, Henrik Bak ; Hasler-Sheetal, Harald ; Andrzejzcak, Olga ; Hooshmand, Kourosh ; Gislum, René ; Jiang, Dong ; Møller, Ian Max ; Blennow, Andreas ; Hebelstrup, Kim Henrik. / Expression of a starch-binding factor (CBM20) in plastids control the number of starch granules and the level of CO2 fixation. In: Journal of Experimental Botany. 2019.
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title = "Expression of a starch-binding factor (CBM20) in plastids control the number of starch granules and the level of CO2 fixation",
abstract = "Starch granule biosynthesis in plant plastids is coordinated by an orchestrated action of transferases, hydrolases and dikinases. These enzymes either contain starch-binding domain(s) themselves, or are dependent on direct interaction with co-factors containing starch-binding domains. As a means to competitively interfere with existing starch-protein interactions in plastids we expressed the protein module CBM20, which has a very high affinity for starch, ectopically in barley plastids. This interference resulted in an increase in the number of starch granules in chloroplasts and formation of compound starch granules in barley grain amyloplasts, which is unusual for barley. More importantly, we observed a photosystem-independent inhibition of CO2 fixation, with a subsequent reduced growth rate and lower accumulation of carbohydrates with effects throughout the metabolome, including lower accumulation of transient leaf starch. Our results demonstrate the importance of endogenous starch-protein interactions for controlling starch granule morphology, starch granule number and plant growth as substantiated by a metabolic link between starch-protein interaction and control of CO2 fixation in chloroplasts.",
author = "Yingxin Zhong and Domenico Sagnelli and Topbjerg, {Henrik Bak} and Harald Hasler-Sheetal and Olga Andrzejzcak and Kourosh Hooshmand and Ren{\'e} Gislum and Dong Jiang and M{\o}ller, {Ian Max} and Andreas Blennow and Hebelstrup, {Kim Henrik}",
note = "{\circledC} The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.",
year = "2019",
month = "9",
day = "8",
doi = "10.1093/jxb/erz401",
language = "English",
journal = "Journal of Experimental Botany",
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Zhong, Y, Sagnelli, D, Topbjerg, HB, Hasler-Sheetal, H, Andrzejzcak, O, Hooshmand, K, Gislum, R, Jiang, D, Møller, IM, Blennow, A & Hebelstrup, KH 2019, 'Expression of a starch-binding factor (CBM20) in plastids control the number of starch granules and the level of CO2 fixation', Journal of Experimental Botany. https://doi.org/10.1093/jxb/erz401

Expression of a starch-binding factor (CBM20) in plastids control the number of starch granules and the level of CO2 fixation. / Zhong, Yingxin; Sagnelli, Domenico; Topbjerg, Henrik Bak; Hasler-Sheetal, Harald; Andrzejzcak, Olga; Hooshmand, Kourosh; Gislum, René; Jiang, Dong; Møller, Ian Max; Blennow, Andreas; Hebelstrup, Kim Henrik.

In: Journal of Experimental Botany, 08.09.2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Expression of a starch-binding factor (CBM20) in plastids control the number of starch granules and the level of CO2 fixation

AU - Zhong, Yingxin

AU - Sagnelli, Domenico

AU - Topbjerg, Henrik Bak

AU - Hasler-Sheetal, Harald

AU - Andrzejzcak, Olga

AU - Hooshmand, Kourosh

AU - Gislum, René

AU - Jiang, Dong

AU - Møller, Ian Max

AU - Blennow, Andreas

AU - Hebelstrup, Kim Henrik

N1 - © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.

PY - 2019/9/8

Y1 - 2019/9/8

N2 - Starch granule biosynthesis in plant plastids is coordinated by an orchestrated action of transferases, hydrolases and dikinases. These enzymes either contain starch-binding domain(s) themselves, or are dependent on direct interaction with co-factors containing starch-binding domains. As a means to competitively interfere with existing starch-protein interactions in plastids we expressed the protein module CBM20, which has a very high affinity for starch, ectopically in barley plastids. This interference resulted in an increase in the number of starch granules in chloroplasts and formation of compound starch granules in barley grain amyloplasts, which is unusual for barley. More importantly, we observed a photosystem-independent inhibition of CO2 fixation, with a subsequent reduced growth rate and lower accumulation of carbohydrates with effects throughout the metabolome, including lower accumulation of transient leaf starch. Our results demonstrate the importance of endogenous starch-protein interactions for controlling starch granule morphology, starch granule number and plant growth as substantiated by a metabolic link between starch-protein interaction and control of CO2 fixation in chloroplasts.

AB - Starch granule biosynthesis in plant plastids is coordinated by an orchestrated action of transferases, hydrolases and dikinases. These enzymes either contain starch-binding domain(s) themselves, or are dependent on direct interaction with co-factors containing starch-binding domains. As a means to competitively interfere with existing starch-protein interactions in plastids we expressed the protein module CBM20, which has a very high affinity for starch, ectopically in barley plastids. This interference resulted in an increase in the number of starch granules in chloroplasts and formation of compound starch granules in barley grain amyloplasts, which is unusual for barley. More importantly, we observed a photosystem-independent inhibition of CO2 fixation, with a subsequent reduced growth rate and lower accumulation of carbohydrates with effects throughout the metabolome, including lower accumulation of transient leaf starch. Our results demonstrate the importance of endogenous starch-protein interactions for controlling starch granule morphology, starch granule number and plant growth as substantiated by a metabolic link between starch-protein interaction and control of CO2 fixation in chloroplasts.

U2 - 10.1093/jxb/erz401

DO - 10.1093/jxb/erz401

M3 - Journal article

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

ER -