Diamond like carbon Ag nanocomposites as a control measure against Campylobacter jejuni and Listeria monocytogenes on food preparation surfaces

Sigitas Tamulevičius, Gintarė Zakarienė*, Aleksandr Novoslavskij, Šarūnas Meškinis, Andrius Vasiliauskas, Asta Tamulevičienė, Thomas Alter, Mindaugas Malakauskas

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The effect of thin (5 nm) and thick (40 nm) silver layers and diamond like carbon nanocomposites with embedded Ag nanoparticles (DLC:Ag) against two reference strains of C. jejuni NCTC 11168 and L. monocytogenes ATCC 7644 were evaluated in this study. DLC:Ag film contained 22 at.% Ag. Silver nanoparticle size measured by transmission electron microscope was in the 5–10 nm range. Ag layers and DLC:Ag nanocomposites were deposited employing unbalanced reactive magnetron sputtering on crystalline silicon wafers. C. jejuni and L. monocytogenes numbers were counted by culture-based enumeration on selective agars and quantitative real-time PCR (qPCR) including staining with propidium monoazide (PMA). It was determined, that DLC:Ag film was the most efficient coating in the reduction of C. jejuni and L. monocytogenes numbers. Culture-based enumeration revealed that C. jejuni numbers were reduced by an average of 4.06 log10CFU/ml after 15 min and 3.61 log10CFU/ml after 30 min on DLC:Ag coated silicon wafers in comparison to control samples (P ≤ 0.05). L. monocytogenes was not detected on DLC:Ag samples after 24 h of exposure (P ≤ 0.05). PMA-qPCR showed that C. jejuni and L. monocytogenes affected by DLC:Ag antimicrobial surface showed a reduced ability to grow on culture media, but maintained viability during the whole experiment. Nonetheless, DLC:Ag antimicrobial surface could be further considered for the reduction of cross-contamination risk from food preparation surfaces due to their contamination with C. jejuni and L. monocytogenes in domestic and commercial kitchens or food establishments.

Original languageEnglish
JournalDiamond and Related Materials
Volume81
Pages (from-to)118-126
ISSN0925-9635
DOIs
Publication statusPublished - 2018

Fingerprint

Listeria
Diamond
Diamonds
Nanocomposites
Carbon
Silicon wafers
Silver
Contamination
Nanoparticles
Kitchens
Reactive sputtering
Magnetron sputtering
Agar
Culture Media
Electron microscopes
Crystalline materials
Coatings
Experiments
propidium monoazide

Keywords

  • Campylobacter jejuni
  • DLC Ag nanocomposites
  • Listeria monocytogenes

Cite this

Tamulevičius, Sigitas ; Zakarienė, Gintarė ; Novoslavskij, Aleksandr ; Meškinis, Šarūnas ; Vasiliauskas, Andrius ; Tamulevičienė, Asta ; Alter, Thomas ; Malakauskas, Mindaugas. / Diamond like carbon Ag nanocomposites as a control measure against Campylobacter jejuni and Listeria monocytogenes on food preparation surfaces. In: Diamond and Related Materials. 2018 ; Vol. 81. pp. 118-126.
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abstract = "The effect of thin (5 nm) and thick (40 nm) silver layers and diamond like carbon nanocomposites with embedded Ag nanoparticles (DLC:Ag) against two reference strains of C. jejuni NCTC 11168 and L. monocytogenes ATCC 7644 were evaluated in this study. DLC:Ag film contained 22 at.{\%} Ag. Silver nanoparticle size measured by transmission electron microscope was in the 5–10 nm range. Ag layers and DLC:Ag nanocomposites were deposited employing unbalanced reactive magnetron sputtering on crystalline silicon wafers. C. jejuni and L. monocytogenes numbers were counted by culture-based enumeration on selective agars and quantitative real-time PCR (qPCR) including staining with propidium monoazide (PMA). It was determined, that DLC:Ag film was the most efficient coating in the reduction of C. jejuni and L. monocytogenes numbers. Culture-based enumeration revealed that C. jejuni numbers were reduced by an average of 4.06 log10CFU/ml after 15 min and 3.61 log10CFU/ml after 30 min on DLC:Ag coated silicon wafers in comparison to control samples (P ≤ 0.05). L. monocytogenes was not detected on DLC:Ag samples after 24 h of exposure (P ≤ 0.05). PMA-qPCR showed that C. jejuni and L. monocytogenes affected by DLC:Ag antimicrobial surface showed a reduced ability to grow on culture media, but maintained viability during the whole experiment. Nonetheless, DLC:Ag antimicrobial surface could be further considered for the reduction of cross-contamination risk from food preparation surfaces due to their contamination with C. jejuni and L. monocytogenes in domestic and commercial kitchens or food establishments.",
keywords = "Campylobacter jejuni, DLC Ag nanocomposites, Listeria monocytogenes",
author = "Sigitas Tamulevičius and Gintarė Zakarienė and Aleksandr Novoslavskij and Šarūnas Meškinis and Andrius Vasiliauskas and Asta Tamulevičienė and Thomas Alter and Mindaugas Malakauskas",
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doi = "10.1016/j.diamond.2017.12.007",
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Diamond like carbon Ag nanocomposites as a control measure against Campylobacter jejuni and Listeria monocytogenes on food preparation surfaces. / Tamulevičius, Sigitas; Zakarienė, Gintarė; Novoslavskij, Aleksandr; Meškinis, Šarūnas; Vasiliauskas, Andrius; Tamulevičienė, Asta; Alter, Thomas; Malakauskas, Mindaugas.

