Effect of waterborne exposure to 4-tert-octylphenol and 17beta-estradiol on smoltification and downstream migration in Atlantic salmon, Salmo salar

Karsten Bangsgaard, Steffen S Madsen, Bodil Korsgaard

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Groups of Atlantic salmon parr (November, Exp. 1) or pre-smolts (March, Exp. 2) were exposed to estradiol-17beta (E2 conc.: nominal 500 ngl-1/actual 8-16 ngl-1) and two doses of tert-octylphenol (OP: nominal 25 µgl-1/actual 4.5-6.5 µgl-1 and OP: nominal 100 µgl-1/actual 10-30 µgl-1) for 26 days in fresh water, and the effects on physiological and behavioural aspects of parr-smolt transformation were investigated. Vitellogenesis was induced by all treatments, as indicated by elevated levels of plasma vitellogenin (Vtg) and hepatosomatic index. Elevated Vtg levels were still found in OP-100 and E2-treated fish 4-5 months after cessation of treatment, indicating a slow clearance of Vtg from circulation. Smolting was compromised by E2 and OP-100 treatment as judged by reduced gill Na+, K+-ATPase activity and impaired ability to regulate plasma osmolality and muscle water content in 24-h sea water (SW) challenge tests during the period of smolting. Downstream migratory behaviour was monitored from late April to July (Exp. 2) by implanting passive integrated transponder tags into subgroups of treated and control smolts and placing them in a stream raceway. Irrespective of treatment, nocturnal downstream movement was initiated in all groups on April 23, switching to diurnal movement in late May. Average swimming speed was estimated to be higher than current speed, indicating active migration. E2 and OP-100 fish migrated at lower frequency than control fish, suggesting a reduced migratory drive. The data suggests that waterborne exposure of salmon to xenoestrogens reduce both physiological and behavioural components of smoltification, even when exposure occurs several months prior to smolting.
Original languageEnglish
JournalAquatic Toxicology
Volume80
Issue number1
Pages (from-to)23-32
Number of pages9
ISSN0166-445X
DOIs
Publication statusPublished - 25. Oct 2006

Fingerprint

Vitellogenins
Salmo salar
smoltification
smolt
estradiol
vitellogenin
Fishes
smolts
parr
fish
Vitellogenesis
plasma
migratory behavior
Salmon
xenoestrogens
Seawater
Fresh Water
raceways
transponders
sodium-potassium-exchanging ATPase

Keywords

  • Salmon Migration
  • Environmental Exposure
  • Estradiol
  • Gills
  • Reproduction
  • Salmo salar
  • Vitellogenesis
  • Water Pollutants, Chemical

Cite this

@article{3e205ad0d96e11dc860c000ea68e967b,
title = "Effect of waterborne exposure to 4-tert-octylphenol and 17beta-estradiol on smoltification and downstream migration in Atlantic salmon, Salmo salar",
abstract = "Groups of Atlantic salmon parr (November, Exp. 1) or pre-smolts (March, Exp. 2) were exposed to estradiol-17beta (E2 conc.: nominal 500 ngl-1/actual 8-16 ngl-1) and two doses of tert-octylphenol (OP: nominal 25 µgl-1/actual 4.5-6.5 µgl-1 and OP: nominal 100 µgl-1/actual 10-30 µgl-1) for 26 days in fresh water, and the effects on physiological and behavioural aspects of parr-smolt transformation were investigated. Vitellogenesis was induced by all treatments, as indicated by elevated levels of plasma vitellogenin (Vtg) and hepatosomatic index. Elevated Vtg levels were still found in OP-100 and E2-treated fish 4-5 months after cessation of treatment, indicating a slow clearance of Vtg from circulation. Smolting was compromised by E2 and OP-100 treatment as judged by reduced gill Na+, K+-ATPase activity and impaired ability to regulate plasma osmolality and muscle water content in 24-h sea water (SW) challenge tests during the period of smolting. Downstream migratory behaviour was monitored from late April to July (Exp. 2) by implanting passive integrated transponder tags into subgroups of treated and control smolts and placing them in a stream raceway. Irrespective of treatment, nocturnal downstream movement was initiated in all groups on April 23, switching to diurnal movement in late May. Average swimming speed was estimated to be higher than current speed, indicating active migration. E2 and OP-100 fish migrated at lower frequency than control fish, suggesting a reduced migratory drive. The data suggests that waterborne exposure of salmon to xenoestrogens reduce both physiological and behavioural components of smoltification, even when exposure occurs several months prior to smolting.",
keywords = "Salmon Migration, Environmental Exposure, Estradiol, Gills, Reproduction, Salmo salar, Vitellogenesis, Water Pollutants, Chemical",
author = "Karsten Bangsgaard and Madsen, {Steffen S} and Bodil Korsgaard",
year = "2006",
month = "10",
day = "25",
doi = "10.1016/j.aquatox.2006.07.009",
language = "English",
volume = "80",
pages = "23--32",
journal = "Aquatic Toxicology",
issn = "0166-445X",
publisher = "Elsevier",
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Effect of waterborne exposure to 4-tert-octylphenol and 17beta-estradiol on smoltification and downstream migration in Atlantic salmon, Salmo salar. / Bangsgaard, Karsten; Madsen, Steffen S; Korsgaard, Bodil.

