Distal middle cerebral artery occlusion does not result in depression-like behaviours

Yvonne Couch, Bettina Hjelm Clausen, Maria Ormhøj, Maria Gammelstrup Andersen, Christine Kring Nygaard, Maja Møller-Nielsen, Kate Lykke Lambertsen

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Background: Stroke is a devastating neurological injury, which can result in
significant cognitive and behavioural deficits. Modelling the disease processes
associated with stroke in animals is key to the development of novel therapeutic
approaches. However, some aspects of stroke pathophysiology, including
neuropsychiatric symptoms, do not translate well from humans to animals.
Here, we aimed to investigate the development of post-stroke depression in a
rodent model of stroke.
Methods: The distal middle cerebral artery (MCA) was permanently occluded
by electrocoagulation in adult male C57/Bl6/J mice. Animals were allowed to
survive for 6 hours, 24 hours, 2 days, 5 days or 7 days prior to behavioural
testing. Brains were taken to confirm lesion volumes at the above times.
Behavioural tests studied basic exploration and motivation (open field and
marble burying) as well as depression-like behaviours (tail suspension and
sucrose preference).
Results: Animals developed robust and reproducible lesions in the cortex but
whilst stroke reduced activity in the open field, animals showed no associated
behavioural deficits in any of the tests used for depression-like behaviours.
Conclusions: The distal middle cerebral artery occlusion (MCAO) model
produces a small cortical lesion which produces no depression-like behaviours.
These negative data are important for those wishing
Original languageEnglish
Article number1430
JournalF1000Research
Number of pages12
ISSN2046-1402
DOIs
Publication statusPublished - 7. Sep 2018

Cite this

Couch, Yvonne ; Clausen, Bettina Hjelm ; Ormhøj, Maria ; Andersen, Maria Gammelstrup ; Nygaard, Christine Kring ; Møller-Nielsen, Maja ; Lambertsen, Kate Lykke. / Distal middle cerebral artery occlusion does not result in depression-like behaviours. In: F1000Research. 2018.
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title = "Distal middle cerebral artery occlusion does not result in depression-like behaviours",
abstract = "Background: Stroke is a devastating neurological injury, which can result insignificant cognitive and behavioural deficits. Modelling the disease processesassociated with stroke in animals is key to the development of novel therapeuticapproaches. However, some aspects of stroke pathophysiology, includingneuropsychiatric symptoms, do not translate well from humans to animals.Here, we aimed to investigate the development of post-stroke depression in arodent model of stroke.Methods: The distal middle cerebral artery (MCA) was permanently occludedby electrocoagulation in adult male C57/Bl6/J mice. Animals were allowed tosurvive for 6 hours, 24 hours, 2 days, 5 days or 7 days prior to behaviouraltesting. Brains were taken to confirm lesion volumes at the above times.Behavioural tests studied basic exploration and motivation (open field andmarble burying) as well as depression-like behaviours (tail suspension andsucrose preference).Results: Animals developed robust and reproducible lesions in the cortex butwhilst stroke reduced activity in the open field, animals showed no associatedbehavioural deficits in any of the tests used for depression-like behaviours.Conclusions: The distal middle cerebral artery occlusion (MCAO) modelproduces a small cortical lesion which produces no depression-like behaviours.These negative data are important for those wishing",
author = "Yvonne Couch and Clausen, {Bettina Hjelm} and Maria Ormh{\o}j and Andersen, {Maria Gammelstrup} and Nygaard, {Christine Kring} and Maja M{\o}ller-Nielsen and Lambertsen, {Kate Lykke}",
year = "2018",
month = "9",
day = "7",
doi = "10.12688/f1000research.15769.1",
language = "English",
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Distal middle cerebral artery occlusion does not result in depression-like behaviours. / Couch, Yvonne; Clausen, Bettina Hjelm; Ormhøj, Maria; Andersen, Maria Gammelstrup; Nygaard, Christine Kring; Møller-Nielsen, Maja; Lambertsen, Kate Lykke.

In: F1000Research, 07.09.2018.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Distal middle cerebral artery occlusion does not result in depression-like behaviours

AU - Couch, Yvonne

AU - Clausen, Bettina Hjelm

AU - Ormhøj, Maria

AU - Andersen, Maria Gammelstrup

AU - Nygaard, Christine Kring

AU - Møller-Nielsen, Maja

AU - Lambertsen, Kate Lykke

PY - 2018/9/7

Y1 - 2018/9/7

N2 - Background: Stroke is a devastating neurological injury, which can result insignificant cognitive and behavioural deficits. Modelling the disease processesassociated with stroke in animals is key to the development of novel therapeuticapproaches. However, some aspects of stroke pathophysiology, includingneuropsychiatric symptoms, do not translate well from humans to animals.Here, we aimed to investigate the development of post-stroke depression in arodent model of stroke.Methods: The distal middle cerebral artery (MCA) was permanently occludedby electrocoagulation in adult male C57/Bl6/J mice. Animals were allowed tosurvive for 6 hours, 24 hours, 2 days, 5 days or 7 days prior to behaviouraltesting. Brains were taken to confirm lesion volumes at the above times.Behavioural tests studied basic exploration and motivation (open field andmarble burying) as well as depression-like behaviours (tail suspension andsucrose preference).Results: Animals developed robust and reproducible lesions in the cortex butwhilst stroke reduced activity in the open field, animals showed no associatedbehavioural deficits in any of the tests used for depression-like behaviours.Conclusions: The distal middle cerebral artery occlusion (MCAO) modelproduces a small cortical lesion which produces no depression-like behaviours.These negative data are important for those wishing

AB - Background: Stroke is a devastating neurological injury, which can result insignificant cognitive and behavioural deficits. Modelling the disease processesassociated with stroke in animals is key to the development of novel therapeuticapproaches. However, some aspects of stroke pathophysiology, includingneuropsychiatric symptoms, do not translate well from humans to animals.Here, we aimed to investigate the development of post-stroke depression in arodent model of stroke.Methods: The distal middle cerebral artery (MCA) was permanently occludedby electrocoagulation in adult male C57/Bl6/J mice. Animals were allowed tosurvive for 6 hours, 24 hours, 2 days, 5 days or 7 days prior to behaviouraltesting. Brains were taken to confirm lesion volumes at the above times.Behavioural tests studied basic exploration and motivation (open field andmarble burying) as well as depression-like behaviours (tail suspension andsucrose preference).Results: Animals developed robust and reproducible lesions in the cortex butwhilst stroke reduced activity in the open field, animals showed no associatedbehavioural deficits in any of the tests used for depression-like behaviours.Conclusions: The distal middle cerebral artery occlusion (MCAO) modelproduces a small cortical lesion which produces no depression-like behaviours.These negative data are important for those wishing

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