Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome

Morad Kamand; Mirolyuba Ilieva; Sheena Louise Forsberg; Mads Thomassen; Morten Meyer; Åsa Fex Svenningsen; Tanja Maria Michel

doi:10.1016/j.scr.2020.101974

Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome

Morad Kamand^*, Mirolyuba Ilieva, Sheena Louise Forsberg, Mads Thomassen, Morten Meyer, Åsa Fex Svenningsen, Tanja Maria Michel^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Autism spectrum disorder is a heterogenous neurodevelopmental disorder. The patients experience challenges in social interaction and communication skills as well as restricted and/or repetitive behaviors. To understand the molecular mechanisms underlying developmental brain disorders, patient-derived cellular models represent a useful tool. We have generated a human induced pluripotent stem cell line (SDUKIi003-A) from skin fibroblasts derived from a 20-year old male patient diagnosed with Asperger syndrome (“FYNEN-cohort” of Southern Denmark). The reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

Original language	English
Article number	101974
Journal	Stem Cell Research
Volume	48
Number of pages	5
ISSN	1873-5061
DOIs	https://doi.org/10.1016/j.scr.2020.101974
Publication status	Published - Oct 2020

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10.1016/j.scr.2020.101974Licence: CC BY-NC-ND

Open Access VersionFinal published version, 2.04 MBLicence: CC BY-NC-ND

1 Ph.D. thesis

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain
Kamand, M., 24. Jun 2022, Syddansk Universitet. Det Sundhedsvidenskabelige Fakultet. 225 p.
Research output: Thesis › Ph.D. thesis

Cite this

@article{4b543326b6af4e189df0cc507126508f,

title = "Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome",

abstract = "Autism spectrum disorder is a heterogenous neurodevelopmental disorder. The patients experience challenges in social interaction and communication skills as well as restricted and/or repetitive behaviors. To understand the molecular mechanisms underlying developmental brain disorders, patient-derived cellular models represent a useful tool. We have generated a human induced pluripotent stem cell line (SDUKIi003-A) from skin fibroblasts derived from a 20-year old male patient diagnosed with Asperger syndrome (“FYNEN-cohort” of Southern Denmark). The reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.",

author = "Morad Kamand and Mirolyuba Ilieva and {Louise Forsberg}, Sheena and Mads Thomassen and Morten Meyer and {Fex Svenningsen}, {\AA}sa and {Maria Michel}, Tanja",

year = "2020",

month = oct,

doi = "10.1016/j.scr.2020.101974",

language = "English",

volume = "48",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

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T1 - Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome

AU - Kamand, Morad

AU - Ilieva, Mirolyuba

AU - Louise Forsberg, Sheena

AU - Thomassen, Mads

AU - Meyer, Morten

AU - Fex Svenningsen, Åsa

AU - Maria Michel, Tanja

PY - 2020/10

Y1 - 2020/10

N2 - Autism spectrum disorder is a heterogenous neurodevelopmental disorder. The patients experience challenges in social interaction and communication skills as well as restricted and/or repetitive behaviors. To understand the molecular mechanisms underlying developmental brain disorders, patient-derived cellular models represent a useful tool. We have generated a human induced pluripotent stem cell line (SDUKIi003-A) from skin fibroblasts derived from a 20-year old male patient diagnosed with Asperger syndrome (“FYNEN-cohort” of Southern Denmark). The reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

AB - Autism spectrum disorder is a heterogenous neurodevelopmental disorder. The patients experience challenges in social interaction and communication skills as well as restricted and/or repetitive behaviors. To understand the molecular mechanisms underlying developmental brain disorders, patient-derived cellular models represent a useful tool. We have generated a human induced pluripotent stem cell line (SDUKIi003-A) from skin fibroblasts derived from a 20-year old male patient diagnosed with Asperger syndrome (“FYNEN-cohort” of Southern Denmark). The reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

U2 - 10.1016/j.scr.2020.101974

DO - 10.1016/j.scr.2020.101974

M3 - Journal article

C2 - 32916638

AN - SCOPUS:85090357129

SN - 1873-5061

VL - 48

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101974

ER -

Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome

Abstract

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Related research output

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain

Cite this