Detection of proteolytic signatures for Parkinson's disease

Peter Lüttge Jordal, Thomas Franck Dyrlund, Kristian Winge, Martin R. Larsen, Erik H Danielsen, James A. Wells, Daniel E. Otzen, Jan J. Enghild*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.

Original languageEnglish
JournalFuture Neurology
Volume11
Issue number1
Pages (from-to)15-32
ISSN1479-6708
DOIs
Publication statusPublished - 1. Feb 2016

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Keywords

  • cerebrospinal fluid
  • N-terminomics
  • Parkinson's disease

Cite this

Jordal, P. L., Dyrlund, T. F., Winge, K., Larsen, M. R., Danielsen, E. H., Wells, J. A., Otzen, D. E., & Enghild, J. J. (2016). Detection of proteolytic signatures for Parkinson's disease. Future Neurology, 11(1), 15-32. https://doi.org/10.2217/fnl.16.3