Efficient Management of High-Throughput Screening Libraries with SAVANAH

Markus List, Marlene Pedersen Elnegaard, Steffen Schmidt, Helle Christiansen, Qihua Tan, Jan Mollenhauer, Jan Baumbach

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

High-throughput screening (HTS) has become an indispensable tool for the pharmaceutical industry and for biomedical research. A high degree of automation allows for experiments in the range of a few hundred up to several hundred thousand to be performed in close succession. The basis for such screens are molecular libraries, that is, microtiter plates with solubilized reagents such as siRNAs, shRNAs, miRNA inhibitors or mimics, and sgRNAs, or small compounds, that is, drugs. These reagents are typically condensed to provide enough material for covering several screens. Library plates thus need to be serially diluted before they can be used as assay plates. This process, however, leads to an explosion in the number of plates and samples to be tracked. Here, we present SAVANAH, the first tool to effectively manage molecular screening libraries across dilution series. It conveniently links (connects) sample information from the library to experimental results from the assay plates. All results can be exported to the R statistical environment or piped into HiTSeekR (http://hitseekr.compbio.sdu.dk) for comprehensive follow-up analyses. In summary, SAVANAH supports the HTS community in managing and analyzing HTS experiments with an emphasis on serially diluted molecular libraries.
Original languageEnglish
JournalJournal of Biomolecular Screening
Volume22
Issue number2
Pages (from-to)196-202
ISSN1087-0571
DOIs
Publication statusPublished - 2017

Fingerprint

Libraries
Screening
Throughput
Assays
Explosions
MicroRNAs
Biomedical Research
Pharmaceutical Preparations
Dilution
Automation
Experiments
Industry

Cite this

List, Markus ; Elnegaard, Marlene Pedersen ; Schmidt, Steffen ; Christiansen, Helle ; Tan, Qihua ; Mollenhauer, Jan ; Baumbach, Jan. / Efficient Management of High-Throughput Screening Libraries with SAVANAH. In: Journal of Biomolecular Screening. 2017 ; Vol. 22, No. 2. pp. 196-202.
@article{b47e2e2ec47e461ca28dd8d64ce9ee59,
title = "Efficient Management of High-Throughput Screening Libraries with SAVANAH",
abstract = "High-throughput screening (HTS) has become an indispensable tool for the pharmaceutical industry and for biomedical research. A high degree of automation allows for experiments in the range of a few hundred up to several hundred thousand to be performed in close succession. The basis for such screens are molecular libraries, that is, microtiter plates with solubilized reagents such as siRNAs, shRNAs, miRNA inhibitors or mimics, and sgRNAs, or small compounds, that is, drugs. These reagents are typically condensed to provide enough material for covering several screens. Library plates thus need to be serially diluted before they can be used as assay plates. This process, however, leads to an explosion in the number of plates and samples to be tracked. Here, we present SAVANAH, the first tool to effectively manage molecular screening libraries across dilution series. It conveniently links (connects) sample information from the library to experimental results from the assay plates. All results can be exported to the R statistical environment or piped into HiTSeekR (http://hitseekr.compbio.sdu.dk) for comprehensive follow-up analyses. In summary, SAVANAH supports the HTS community in managing and analyzing HTS experiments with an emphasis on serially diluted molecular libraries.",
author = "Markus List and Elnegaard, {Marlene Pedersen} and Steffen Schmidt and Helle Christiansen and Qihua Tan and Jan Mollenhauer and Jan Baumbach",
note = "{\circledC} 2016 Society for Laboratory Automation and Screening.",
year = "2017",
doi = "10.1177/1087057116673607",
language = "English",
volume = "22",
pages = "196--202",
journal = "SLAS DISCOVERY: Advancing Life Sciences R&D",
issn = "2472-5552",
publisher = "SAGE Publications",
number = "2",

}

Efficient Management of High-Throughput Screening Libraries with SAVANAH. / List, Markus; Elnegaard, Marlene Pedersen; Schmidt, Steffen; Christiansen, Helle; Tan, Qihua; Mollenhauer, Jan; Baumbach, Jan.

In: Journal of Biomolecular Screening, Vol. 22, No. 2, 2017, p. 196-202.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Efficient Management of High-Throughput Screening Libraries with SAVANAH

AU - List, Markus

AU - Elnegaard, Marlene Pedersen

AU - Schmidt, Steffen

AU - Christiansen, Helle

AU - Tan, Qihua

AU - Mollenhauer, Jan

AU - Baumbach, Jan

N1 - © 2016 Society for Laboratory Automation and Screening.

PY - 2017

Y1 - 2017

N2 - High-throughput screening (HTS) has become an indispensable tool for the pharmaceutical industry and for biomedical research. A high degree of automation allows for experiments in the range of a few hundred up to several hundred thousand to be performed in close succession. The basis for such screens are molecular libraries, that is, microtiter plates with solubilized reagents such as siRNAs, shRNAs, miRNA inhibitors or mimics, and sgRNAs, or small compounds, that is, drugs. These reagents are typically condensed to provide enough material for covering several screens. Library plates thus need to be serially diluted before they can be used as assay plates. This process, however, leads to an explosion in the number of plates and samples to be tracked. Here, we present SAVANAH, the first tool to effectively manage molecular screening libraries across dilution series. It conveniently links (connects) sample information from the library to experimental results from the assay plates. All results can be exported to the R statistical environment or piped into HiTSeekR (http://hitseekr.compbio.sdu.dk) for comprehensive follow-up analyses. In summary, SAVANAH supports the HTS community in managing and analyzing HTS experiments with an emphasis on serially diluted molecular libraries.

AB - High-throughput screening (HTS) has become an indispensable tool for the pharmaceutical industry and for biomedical research. A high degree of automation allows for experiments in the range of a few hundred up to several hundred thousand to be performed in close succession. The basis for such screens are molecular libraries, that is, microtiter plates with solubilized reagents such as siRNAs, shRNAs, miRNA inhibitors or mimics, and sgRNAs, or small compounds, that is, drugs. These reagents are typically condensed to provide enough material for covering several screens. Library plates thus need to be serially diluted before they can be used as assay plates. This process, however, leads to an explosion in the number of plates and samples to be tracked. Here, we present SAVANAH, the first tool to effectively manage molecular screening libraries across dilution series. It conveniently links (connects) sample information from the library to experimental results from the assay plates. All results can be exported to the R statistical environment or piped into HiTSeekR (http://hitseekr.compbio.sdu.dk) for comprehensive follow-up analyses. In summary, SAVANAH supports the HTS community in managing and analyzing HTS experiments with an emphasis on serially diluted molecular libraries.

U2 - 10.1177/1087057116673607

DO - 10.1177/1087057116673607

M3 - Journal article

VL - 22

SP - 196

EP - 202

JO - SLAS DISCOVERY: Advancing Life Sciences R&D

JF - SLAS DISCOVERY: Advancing Life Sciences R&D

SN - 2472-5552

IS - 2

ER -