Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells

Changzhu Wu; Christine  Strehmel; Katharina Achazi; Leonardo  Chiappisi; Jens  Dernedde; Marga  C. Lensen; Michael  Gradzielski; Marion B. Ansorge-Schumacher; Rainer Haag

doi:10.1021/bm500705x

Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells

Changzhu Wu, Christine Strehmel, Katharina Achazi, Leonardo Chiappisi, Jens Dernedde, Marga C. Lensen, Michael Gradzielski, Marion B. Ansorge-Schumacher, Rainer Haag

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Although several strategies are now available to enzymatically cross-link linear polymers to hydrogels for biomedical use, little progress has been reported on the use of dendritic polymers for the same purpose. Herein, we demonstrate that horseradish peroxidase (HRP) successfully catalyzes the oxidative cross-linking of a hyperbranched polyglycerol (hPG) functionalized with phenol groups to hydrogels. The tunable cross-linking results in adjustable hydrogel properties. Because the obtained materials are cytocompatible, they have great potential for encapsulating living cells for regenerative therapy. The gel formation can be triggered by glucose and controlled well under various environmental conditions.

Original language	English
Journal	Biomacromolecules
Volume	15
Issue number	11
Pages (from-to)	3881-3890
ISSN	1525-7797
DOIs	https://doi.org/10.1021/bm500705x
Publication status	Published - 21. Aug 2014
Externally published	Yes

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10.1021/bm500705x

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title = "Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells",

abstract = "Although several strategies are now available to enzymatically cross-link linear polymers to hydrogels for biomedical use, little progress has been reported on the use of dendritic polymers for the same purpose. Herein, we demonstrate that horseradish peroxidase (HRP) successfully catalyzes the oxidative cross-linking of a hyperbranched polyglycerol (hPG) functionalized with phenol groups to hydrogels. The tunable cross-linking results in adjustable hydrogel properties. Because the obtained materials are cytocompatible, they have great potential for encapsulating living cells for regenerative therapy. The gel formation can be triggered by glucose and controlled well under various environmental conditions.",

author = "Changzhu Wu and Christine Strehmel and Katharina Achazi and Leonardo Chiappisi and Jens Dernedde and {C. Lensen}, Marga and Michael Gradzielski and Ansorge-Schumacher, {Marion B.} and Rainer Haag",

year = "2014",

month = aug,

day = "21",

doi = "10.1021/bm500705x",

language = "English",

volume = "15",

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journal = "Biomacromolecules",

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TY - JOUR

T1 - Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells

AU - Wu, Changzhu

AU - Strehmel, Christine

AU - Achazi, Katharina

AU - Chiappisi, Leonardo

AU - Dernedde, Jens

AU - C. Lensen, Marga

AU - Gradzielski, Michael

AU - Ansorge-Schumacher, Marion B.

AU - Haag, Rainer

PY - 2014/8/21

Y1 - 2014/8/21

N2 - Although several strategies are now available to enzymatically cross-link linear polymers to hydrogels for biomedical use, little progress has been reported on the use of dendritic polymers for the same purpose. Herein, we demonstrate that horseradish peroxidase (HRP) successfully catalyzes the oxidative cross-linking of a hyperbranched polyglycerol (hPG) functionalized with phenol groups to hydrogels. The tunable cross-linking results in adjustable hydrogel properties. Because the obtained materials are cytocompatible, they have great potential for encapsulating living cells for regenerative therapy. The gel formation can be triggered by glucose and controlled well under various environmental conditions.

AB - Although several strategies are now available to enzymatically cross-link linear polymers to hydrogels for biomedical use, little progress has been reported on the use of dendritic polymers for the same purpose. Herein, we demonstrate that horseradish peroxidase (HRP) successfully catalyzes the oxidative cross-linking of a hyperbranched polyglycerol (hPG) functionalized with phenol groups to hydrogels. The tunable cross-linking results in adjustable hydrogel properties. Because the obtained materials are cytocompatible, they have great potential for encapsulating living cells for regenerative therapy. The gel formation can be triggered by glucose and controlled well under various environmental conditions.

U2 - 10.1021/bm500705x

DO - 10.1021/bm500705x

M3 - Journal article

SN - 1525-7797

VL - 15

SP - 3881

EP - 3890

JO - Biomacromolecules

JF - Biomacromolecules

IS - 11

ER -

Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells

Abstract

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