Determinants of house dust, endotoxin, and β-(1→3)-d-glucan in homes of Danish children

G Holst, A Høst, G Doekes, H W Meyer, A M Madsen, T Sigsgaard

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Little is known about the geographic variation and determinants of bacterial endotoxin and β -(1,3)-d-glucan in Danish house dust. In a population of 317 children, we: (i) described loads and concentrations of floor dust, endotoxin, and β-(1→3)-d-glucan and (ii) their correlations and (iii) assessed their determinants; (iv) Finally, we compared our findings with previous European studies. Bedroom floor dust was analyzed for endotoxin content by the kinetic limulus amoebocyte lysate assay and for β-(1→3)-d-glucan by the inhibition enzyme immunoassay. The parents answered questions regarding potential determinants. We found: geometric means (geometric standard deviations) 186 mg/m(2) (4.3) for dust; 5.46 × 10(3) EU/m(2) (8.0) and 31.1 × 10(3) EU/g (2.6) for endotoxin; and 142 μg/m(2) (14.3) and 0.71 × 10(3) μg/g (7.3) for β-(1→3)-d-glucan. High correlations (r > 0.75) were found between floor dust and endotoxin and β-(1→3)-d-glucan loads, while endotoxin and β-(1→3)-d-glucan concentrations were moderately correlated (r = 0.36-0.41) with the dust load. Having a carpet was positively associated with dust load and with endotoxin and β-(1→3)-d-glucan concentrations. Pet keeping, dwelling type, and dwelling location were determinants of endotoxin concentrations. No other determinants were associated with β-(1→3)-d-glucan concentrations. Compared with other European studies, we found lower β-(1→3)-d-glucan loads and concentrations but higher endotoxin loads and concentrations suggesting a geographically determined different composition of Danish floor dust compared with other European regions.

Original languageEnglish
JournalIndoor Air
Volume25
Issue number3
Pages (from-to)245–259
ISSN0905-6947
DOIs
Publication statusPublished - 2015

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Dust
Enzyme inhibition
Horseshoe Crabs
Assays
Parents
Kinetics
Chemical analysis
Population

Cite this

Holst, G., Høst, A., Doekes, G., Meyer, H. W., Madsen, A. M., & Sigsgaard, T. (2015). Determinants of house dust, endotoxin, and β-(1→3)-d-glucan in homes of Danish children. Indoor Air, 25(3), 245–259. https://doi.org/10.1111/ina.12143
Holst, G ; Høst, A ; Doekes, G ; Meyer, H W ; Madsen, A M ; Sigsgaard, T. / Determinants of house dust, endotoxin, and β-(1→3)-d-glucan in homes of Danish children. In: Indoor Air. 2015 ; Vol. 25, No. 3. pp. 245–259.
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Holst, G, Høst, A, Doekes, G, Meyer, HW, Madsen, AM & Sigsgaard, T 2015, 'Determinants of house dust, endotoxin, and β-(1→3)-d-glucan in homes of Danish children', Indoor Air, vol. 25, no. 3, pp. 245–259. https://doi.org/10.1111/ina.12143

Determinants of house dust, endotoxin, and β-(1→3)-d-glucan in homes of Danish children. / Holst, G; Høst, A; Doekes, G; Meyer, H W; Madsen, A M; Sigsgaard, T.

In: Indoor Air, Vol. 25, No. 3, 2015, p. 245–259.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Determinants of house dust, endotoxin, and β-(1→3)-d-glucan in homes of Danish children

AU - Holst, G

AU - Høst, A

AU - Doekes, G

AU - Meyer, H W

AU - Madsen, A M

AU - Sigsgaard, T

N1 - © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

PY - 2015

Y1 - 2015

N2 - Little is known about the geographic variation and determinants of bacterial endotoxin and β -(1,3)-d-glucan in Danish house dust. In a population of 317 children, we: (i) described loads and concentrations of floor dust, endotoxin, and β-(1→3)-d-glucan and (ii) their correlations and (iii) assessed their determinants; (iv) Finally, we compared our findings with previous European studies. Bedroom floor dust was analyzed for endotoxin content by the kinetic limulus amoebocyte lysate assay and for β-(1→3)-d-glucan by the inhibition enzyme immunoassay. The parents answered questions regarding potential determinants. We found: geometric means (geometric standard deviations) 186 mg/m(2) (4.3) for dust; 5.46 × 10(3) EU/m(2) (8.0) and 31.1 × 10(3) EU/g (2.6) for endotoxin; and 142 μg/m(2) (14.3) and 0.71 × 10(3) μg/g (7.3) for β-(1→3)-d-glucan. High correlations (r > 0.75) were found between floor dust and endotoxin and β-(1→3)-d-glucan loads, while endotoxin and β-(1→3)-d-glucan concentrations were moderately correlated (r = 0.36-0.41) with the dust load. Having a carpet was positively associated with dust load and with endotoxin and β-(1→3)-d-glucan concentrations. Pet keeping, dwelling type, and dwelling location were determinants of endotoxin concentrations. No other determinants were associated with β-(1→3)-d-glucan concentrations. Compared with other European studies, we found lower β-(1→3)-d-glucan loads and concentrations but higher endotoxin loads and concentrations suggesting a geographically determined different composition of Danish floor dust compared with other European regions.

AB - Little is known about the geographic variation and determinants of bacterial endotoxin and β -(1,3)-d-glucan in Danish house dust. In a population of 317 children, we: (i) described loads and concentrations of floor dust, endotoxin, and β-(1→3)-d-glucan and (ii) their correlations and (iii) assessed their determinants; (iv) Finally, we compared our findings with previous European studies. Bedroom floor dust was analyzed for endotoxin content by the kinetic limulus amoebocyte lysate assay and for β-(1→3)-d-glucan by the inhibition enzyme immunoassay. The parents answered questions regarding potential determinants. We found: geometric means (geometric standard deviations) 186 mg/m(2) (4.3) for dust; 5.46 × 10(3) EU/m(2) (8.0) and 31.1 × 10(3) EU/g (2.6) for endotoxin; and 142 μg/m(2) (14.3) and 0.71 × 10(3) μg/g (7.3) for β-(1→3)-d-glucan. High correlations (r > 0.75) were found between floor dust and endotoxin and β-(1→3)-d-glucan loads, while endotoxin and β-(1→3)-d-glucan concentrations were moderately correlated (r = 0.36-0.41) with the dust load. Having a carpet was positively associated with dust load and with endotoxin and β-(1→3)-d-glucan concentrations. Pet keeping, dwelling type, and dwelling location were determinants of endotoxin concentrations. No other determinants were associated with β-(1→3)-d-glucan concentrations. Compared with other European studies, we found lower β-(1→3)-d-glucan loads and concentrations but higher endotoxin loads and concentrations suggesting a geographically determined different composition of Danish floor dust compared with other European regions.

U2 - 10.1111/ina.12143

DO - 10.1111/ina.12143

M3 - Journal article

VL - 25

SP - 245

EP - 259

JO - Indoor Air

JF - Indoor Air

SN - 0905-6947

IS - 3

ER -