In: Aquatic Toxicology, Vol. 80, No. 1, 25.10.2006, p. 23-32.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Effect of waterborne exposure to 4-tert-octylphenol and 17beta-estradiol on smoltification and downstream migration in Atlantic salmon, Salmo salar

AU - Bangsgaard, Karsten

AU - Madsen, Steffen S

AU - Korsgaard, Bodil

PY - 2006/10/25

Y1 - 2006/10/25

N2 - Groups of Atlantic salmon parr (November, Exp. 1) or pre-smolts (March, Exp. 2) were exposed to estradiol-17beta (E2 conc.: nominal 500 ngl-1/actual 8-16 ngl-1) and two doses of tert-octylphenol (OP: nominal 25 µgl-1/actual 4.5-6.5 µgl-1 and OP: nominal 100 µgl-1/actual 10-30 µgl-1) for 26 days in fresh water, and the effects on physiological and behavioural aspects of parr-smolt transformation were investigated. Vitellogenesis was induced by all treatments, as indicated by elevated levels of plasma vitellogenin (Vtg) and hepatosomatic index. Elevated Vtg levels were still found in OP-100 and E2-treated fish 4-5 months after cessation of treatment, indicating a slow clearance of Vtg from circulation. Smolting was compromised by E2 and OP-100 treatment as judged by reduced gill Na+, K+-ATPase activity and impaired ability to regulate plasma osmolality and muscle water content in 24-h sea water (SW) challenge tests during the period of smolting. Downstream migratory behaviour was monitored from late April to July (Exp. 2) by implanting passive integrated transponder tags into subgroups of treated and control smolts and placing them in a stream raceway. Irrespective of treatment, nocturnal downstream movement was initiated in all groups on April 23, switching to diurnal movement in late May. Average swimming speed was estimated to be higher than current speed, indicating active migration. E2 and OP-100 fish migrated at lower frequency than control fish, suggesting a reduced migratory drive. The data suggests that waterborne exposure of salmon to xenoestrogens reduce both physiological and behavioural components of smoltification, even when exposure occurs several months prior to smolting.

AB - Groups of Atlantic salmon parr (November, Exp. 1) or pre-smolts (March, Exp. 2) were exposed to estradiol-17beta (E2 conc.: nominal 500 ngl-1/actual 8-16 ngl-1) and two doses of tert-octylphenol (OP: nominal 25 µgl-1/actual 4.5-6.5 µgl-1 and OP: nominal 100 µgl-1/actual 10-30 µgl-1) for 26 days in fresh water, and the effects on physiological and behavioural aspects of parr-smolt transformation were investigated. Vitellogenesis was induced by all treatments, as indicated by elevated levels of plasma vitellogenin (Vtg) and hepatosomatic index. Elevated Vtg levels were still found in OP-100 and E2-treated fish 4-5 months after cessation of treatment, indicating a slow clearance of Vtg from circulation. Smolting was compromised by E2 and OP-100 treatment as judged by reduced gill Na+, K+-ATPase activity and impaired ability to regulate plasma osmolality and muscle water content in 24-h sea water (SW) challenge tests during the period of smolting. Downstream migratory behaviour was monitored from late April to July (Exp. 2) by implanting passive integrated transponder tags into subgroups of treated and control smolts and placing them in a stream raceway. Irrespective of treatment, nocturnal downstream movement was initiated in all groups on April 23, switching to diurnal movement in late May. Average swimming speed was estimated to be higher than current speed, indicating active migration. E2 and OP-100 fish migrated at lower frequency than control fish, suggesting a reduced migratory drive. The data suggests that waterborne exposure of salmon to xenoestrogens reduce both physiological and behavioural components of smoltification, even when exposure occurs several months prior to smolting.

KW - Salmon Migration

KW - Environmental Exposure

KW - Estradiol

KW - Gills

KW - Reproduction

KW - Salmo salar

KW - Vitellogenesis

KW - Water Pollutants, Chemical

U2 - 10.1016/j.aquatox.2006.07.009

DO - 10.1016/j.aquatox.2006.07.009

M3 - Journal article

C2 - 16949683

VL - 80

SP - 23

EP - 32

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 0166-445X

IS - 1

ER -