Defining the diffusion in model membranes using line fluorescence recovery after photobleaching

Jakob L. Kure; Camilla B. Andersen; Thomas E. Rasmussen; B. Christoffer Lagerholm; Eva C. Arnspang

doi:10.3390/membranes10120434

Defining the diffusion in model membranes using line fluorescence recovery after photobleaching

Jakob L. Kure, Camilla B. Andersen, Thomas E. Rasmussen, B. Christoffer Lagerholm, Eva C. Arnspang^*

^*Corresponding author for this work

University of Oxford

Research output: Contribution to journal › Journal article › Research › peer-review

45 Downloads (Pure)

Abstract

In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging. The diffusion in two different synthetic membranes consisting of the lipid mixtures 1:1 DOPC:DPPC and 2:2:1 DOPC:DPPC:Cholesterol was studied with line FRAP. A correlation between diffusion coefficient and temperature was found to be dependent on the morphology of the membrane. We suggest line FRAP as a promising accessible and simple technique to study diffusion in plasma membranes.

Original language	English
Article number	434
Journal	Membranes
Volume	10
Issue number	12
Number of pages	7
ISSN	2077-0375
DOIs	https://doi.org/10.3390/membranes10120434
Publication status	Published - 17. Dec 2020

Keywords

Diffusion
Line FRAP
Microscopy
Plasma membrane lipids
Synthetic membrane

Documents & Links

10.3390/membranes10120434Licence: CC BY

Open Access VersionFinal published version, 984 KBLicence: CC BY

Cite this

@article{c9e06b9a3b114a049b2c842620a8b1b6,

title = "Defining the diffusion in model membranes using line fluorescence recovery after photobleaching",

abstract = "In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging. The diffusion in two different synthetic membranes consisting of the lipid mixtures 1:1 DOPC:DPPC and 2:2:1 DOPC:DPPC:Cholesterol was studied with line FRAP. A correlation between diffusion coefficient and temperature was found to be dependent on the morphology of the membrane. We suggest line FRAP as a promising accessible and simple technique to study diffusion in plasma membranes.",

keywords = "Diffusion, Line FRAP, Microscopy, Plasma membrane lipids, Synthetic membrane",

author = "Kure, {Jakob L.} and Andersen, {Camilla B.} and Rasmussen, {Thomas E.} and Lagerholm, {B. Christoffer} and Arnspang, {Eva C.}",

year = "2020",

month = dec,

day = "17",

doi = "10.3390/membranes10120434",

language = "English",

volume = "10",

journal = "Membranes",

issn = "2077-0375",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "12",

}

TY - JOUR

T1 - Defining the diffusion in model membranes using line fluorescence recovery after photobleaching

AU - Kure, Jakob L.

AU - Andersen, Camilla B.

AU - Rasmussen, Thomas E.

AU - Lagerholm, B. Christoffer

AU - Arnspang, Eva C.

PY - 2020/12/17

Y1 - 2020/12/17

N2 - In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging. The diffusion in two different synthetic membranes consisting of the lipid mixtures 1:1 DOPC:DPPC and 2:2:1 DOPC:DPPC:Cholesterol was studied with line FRAP. A correlation between diffusion coefficient and temperature was found to be dependent on the morphology of the membrane. We suggest line FRAP as a promising accessible and simple technique to study diffusion in plasma membranes.

AB - In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging. The diffusion in two different synthetic membranes consisting of the lipid mixtures 1:1 DOPC:DPPC and 2:2:1 DOPC:DPPC:Cholesterol was studied with line FRAP. A correlation between diffusion coefficient and temperature was found to be dependent on the morphology of the membrane. We suggest line FRAP as a promising accessible and simple technique to study diffusion in plasma membranes.

KW - Diffusion

KW - Line FRAP

KW - Microscopy

KW - Plasma membrane lipids

KW - Synthetic membrane

UR - https://www.mdpi.com/2077-0375/10/12/434

U2 - 10.3390/membranes10120434

DO - 10.3390/membranes10120434

M3 - Journal article

C2 - 33348780

AN - SCOPUS:85097828783

SN - 2077-0375

VL - 10

JO - Membranes

JF - Membranes

IS - 12

M1 - 434

ER -

Defining the diffusion in model membranes using line fluorescence recovery after photobleaching

Abstract

Keywords

Documents & Links

Other files and links

Fingerprint

Imaging Nano-domains in Mammalian Membranes Using Super-Resolution Microscopy and Advanced Analysis

Cite this

Defining the diffusion in model membranes using line fluorescence recovery after photobleaching

Abstract

Keywords

Documents & Links

Other files and links

Fingerprint

Related research output

Imaging Nano-domains in Mammalian Membranes Using Super-Resolution Microscopy and Advanced Analysis

Cite this