ESwab challenges influenza virus propagation in cell cultures

Ramona Trebbien, B Andersen, Jesper Rønn, Jacob L McCauley, T Kølsen Fischer

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Although the ESwab kit (Copan, Brescia, Italy) is intended for sampling bacteria for culture, this kit is increasingly also used for virus sampling. The effect of ESwab medium on influenza virus detection by real-time reverse transcription-polymerase chain reaction (RT-PCR) or virus propagation in Madin-Darby canine kidney (MDCK) cell culture was investigated. The ESwab medium was suitable for viral RNA detection but not for viral propagation due to cytotoxicity. Sampling influenza viruses with ESwab challenges influenza surveillance by strongly limiting the possibility of antigenic characterisation.

Original languageEnglish
JournalEurosurveillance (Online Edition)
Volume19
Issue number50
Pages (from-to)20995
ISSN1025-496X
DOIs
Publication statusPublished - 18. Dec 2014
Externally publishedYes

Keywords

  • Animals
  • Cell Culture Techniques
  • DNA, Viral
  • Dogs
  • Madin Darby Canine Kidney Cells
  • Orthomyxoviridae
  • Orthomyxoviridae Infections
  • RNA, Viral
  • Reverse Transcriptase Polymerase Chain Reaction
  • Journal Article
  • Research Support, Non-U.S. Gov't

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