Development and validation of OECD test guidelines on mollusc reproductive toxicity tests

Laurent Lagadic, Henrik Holbech, Virginie Ducrot, INRA France

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearch

Abstract

Validated guidelines in line with the OECD Conceptual Framework for
the Testing and Assessment of Endocrine Disrupting Chemicals (EDTA)
have been developed for rodents, amphibians, fish, aquatic insects and
crustaceans. Only aquatic arthropods have been considered in this test
battery although the comparison of endpoints relevant for reproduction
in invertebrates often shows a much higher sensitivity in molluscs vs.
e.g. daphnids. The OECD test guideline programme has thus been
extended to cover reproduction effects of chemicals in molluscs.
Existing mollusc toxicity test protocols have been reviewed in an OECD
Detailed Review Paper that identifies two relevant candidate species for
developing freshwater tests: Potamopyrgus antipodarum and Lymnaea
stagnalis. However, this review did not clarify which toxicity test
design/conditions are the most appropriate for chemicals assessment.
Therefore, a mollusc reproduction test guideline will be developed
describing partial- and full- life-cycle test protocols in these species, so
as to propose a balanced suite of apical mollusc toxicity tests applicable
for the assessment of any type of chemical, including endocrine
disruptors, as level 4 and 5 assays of the EDTA Framework. The
guideline project is led by a consortium of experts (Germany/United-
Kingdom/France/Denmark) from academia, industry and government
stakeholders. To date, expert knowledge has been gathered and formed
the basis of draft standard operating procedures (SOPs) for the culture
and test implementation of the partial life-cycle tests in both species. Prevalidation
of these SOPs consisted in two ring-tests involving 10 partner
labs with different level of expertise in mollusc tests. Effects of
cadmium on the individual reproductive outputs were studied over 28-d
in P. antipodarum and over 56-d in L. stagnalis. Pre-validation tests
were successful in both species. Indeed, both species could be
successfully cultured at the lab. Toxicity tests provided consistent results
among laboratories for each species (e.g. homogenous and nonsignificantly
different NOECs, LOECs and EC
50
values for most
partner labs). The draft SOPs will be optimized (e.g. statistical tests
showed that the duration of the test with L. stagnalis can be shortened to
from 56-d to 35-d) and further tested for various types of reproductive
toxicants including endocrine disruptors. A broad scale ring-test will be
conducted in 2014 for both species based upon the consolidated SOPs.
Original languageEnglish
Publication date19. May 2013
Publication statusPublished - 19. May 2013
EventSETAC Europe 23rd meeting - Glasgow, United Kingdom
Duration: 12. May 201316. May 2013
Conference number: 23

Conference

ConferenceSETAC Europe 23rd meeting
Number23
CountryUnited Kingdom
CityGlasgow
Period12/05/201316/05/2013

Fingerprint

reproductive toxicology
toxicity testing
molluscs
standard operating procedures
endocrine-disrupting chemicals
testing
ring tests
Potamopyrgus antipodarum
life cycle (organisms)
aquatic arthropods
no observed adverse effect level
aquatic insects
expert opinion
endpoints
Denmark
United Kingdom
amphibians
reproductive performance
rodents
France

Cite this

Lagadic, L., Holbech, H., & Virginie Ducrot, INRA France (2013). Development and validation of OECD test guidelines on mollusc reproductive toxicity tests. Abstract from SETAC Europe 23rd meeting, Glasgow, United Kingdom.
Lagadic, Laurent ; Holbech, Henrik ; Virginie Ducrot, INRA France. / Development and validation of OECD test guidelines on mollusc reproductive toxicity tests. Abstract from SETAC Europe 23rd meeting, Glasgow, United Kingdom.
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Lagadic, L, Holbech, H & Virginie Ducrot, INRA France 2013, 'Development and validation of OECD test guidelines on mollusc reproductive toxicity tests' SETAC Europe 23rd meeting, Glasgow, United Kingdom, 12/05/2013 - 16/05/2013, .

Development and validation of OECD test guidelines on mollusc reproductive toxicity tests. / Lagadic, Laurent; Holbech, Henrik; Virginie Ducrot, INRA France.

