Different concentrations and volumes of p-phenylenediamine in pet. (equivalent doses) are associated with similar patch test outcomes: a pilot study

Flemming Andersen, Carsten R Hamann, Klaus E Andersen, Dathan Hamann, Eric Sager, Curtis Hamann*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

BACKGROUND: Concern about causing active sensitization when patch testing is performed with p-phenylenediamine (PPD) 1% pet. has led to a recommendation to use PPD 0.3% pet. as a potentially safer preparation. However, the dose per area of allergen delivered, and hence the risk of active sensitization, depend on the amount dispensed into the patch test chamber, which can vary widely.

OBJECTIVE: To evaluate whether patch testing with equivalent doses of different concentrations of PPD in pet. is associated with similar outcomes.

METHODS: Seventeen known PPD-sensitive subjects were patch tested with different volumes and concentrations of PPD in pet. that deliver the same allergen dose per unit area (6 mg of PPD 1% pet. and 20 mg of PPD 0.3% pet. in Finn Chambers®, both equivalent to ∼ 0.09 mg/cm2 ).

RESULTS: Eleven patients (65%) had positive reactions to both doses; 4 patients (24%) had negative results [percentage agreement of 88% (15/17)]. One patient each had a positive reaction to only one dose.

CONCLUSIONS: The 88% concordance suggests that dose per unit area is more important in determining reactions to allergens than the excipient volume dispensed. Patch testing with a smaller volume of 1% PPD may be a reasonable alternative to testing with 20 mg of 0.3% PPD.

Original languageEnglish
JournalContact Dermatitis
Volume78
Issue number5
Pages (from-to)335-340
ISSN0105-1873
DOIs
Publication statusPublished - May 2018

Keywords

  • Allergens/administration & dosage
  • Coloring Agents/administration & dosage
  • Dermatitis, Allergic Contact/diagnostic imaging
  • Dose-Response Relationship, Immunologic
  • Female
  • Humans
  • Male
  • Middle Aged
  • Patch Tests/adverse effects
  • Petrolatum
  • Phenylenediamines/administration & dosage
  • Pilot Projects
  • Prospective Studies

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