In: Diamond and Related Materials, Vol. 81, 2018, p. 118-126.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Diamond like carbon Ag nanocomposites as a control measure against Campylobacter jejuni and Listeria monocytogenes on food preparation surfaces

AU - Tamulevičius, Sigitas

AU - Zakarienė, Gintarė

AU - Novoslavskij, Aleksandr

AU - Meškinis, Šarūnas

AU - Vasiliauskas, Andrius

AU - Tamulevičienė, Asta

AU - Alter, Thomas

AU - Malakauskas, Mindaugas

PY - 2018

Y1 - 2018

N2 - The effect of thin (5 nm) and thick (40 nm) silver layers and diamond like carbon nanocomposites with embedded Ag nanoparticles (DLC:Ag) against two reference strains of C. jejuni NCTC 11168 and L. monocytogenes ATCC 7644 were evaluated in this study. DLC:Ag film contained 22 at.% Ag. Silver nanoparticle size measured by transmission electron microscope was in the 5–10 nm range. Ag layers and DLC:Ag nanocomposites were deposited employing unbalanced reactive magnetron sputtering on crystalline silicon wafers. C. jejuni and L. monocytogenes numbers were counted by culture-based enumeration on selective agars and quantitative real-time PCR (qPCR) including staining with propidium monoazide (PMA). It was determined, that DLC:Ag film was the most efficient coating in the reduction of C. jejuni and L. monocytogenes numbers. Culture-based enumeration revealed that C. jejuni numbers were reduced by an average of 4.06 log10CFU/ml after 15 min and 3.61 log10CFU/ml after 30 min on DLC:Ag coated silicon wafers in comparison to control samples (P ≤ 0.05). L. monocytogenes was not detected on DLC:Ag samples after 24 h of exposure (P ≤ 0.05). PMA-qPCR showed that C. jejuni and L. monocytogenes affected by DLC:Ag antimicrobial surface showed a reduced ability to grow on culture media, but maintained viability during the whole experiment. Nonetheless, DLC:Ag antimicrobial surface could be further considered for the reduction of cross-contamination risk from food preparation surfaces due to their contamination with C. jejuni and L. monocytogenes in domestic and commercial kitchens or food establishments.

AB - The effect of thin (5 nm) and thick (40 nm) silver layers and diamond like carbon nanocomposites with embedded Ag nanoparticles (DLC:Ag) against two reference strains of C. jejuni NCTC 11168 and L. monocytogenes ATCC 7644 were evaluated in this study. DLC:Ag film contained 22 at.% Ag. Silver nanoparticle size measured by transmission electron microscope was in the 5–10 nm range. Ag layers and DLC:Ag nanocomposites were deposited employing unbalanced reactive magnetron sputtering on crystalline silicon wafers. C. jejuni and L. monocytogenes numbers were counted by culture-based enumeration on selective agars and quantitative real-time PCR (qPCR) including staining with propidium monoazide (PMA). It was determined, that DLC:Ag film was the most efficient coating in the reduction of C. jejuni and L. monocytogenes numbers. Culture-based enumeration revealed that C. jejuni numbers were reduced by an average of 4.06 log10CFU/ml after 15 min and 3.61 log10CFU/ml after 30 min on DLC:Ag coated silicon wafers in comparison to control samples (P ≤ 0.05). L. monocytogenes was not detected on DLC:Ag samples after 24 h of exposure (P ≤ 0.05). PMA-qPCR showed that C. jejuni and L. monocytogenes affected by DLC:Ag antimicrobial surface showed a reduced ability to grow on culture media, but maintained viability during the whole experiment. Nonetheless, DLC:Ag antimicrobial surface could be further considered for the reduction of cross-contamination risk from food preparation surfaces due to their contamination with C. jejuni and L. monocytogenes in domestic and commercial kitchens or food establishments.

KW - Campylobacter jejuni

KW - DLC Ag nanocomposites

KW - Listeria monocytogenes

U2 - 10.1016/j.diamond.2017.12.007

DO - 10.1016/j.diamond.2017.12.007

M3 - Journal article

VL - 81

SP - 118

EP - 126

JO - Diamond and Related Materials

JF - Diamond and Related Materials

SN - 0925-9635

ER -