Evaluation of siRNA stability and interaction with serum components using an agarose gel-based single-molecule FRET labeling method

Martina Tuttolomondo; Henrik J. Ditzel

doi:10.1007/978-1-0716-1298-9_4

Evaluation of siRNA stability and interaction with serum components using an agarose gel-based single-molecule FRET labeling method

Martina Tuttolomondo^*, Henrik J. Ditzel

^*Corresponding author for this work

KI, OUH, Research unit of Oncology (Odense)

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

Small interfering RNAs (siRNAs) are RNA molecules with promising therapeutic potential as a result of their selective mRNA cleavage. However, despite recent progress, low stability in the bloodstream is an impediment to successful administration in vivo. Thus, the availability of flexible and rapid methods for studying siRNA stability and vehicles is crucial for future novel siRNA-based therapeutics. Herein, we report a fast Förster resonance energy transfer (FRET) method based on agarose gel electrophoresis to evaluate the stability of siRNA in serum as well as siRNA interaction with serum proteins and enzymes.

Original language	English
Title of host publication	Design and Delivery of SiRNA Therapeutics
Editors	Henrik J. Ditzel, Martina Tuttolomondo, Sakari Kauppinen
Volume	2282
Publisher	Humana Press
Publication date	2021
Pages	43-56
ISBN (Print)	978-1-0716-1297-2
ISBN (Electronic)	978-1-0716-1298-9
DOIs	https://doi.org/10.1007/978-1-0716-1298-9_4
Publication status	Published - 2021

Series	Methods in Molecular Biology
Volume	2282
ISSN	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2021.

Keywords

Agarose gel electrophoresis
Förster resonance energy transfer
RNA interference
Serum protein corona
siRNA stability
Small interfering RNA

Documents & Links

10.1007/978-1-0716-1298-9_4

Cite this

Tuttolomondo, Martina ; Ditzel, Henrik J. / Evaluation of siRNA stability and interaction with serum components using an agarose gel-based single-molecule FRET labeling method. Design and Delivery of SiRNA Therapeutics. editor / Henrik J. Ditzel ; Martina Tuttolomondo ; Sakari Kauppinen. Vol. 2282 Humana Press, 2021. pp. 43-56 (Methods in Molecular Biology, Vol. 2282).

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abstract = "Small interfering RNAs (siRNAs) are RNA molecules with promising therapeutic potential as a result of their selective mRNA cleavage. However, despite recent progress, low stability in the bloodstream is an impediment to successful administration in vivo. Thus, the availability of flexible and rapid methods for studying siRNA stability and vehicles is crucial for future novel siRNA-based therapeutics. Herein, we report a fast F{\"o}rster resonance energy transfer (FRET) method based on agarose gel electrophoresis to evaluate the stability of siRNA in serum as well as siRNA interaction with serum proteins and enzymes.",

keywords = "Agarose gel electrophoresis, F{\"o}rster resonance energy transfer, RNA interference, Serum protein corona, siRNA stability, Small interfering RNA",

author = "Martina Tuttolomondo and Ditzel, {Henrik J.}",

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year = "2021",

doi = "10.1007/978-1-0716-1298-9_4",

language = "English",

isbn = " 978-1-0716-1297-2",

volume = "2282",

series = "Methods in Molecular Biology",

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Tuttolomondo, M & Ditzel, HJ 2021, Evaluation of siRNA stability and interaction with serum components using an agarose gel-based single-molecule FRET labeling method. in HJ Ditzel, M Tuttolomondo & S Kauppinen (eds), Design and Delivery of SiRNA Therapeutics. vol. 2282, Humana Press, Methods in Molecular Biology, vol. 2282, pp. 43-56. https://doi.org/10.1007/978-1-0716-1298-9_4

Evaluation of siRNA stability and interaction with serum components using an agarose gel-based single-molecule FRET labeling method. / Tuttolomondo, Martina ; Ditzel, Henrik J.
Design and Delivery of SiRNA Therapeutics. ed. / Henrik J. Ditzel; Martina Tuttolomondo; Sakari Kauppinen. Vol. 2282 Humana Press, 2021. p. 43-56 (Methods in Molecular Biology, Vol. 2282).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

TY - CHAP

T1 - Evaluation of siRNA stability and interaction with serum components using an agarose gel-based single-molecule FRET labeling method

AU - Tuttolomondo, Martina

AU - Ditzel, Henrik J.

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2021.

PY - 2021

Y1 - 2021

N2 - Small interfering RNAs (siRNAs) are RNA molecules with promising therapeutic potential as a result of their selective mRNA cleavage. However, despite recent progress, low stability in the bloodstream is an impediment to successful administration in vivo. Thus, the availability of flexible and rapid methods for studying siRNA stability and vehicles is crucial for future novel siRNA-based therapeutics. Herein, we report a fast Förster resonance energy transfer (FRET) method based on agarose gel electrophoresis to evaluate the stability of siRNA in serum as well as siRNA interaction with serum proteins and enzymes.

AB - Small interfering RNAs (siRNAs) are RNA molecules with promising therapeutic potential as a result of their selective mRNA cleavage. However, despite recent progress, low stability in the bloodstream is an impediment to successful administration in vivo. Thus, the availability of flexible and rapid methods for studying siRNA stability and vehicles is crucial for future novel siRNA-based therapeutics. Herein, we report a fast Förster resonance energy transfer (FRET) method based on agarose gel electrophoresis to evaluate the stability of siRNA in serum as well as siRNA interaction with serum proteins and enzymes.

KW - Agarose gel electrophoresis

KW - Förster resonance energy transfer

KW - RNA interference

KW - Serum protein corona

KW - siRNA stability

KW - Small interfering RNA

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DO - 10.1007/978-1-0716-1298-9_4

M3 - Book chapter

C2 - 33928569

AN - SCOPUS:85105159646

SN - 978-1-0716-1297-2

VL - 2282

T3 - Methods in Molecular Biology

SP - 43

EP - 56

BT - Design and Delivery of SiRNA Therapeutics

A2 - Ditzel, Henrik J.

A2 - Tuttolomondo, Martina

A2 - Kauppinen, Sakari

PB - Humana Press

ER -

Tuttolomondo M , Ditzel HJ. Evaluation of siRNA stability and interaction with serum components using an agarose gel-based single-molecule FRET labeling method. In Ditzel HJ, Tuttolomondo M, Kauppinen S, editors, Design and Delivery of SiRNA Therapeutics. Vol. 2282. Humana Press. 2021. p. 43-56. (Methods in Molecular Biology, Vol. 2282). doi: 10.1007/978-1-0716-1298-9_4

Evaluation of siRNA stability and interaction with serum components using an agarose gel-based single-molecule FRET labeling method

Abstract

Bibliographical note

Keywords

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