Enrichment of a solventogenic anaerobic sludge converting carbon monoxide and syngas into acids and alcohols

Samayita Chakraborty; Eldon R. Rene; Piet N.L. Lens; María C. Veiga; Christian Kennes

doi:10.1016/j.biortech.2018.10.002

Enrichment of a solventogenic anaerobic sludge converting carbon monoxide and syngas into acids and alcohols

Samayita Chakraborty
, Eldon R. Rene
, Piet N.L. Lens
, María C. Veiga
, Christian Kennes^*

^*Corresponding author for this work

University of A Coruña
UNESCO-IHE Institute for Water Education
University of A Coruña

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

An anaerobic granular sludge was acclimatized to utilise CO in a continuously gas-fed stirred tank bioreactor by applying operating conditions expected to stimulate solventogenesis, i.e. the production of alcohols, and allowing to enrich for solventogenic populations. A cycle of high (6.2) and low (4.9) pH was applied in order to produce volatile fatty acids first at high pH, followed by their bioconversion into alcohols at low pH. The addition of yeast extract stimulated biomass growth, but not necessarily solventogenesis. The highest concentrations of metabolites achieved were 6.18 g/L acetic acid (30th day), 1.18 g/L butyric acid (28th day), and 0.423 g/L hexanoic acid (32nd day). Subsequently, acids were metabolized at lower pH, producing alcohols at concentrations of 11.1 g/L ethanol (43rd day), 1.8 g/L butanol (41st day) and 1.46 g/L hexanol (42nd day), confirming the successful enrichment strategy. Similarly, the enriched sludge could also convert syngas into acids and alcohols.

Original language	English
Journal	Bioresource Technology
Volume	272
Pages (from-to)	130-136
Number of pages	7
ISSN	0960-8524
DOIs	https://doi.org/10.1016/j.biortech.2018.10.002
Publication status	Published - Jan 2019
Externally published	Yes

Bibliographical note

Funding Information:
This experimental work, undertaken at UDC (Spain), was financially supported by the Spanish Ministry of Economy, Industry and Competitiveness [project CTQ2017-88292-R ] and the Marie Skłodowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET) funded from Horizon 2020 [grant number 643071 ]. The authors would like to thank Á. Fernández (UDC, Spain) for her help and suggestions during the experiments. MCV and CK belong to the BIOENGIN group and thank the Xunta de Galicia for its financial support to Competitive Reference Research Groups (GRC) (ED431C 2017/66).

Funding Information:
This experimental work, undertaken at UDC (Spain), was financially supported by the Spanish Ministry of Economy, Industry and Competitiveness [project CTQ2017-88292-R] and the Marie Skłodowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET) funded from Horizon 2020 [grant number 643071]. The authors would like to thank Á. Fernández (UDC, Spain) for her help and suggestions during the experiments. MCV and CK belong to the BIOENGIN group and thank the Xunta de Galicia for its financial support to Competitive Reference Research Groups (GRC) (ED431C 2017/66).

Publisher Copyright:
© 2018 Elsevier Ltd

Funding

This experimental work, undertaken at UDC (Spain), was financially supported by the Spanish Ministry of Economy, Industry and Competitiveness [project CTQ2017-88292-R ] and the Marie Skłodowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET) funded from Horizon 2020 [grant number 643071 ]. The authors would like to thank Á. Fernández (UDC, Spain) for her help and suggestions during the experiments. MCV and CK belong to the BIOENGIN group and thank the Xunta de Galicia for its financial support to Competitive Reference Research Groups (GRC) (ED431C 2017/66). This experimental work, undertaken at UDC (Spain), was financially supported by the Spanish Ministry of Economy, Industry and Competitiveness [project CTQ2017-88292-R] and the Marie Skłodowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET) funded from Horizon 2020 [grant number 643071]. The authors would like to thank Á. Fernández (UDC, Spain) for her help and suggestions during the experiments. MCV and CK belong to the BIOENGIN group and thank the Xunta de Galicia for its financial support to Competitive Reference Research Groups (GRC) (ED431C 2017/66).

Keywords

Butanol
Ethanol
Hexanol
Solventogenesis
Volatile fatty acids

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10.1016/j.biortech.2018.10.002

SDU Link

Check access to the material in SDU Library's search engine

Advanced Biological waste to Energy technologies
Chakraborty, S. (Project participant)
01/06/2015 → 31/12/2018
Project: EU

Cite this

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title = "Enrichment of a solventogenic anaerobic sludge converting carbon monoxide and syngas into acids and alcohols",

abstract = "An anaerobic granular sludge was acclimatized to utilise CO in a continuously gas-fed stirred tank bioreactor by applying operating conditions expected to stimulate solventogenesis, i.e. the production of alcohols, and allowing to enrich for solventogenic populations. A cycle of high (6.2) and low (4.9) pH was applied in order to produce volatile fatty acids first at high pH, followed by their bioconversion into alcohols at low pH. The addition of yeast extract stimulated biomass growth, but not necessarily solventogenesis. The highest concentrations of metabolites achieved were 6.18 g/L acetic acid (30th day), 1.18 g/L butyric acid (28th day), and 0.423 g/L hexanoic acid (32nd day). Subsequently, acids were metabolized at lower pH, producing alcohols at concentrations of 11.1 g/L ethanol (43rd day), 1.8 g/L butanol (41st day) and 1.46 g/L hexanol (42nd day), confirming the successful enrichment strategy. Similarly, the enriched sludge could also convert syngas into acids and alcohols.",

