Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen

T Owens, M E Liddell, I N Crispe

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.
Original languageEnglish
JournalCellular Immunology
Volume85
Issue number2
Pages (from-to)436-46
Number of pages10
ISSN0008-8749
Publication statusPublished - 1984

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Blocking Antibodies
Clone Cells
Cross Reactions
Cell Line
Organism Cloning

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Surface
  • Autoantigens
  • Binding, Competitive
  • Cell Line
  • Clone Cells
  • Cross Reactions
  • H-2 Antigens
  • Haploidy
  • Histocompatibility Antigens Class II
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred A
  • Mice, Inbred AKR
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Inbred DBA
  • Minor Histocompatibility Loci
  • Phenotype
  • T-Lymphocytes

Cite this

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title = "Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen",
abstract = "An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.",
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author = "T Owens and Liddell, {M E} and Crispe, {I N}",
year = "1984",
language = "English",
volume = "85",
pages = "436--46",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Heinemann",
number = "2",

}

Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen. / Owens, T; Liddell, M E; Crispe, I N.

In: Cellular Immunology, Vol. 85, No. 2, 1984, p. 436-46.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen

AU - Owens, T

AU - Liddell, M E

AU - Crispe, I N

PY - 1984

Y1 - 1984

N2 - An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.

AB - An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.

KW - Animals

KW - Antibodies, Monoclonal

KW - Antigens, Surface

KW - Autoantigens

KW - Binding, Competitive

KW - Cell Line

KW - Clone Cells

KW - Cross Reactions

KW - H-2 Antigens

KW - Haploidy

KW - Histocompatibility Antigens Class II

KW - Lymphocyte Activation

KW - Mice

KW - Mice, Inbred A

KW - Mice, Inbred AKR

KW - Mice, Inbred BALB C

KW - Mice, Inbred C3H

KW - Mice, Inbred C57BL

KW - Mice, Inbred CBA

KW - Mice, Inbred DBA

KW - Minor Histocompatibility Loci

KW - Phenotype

KW - T-Lymphocytes

M3 - Journal article

C2 - 6609002

VL - 85

SP - 436

EP - 446

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 2

ER -