DNA methylation age and perceived age in elderly Danish twins

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Abstract

Perceived age is an easily accessible biomarker of aging. Here, we studied its relation to DNA methylation age (DNAm age) as introduced in (Horvath, 2013) in 180 elderly Danish twins. We found perceived age and DNAm age to be associated with chronological age (P = 0.04 resp. P = 2.2e-10) when correcting for gender, but did not see an association between perceived age and DNAm age (P = 0.44). Intrapair-analysis showed that the proportion of pairs where the twin with the highest perceived age also had the highest DNAm age was not different from 0.5 (P = 1), and we did not see a trend when dividing pairs according to their difference in perceived age (P = 0.36). Hence, intrapair analysis did not reveal links between perceived age and DNAm age. Moreover, none of the 353 CpGs underlying DNAm age was individually associated with perceived age after correction for multiple-testing (P > 6e-4, FDR > 0.21). Finally, when constructing an epigenetic signature based on these CpGs to predict perceived age, we only found a correlation of 0.18 (95%CI: −0.06 to 0.40) and a mean square error of 13.6 years 2 between observed and predicted values in the test dataset, indicating poor predictive strength. Altogether, our results suggest that perceived age and DNAm age capture different aging aspects.

Original languageEnglish
JournalMechanisms of Ageing and Development
Volume169
Pages (from-to)40-44
ISSN0047-6374
DOIs
Publication statusPublished - 1. Jan 2018

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DNA Methylation
Epigenomics

Keywords

  • Aged
  • Aged, 80 and over
  • Aging/blood
  • DNA Methylation
  • Denmark
  • Female
  • Humans
  • Male
  • Twins, Dizygotic
  • Twins, Monozygotic

Cite this

@article{520378c4009d400cb4d0ab4e11eeb746,
title = "DNA methylation age and perceived age in elderly Danish twins",
abstract = "Perceived age is an easily accessible biomarker of aging. Here, we studied its relation to DNA methylation age (DNAm age) as introduced in (Horvath, 2013) in 180 elderly Danish twins. We found perceived age and DNAm age to be associated with chronological age (P = 0.04 resp. P = 2.2e-10) when correcting for gender, but did not see an association between perceived age and DNAm age (P = 0.44). Intrapair-analysis showed that the proportion of pairs where the twin with the highest perceived age also had the highest DNAm age was not different from 0.5 (P = 1), and we did not see a trend when dividing pairs according to their difference in perceived age (P = 0.36). Hence, intrapair analysis did not reveal links between perceived age and DNAm age. Moreover, none of the 353 CpGs underlying DNAm age was individually associated with perceived age after correction for multiple-testing (P > 6e-4, FDR > 0.21). Finally, when constructing an epigenetic signature based on these CpGs to predict perceived age, we only found a correlation of 0.18 (95{\%}CI: −0.06 to 0.40) and a mean square error of 13.6 years 2 between observed and predicted values in the test dataset, indicating poor predictive strength. Altogether, our results suggest that perceived age and DNAm age capture different aging aspects.",
keywords = "Aged, Aged, 80 and over, Aging/blood, DNA Methylation, Denmark, Female, Humans, Male, Twins, Dizygotic, Twins, Monozygotic",
author = "Birgit Debrabant and Mette S{\o}rensen and Lene Christiansen and Qihua Tan and Matt McGue and Kaare Christensen and Hjelmborg, {Jacob v. B.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.mad.2017.09.004",
language = "English",
volume = "169",
pages = "40--44",
journal = "Mechanisms of Ageing and Development",
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TY - JOUR

T1 - DNA methylation age and perceived age in elderly Danish twins

AU - Debrabant, Birgit

AU - Sørensen, Mette

AU - Christiansen, Lene

AU - Tan, Qihua

AU - McGue, Matt

AU - Christensen, Kaare

AU - Hjelmborg, Jacob v. B.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Perceived age is an easily accessible biomarker of aging. Here, we studied its relation to DNA methylation age (DNAm age) as introduced in (Horvath, 2013) in 180 elderly Danish twins. We found perceived age and DNAm age to be associated with chronological age (P = 0.04 resp. P = 2.2e-10) when correcting for gender, but did not see an association between perceived age and DNAm age (P = 0.44). Intrapair-analysis showed that the proportion of pairs where the twin with the highest perceived age also had the highest DNAm age was not different from 0.5 (P = 1), and we did not see a trend when dividing pairs according to their difference in perceived age (P = 0.36). Hence, intrapair analysis did not reveal links between perceived age and DNAm age. Moreover, none of the 353 CpGs underlying DNAm age was individually associated with perceived age after correction for multiple-testing (P > 6e-4, FDR > 0.21). Finally, when constructing an epigenetic signature based on these CpGs to predict perceived age, we only found a correlation of 0.18 (95%CI: −0.06 to 0.40) and a mean square error of 13.6 years 2 between observed and predicted values in the test dataset, indicating poor predictive strength. Altogether, our results suggest that perceived age and DNAm age capture different aging aspects.

AB - Perceived age is an easily accessible biomarker of aging. Here, we studied its relation to DNA methylation age (DNAm age) as introduced in (Horvath, 2013) in 180 elderly Danish twins. We found perceived age and DNAm age to be associated with chronological age (P = 0.04 resp. P = 2.2e-10) when correcting for gender, but did not see an association between perceived age and DNAm age (P = 0.44). Intrapair-analysis showed that the proportion of pairs where the twin with the highest perceived age also had the highest DNAm age was not different from 0.5 (P = 1), and we did not see a trend when dividing pairs according to their difference in perceived age (P = 0.36). Hence, intrapair analysis did not reveal links between perceived age and DNAm age. Moreover, none of the 353 CpGs underlying DNAm age was individually associated with perceived age after correction for multiple-testing (P > 6e-4, FDR > 0.21). Finally, when constructing an epigenetic signature based on these CpGs to predict perceived age, we only found a correlation of 0.18 (95%CI: −0.06 to 0.40) and a mean square error of 13.6 years 2 between observed and predicted values in the test dataset, indicating poor predictive strength. Altogether, our results suggest that perceived age and DNAm age capture different aging aspects.

KW - Aged

KW - Aged, 80 and over

KW - Aging/blood

KW - DNA Methylation

KW - Denmark

KW - Female

KW - Humans

KW - Male

KW - Twins, Dizygotic

KW - Twins, Monozygotic

U2 - 10.1016/j.mad.2017.09.004

DO - 10.1016/j.mad.2017.09.004

M3 - Journal article

VL - 169

SP - 40

EP - 44

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

ER -