Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast

S Mandrup; R Jepsen; H Skøtt; J Rosendal; P Højrup; K Kristiansen; J Knudsen

doi:10.1042/bj2900369

Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast

S Mandrup, R Jepsen, H Skøtt, J Rosendal, P Højrup, K Kristiansen, J Knudsen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.

Original language	English
Journal	Biochemical Journal
Volume	290
Issue number	2
Pages (from-to)	369-374
Number of pages	5
ISSN	0264-6021
DOIs	https://doi.org/10.1042/bj2900369
Publication status	Published - 1. Mar 1993

Keywords

Acyl Coenzyme A
Animals
Base Sequence
Carrier Proteins
Cattle
Cloning, Molecular
Escherichia coli
Fatty Acid-Binding Proteins
Molecular Sequence Data
Neoplasm Proteins
Oligonucleotides
Plasmids
Promoter Regions, Genetic
Recombinant Proteins
Saccharomyces cerevisiae

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10.1042/bj2900369

Cite this

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title = "Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast",

abstract = "We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.",

keywords = "Acyl Coenzyme A, Animals, Base Sequence, Carrier Proteins, Cattle, Cloning, Molecular, Escherichia coli, Fatty Acid-Binding Proteins, Molecular Sequence Data, Neoplasm Proteins, Oligonucleotides, Plasmids, Promoter Regions, Genetic, Recombinant Proteins, Saccharomyces cerevisiae",

author = "S Mandrup and R Jepsen and H Sk{\o}tt and J Rosendal and P H{\o}jrup and K Kristiansen and J Knudsen",

year = "1993",

month = mar,

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doi = "10.1042/bj2900369",

language = "English",

volume = "290",

pages = "369--374",

journal = "Biochemical Journal",

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TY - JOUR

T1 - Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast

AU - Mandrup, S

AU - Jepsen, R

AU - Skøtt, H

AU - Rosendal, J

AU - Højrup, P

AU - Kristiansen, K

AU - Knudsen, J

PY - 1993/3/1

Y1 - 1993/3/1

N2 - We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.

AB - We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.

KW - Acyl Coenzyme A

KW - Animals

KW - Base Sequence

KW - Carrier Proteins

KW - Cattle

KW - Cloning, Molecular

KW - Escherichia coli

KW - Fatty Acid-Binding Proteins

KW - Molecular Sequence Data

KW - Neoplasm Proteins

KW - Oligonucleotides

KW - Plasmids

KW - Promoter Regions, Genetic

KW - Recombinant Proteins

KW - Saccharomyces cerevisiae

U2 - 10.1042/bj2900369

DO - 10.1042/bj2900369

M3 - Journal article

C2 - 8452523

SN - 0264-6021

VL - 290

SP - 369

EP - 374

JO - Biochemical Journal

JF - Biochemical Journal

IS - 2

ER -

Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast

Abstract

Keywords

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