Exploring environmental sources of archaeal lipids for improving liposomes for oral drug delivery

Mette Sloth Bohsen, Kenneth Hesseldahl Zipfel, Nerea Uri, Per Henrik Nielsen, Martin Brandl, Alexander H. Treusch

Research output: Contribution to conference without publisher/journalPosterResearch

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Abstract

While the oral route of drug administration is very cost efficient and has high patient compliance, challenges that especially modern therapeutic protein and peptide drugs face are, among others, premature degradation and poor permeability over the intestinal barrier. The use of nanoparticulate drug carriers like liposomes (spherical vesicles surrounded by a phospholipid bilayer) is a promising way of overcoming these challenges. Advanced liposome formulations often contain enhancers to further stabilize the lipid bilayer, with archaeal tetraether lipids (TELs) being a fairly recently established enhancer. TELs can be found in hyperthermophilic Crenarchaeota, however their demanding growth requirements make the biotechnological production of TELs so far uneconomical. Yet unexplored sources of TELs are environmental Thaumarchaeota, which can be found in many environments including the ocean, soil, freshwater and also wastewater treatment plants (WWTP). To explore if indeed TELs from Thaumarchaeota can be used as enhancers of liposome stability, we collected biomass from different biological treatments at the Ejby Mølle WWTP (Odense) and subsequently optimized protocols for extracting lipids, settling on a modified Blight & Dyer protocol. These efforts were guided by a screening of the biological treatments of the WWTP for Archaea, indicating the presence of Thaumarchaeota, although often at low abundances. The initial results of these studies will be presented and discussed.
Original languageEnglish
Publication date11. Nov 2019
Publication statusPublished - 11. Nov 2019
EventDanish Microbiological Society - DMS Annual Congress 2019: DMS Congress 2019 - Eigtveds Pakhus, København, Denmark
Duration: 11. Nov 201911. Nov 2019
https://dmselskab.dk/congress/

Conference

ConferenceDanish Microbiological Society - DMS Annual Congress 2019
LocationEigtveds Pakhus
Country/TerritoryDenmark
CityKøbenhavn
Period11/11/201911/11/2019
Internet address

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