2013. Abstract from SETAC Europe 23rd meeting, Glasgow, United Kingdom.

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearch

TY - ABST

T1 - Development and validation of OECD test guidelines on mollusc reproductive toxicity tests

AU - Lagadic, Laurent

AU - Holbech, Henrik

AU - hutchinson, tom

AU - Oehlmann, Jörg

AU - Virginie Ducrot, INRA France

PY - 2013/5/19

Y1 - 2013/5/19

N2 - Validated guidelines in line with the OECD Conceptual Framework forthe Testing and Assessment of Endocrine Disrupting Chemicals (EDTA)have been developed for rodents, amphibians, fish, aquatic insects andcrustaceans. Only aquatic arthropods have been considered in this testbattery although the comparison of endpoints relevant for reproductionin invertebrates often shows a much higher sensitivity in molluscs vs.e.g. daphnids. The OECD test guideline programme has thus beenextended to cover reproduction effects of chemicals in molluscs.Existing mollusc toxicity test protocols have been reviewed in an OECDDetailed Review Paper that identifies two relevant candidate species fordeveloping freshwater tests: Potamopyrgus antipodarum and Lymnaeastagnalis. However, this review did not clarify which toxicity testdesign/conditions are the most appropriate for chemicals assessment.Therefore, a mollusc reproduction test guideline will be developeddescribing partial- and full- life-cycle test protocols in these species, soas to propose a balanced suite of apical mollusc toxicity tests applicablefor the assessment of any type of chemical, including endocrinedisruptors, as level 4 and 5 assays of the EDTA Framework. Theguideline project is led by a consortium of experts (Germany/United-Kingdom/France/Denmark) from academia, industry and governmentstakeholders. To date, expert knowledge has been gathered and formedthe basis of draft standard operating procedures (SOPs) for the cultureand test implementation of the partial life-cycle tests in both species. Prevalidationof these SOPs consisted in two ring-tests involving 10 partnerlabs with different level of expertise in mollusc tests. Effects ofcadmium on the individual reproductive outputs were studied over 28-din P. antipodarum and over 56-d in L. stagnalis. Pre-validation testswere successful in both species. Indeed, both species could besuccessfully cultured at the lab. Toxicity tests provided consistent resultsamong laboratories for each species (e.g. homogenous and nonsignificantlydifferent NOECs, LOECs and EC50values for mostpartner labs). The draft SOPs will be optimized (e.g. statistical testsshowed that the duration of the test with L. stagnalis can be shortened tofrom 56-d to 35-d) and further tested for various types of reproductivetoxicants including endocrine disruptors. A broad scale ring-test will beconducted in 2014 for both species based upon the consolidated SOPs.

AB - Validated guidelines in line with the OECD Conceptual Framework forthe Testing and Assessment of Endocrine Disrupting Chemicals (EDTA)have been developed for rodents, amphibians, fish, aquatic insects andcrustaceans. Only aquatic arthropods have been considered in this testbattery although the comparison of endpoints relevant for reproductionin invertebrates often shows a much higher sensitivity in molluscs vs.e.g. daphnids. The OECD test guideline programme has thus beenextended to cover reproduction effects of chemicals in molluscs.Existing mollusc toxicity test protocols have been reviewed in an OECDDetailed Review Paper that identifies two relevant candidate species fordeveloping freshwater tests: Potamopyrgus antipodarum and Lymnaeastagnalis. However, this review did not clarify which toxicity testdesign/conditions are the most appropriate for chemicals assessment.Therefore, a mollusc reproduction test guideline will be developeddescribing partial- and full- life-cycle test protocols in these species, soas to propose a balanced suite of apical mollusc toxicity tests applicablefor the assessment of any type of chemical, including endocrinedisruptors, as level 4 and 5 assays of the EDTA Framework. Theguideline project is led by a consortium of experts (Germany/United-Kingdom/France/Denmark) from academia, industry and governmentstakeholders. To date, expert knowledge has been gathered and formedthe basis of draft standard operating procedures (SOPs) for the cultureand test implementation of the partial life-cycle tests in both species. Prevalidationof these SOPs consisted in two ring-tests involving 10 partnerlabs with different level of expertise in mollusc tests. Effects ofcadmium on the individual reproductive outputs were studied over 28-din P. antipodarum and over 56-d in L. stagnalis. Pre-validation testswere successful in both species. Indeed, both species could besuccessfully cultured at the lab. Toxicity tests provided consistent resultsamong laboratories for each species (e.g. homogenous and nonsignificantlydifferent NOECs, LOECs and EC50values for mostpartner labs). The draft SOPs will be optimized (e.g. statistical testsshowed that the duration of the test with L. stagnalis can be shortened tofrom 56-d to 35-d) and further tested for various types of reproductivetoxicants including endocrine disruptors. A broad scale ring-test will beconducted in 2014 for both species based upon the consolidated SOPs.

M3 - Conference abstract for conference

ER -

Lagadic L, Holbech H, Virginie Ducrot, INRA France. Development and validation of OECD test guidelines on mollusc reproductive toxicity tests. 2013. Abstract from SETAC Europe 23rd meeting, Glasgow, United Kingdom.