Abstract
| Original language | English |
|---|---|
| Publication date | 11. Nov 2014 |
| Publication status | Published - 11. Nov 2014 |
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Demographic parameters of individual E.coli within and among controlled environment. / Jouvet, Lionel; Steiner, Ulrich.
2014.Research output: Contribution to conference without publisher/journal › Conference abstract for conference › Communication
TY - ABST
T1 - Demographic parameters of individual E.coli within and among controlled environment
AU - Jouvet, Lionel
AU - Steiner, Ulrich
PY - 2014/11/11
Y1 - 2014/11/11
N2 - In most biodemographic studies, demographic parameters, such as lifespan and reproduction, are quantified from populations without controlling environmental or genetic influences. To accurately quantify the demographic parameters, we need to isolate the system from stochastic extrinsic factors. This can be achieved by working on isogenic populations under controlled environments. We use a microfluidic device to limit stochastic processes to their molecular components. The high throughput microfluidic device traps thousands of individual E. coli cells and tracks them over their lifespan. The devise allows a precise control of the culture media and temperature, i.e. the extrinsic environment. Preliminary results indicate substantial variation of lifespan and reproduction within the same environment, and significant shifts in mean lifespan and reproduction among environments. Variance in lifespan and reproduction decreases with increased temperature, as do the means. This study is setting the baseline observations on a range of temperature. This experimental setup opens a prolific research line, which will allow us to explore demographic effects of nutrients availability, molecular signals from bacteria’s host, and antibiotics on individual cells. Those individual level effects can then be scaled to the population level.
AB - In most biodemographic studies, demographic parameters, such as lifespan and reproduction, are quantified from populations without controlling environmental or genetic influences. To accurately quantify the demographic parameters, we need to isolate the system from stochastic extrinsic factors. This can be achieved by working on isogenic populations under controlled environments. We use a microfluidic device to limit stochastic processes to their molecular components. The high throughput microfluidic device traps thousands of individual E. coli cells and tracks them over their lifespan. The devise allows a precise control of the culture media and temperature, i.e. the extrinsic environment. Preliminary results indicate substantial variation of lifespan and reproduction within the same environment, and significant shifts in mean lifespan and reproduction among environments. Variance in lifespan and reproduction decreases with increased temperature, as do the means. This study is setting the baseline observations on a range of temperature. This experimental setup opens a prolific research line, which will allow us to explore demographic effects of nutrients availability, molecular signals from bacteria’s host, and antibiotics on individual cells. Those individual level effects can then be scaled to the population level.
M3 - Conference abstract for conference
ER -