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author = "Samayita Chakraborty and Rene, \{Eldon R.\} and Lens, \{Piet N.L.\} and Veiga, \{Mar{\'i}a C.\} and Christian Kennes",

note = "Funding Information: This experimental work, undertaken at UDC (Spain), was financially supported by the Spanish Ministry of Economy, Industry and Competitiveness [project CTQ2017-88292-R ] and the Marie Sk{\l}odowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET) funded from Horizon 2020 [grant number 643071 ]. The authors would like to thank {\'A}. Fern{\'a}ndez (UDC, Spain) for her help and suggestions during the experiments. MCV and CK belong to the BIOENGIN group and thank the Xunta de Galicia for its financial support to Competitive Reference Research Groups (GRC) (ED431C 2017/66). Funding Information: This experimental work, undertaken at UDC (Spain), was financially supported by the Spanish Ministry of Economy, Industry and Competitiveness [project CTQ2017-88292-R] and the Marie Sk{\l}odowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET) funded from Horizon 2020 [grant number 643071]. The authors would like to thank {\'A}. Fern{\'a}ndez (UDC, Spain) for her help and suggestions during the experiments. MCV and CK belong to the BIOENGIN group and thank the Xunta de Galicia for its financial support to Competitive Reference Research Groups (GRC) (ED431C 2017/66). Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2019",

month = jan,

doi = "10.1016/j.biortech.2018.10.002",

language = "English",

volume = "272",

pages = "130--136",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier",

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T1 - Enrichment of a solventogenic anaerobic sludge converting carbon monoxide and syngas into acids and alcohols

AU - Chakraborty, Samayita

AU - Rene, Eldon R.

AU - Lens, Piet N.L.

AU - Veiga, María C.

AU - Kennes, Christian

N1 - Funding Information: This experimental work, undertaken at UDC (Spain), was financially supported by the Spanish Ministry of Economy, Industry and Competitiveness [project CTQ2017-88292-R ] and the Marie Skłodowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET) funded from Horizon 2020 [grant number 643071 ]. The authors would like to thank Á. Fernández (UDC, Spain) for her help and suggestions during the experiments. MCV and CK belong to the BIOENGIN group and thank the Xunta de Galicia for its financial support to Competitive Reference Research Groups (GRC) (ED431C 2017/66). Funding Information: This experimental work, undertaken at UDC (Spain), was financially supported by the Spanish Ministry of Economy, Industry and Competitiveness [project CTQ2017-88292-R] and the Marie Skłodowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET) funded from Horizon 2020 [grant number 643071]. The authors would like to thank Á. Fernández (UDC, Spain) for her help and suggestions during the experiments. MCV and CK belong to the BIOENGIN group and thank the Xunta de Galicia for its financial support to Competitive Reference Research Groups (GRC) (ED431C 2017/66). Publisher Copyright: © 2018 Elsevier Ltd

PY - 2019/1

Y1 - 2019/1

N2 - An anaerobic granular sludge was acclimatized to utilise CO in a continuously gas-fed stirred tank bioreactor by applying operating conditions expected to stimulate solventogenesis, i.e. the production of alcohols, and allowing to enrich for solventogenic populations. A cycle of high (6.2) and low (4.9) pH was applied in order to produce volatile fatty acids first at high pH, followed by their bioconversion into alcohols at low pH. The addition of yeast extract stimulated biomass growth, but not necessarily solventogenesis. The highest concentrations of metabolites achieved were 6.18 g/L acetic acid (30th day), 1.18 g/L butyric acid (28th day), and 0.423 g/L hexanoic acid (32nd day). Subsequently, acids were metabolized at lower pH, producing alcohols at concentrations of 11.1 g/L ethanol (43rd day), 1.8 g/L butanol (41st day) and 1.46 g/L hexanol (42nd day), confirming the successful enrichment strategy. Similarly, the enriched sludge could also convert syngas into acids and alcohols.

AB - An anaerobic granular sludge was acclimatized to utilise CO in a continuously gas-fed stirred tank bioreactor by applying operating conditions expected to stimulate solventogenesis, i.e. the production of alcohols, and allowing to enrich for solventogenic populations. A cycle of high (6.2) and low (4.9) pH was applied in order to produce volatile fatty acids first at high pH, followed by their bioconversion into alcohols at low pH. The addition of yeast extract stimulated biomass growth, but not necessarily solventogenesis. The highest concentrations of metabolites achieved were 6.18 g/L acetic acid (30th day), 1.18 g/L butyric acid (28th day), and 0.423 g/L hexanoic acid (32nd day). Subsequently, acids were metabolized at lower pH, producing alcohols at concentrations of 11.1 g/L ethanol (43rd day), 1.8 g/L butanol (41st day) and 1.46 g/L hexanol (42nd day), confirming the successful enrichment strategy. Similarly, the enriched sludge could also convert syngas into acids and alcohols.

KW - Butanol

KW - Ethanol

KW - Hexanol

KW - Solventogenesis

KW - Volatile fatty acids

U2 - 10.1016/j.biortech.2018.10.002

DO - 10.1016/j.biortech.2018.10.002

M3 - Journal article

C2 - 30321830

AN - SCOPUS:85054657099

SN - 0960-8524

VL - 272

SP - 130

EP - 136

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Enrichment of a solventogenic anaerobic sludge converting carbon monoxide and syngas into acids and alcohols

Abstract

Bibliographical note

Funding

Keywords

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Advanced Biological waste to Energy technologies

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