@inbook{a236d0cf89e84fb5848a10f89d7c67e6,
title = "Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging",
abstract = "CRISPR-mediated endogenous tagging of genes provides unique possibilities to explore the function and dynamic subcellular localization of proteins in living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.",
keywords = "Cas12a, Cas9, Cilia, CRISPR, Fluorescently tagged proteins, Image analysis, Intraflagellar transport, Live-cell imaging, Vesicle trafficking, Cilia/metabolism, Proteins/metabolism, Humans, Biological Transport, Protein Transport/genetics",
author = "Stefanie Kuhns and Juhl, {Alice Dupont} and Zeinab Anvarian and Daniel W{\"u}stner and Pedersen, {Lotte B.} and Andersen, {Jens S.}",
note = "Funding Information: We thank Joel Pomerantz, Kazuhisa Nakayama, Michael Knop, Keith Joung, and Benjamin Kleinstiver for reagents and the Danish Molecular Biomedical Imaging Center, University of Southern Denmark, for use of imaging equipment, supported by Novo Nordisk Foundation (NNF18SA0032928). This work was supported by grants from Independent Research Fund Denmark (grant 8021-00425B) to J.S.A. and Novo Nordisk Foundation (NNF18OC0053024, NNF15OC0016886 and NNF14OC0011535), The Danish Cancer Society (R146-A9590) and Hartmann Foundation (A31662) to L.B.P. ",
year = "2024",
doi = "10.1007/978-1-0716-3507-0_9",
language = "English",
isbn = "978-1-0716-3509-4",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "147--166",
editor = "Vito Mennella",
booktitle = "Cilia",
address = "United States",
}