Decellularized matrix from tumorigenic human mesenchymal stem cells promotes neovascularization with galectin-1 dependent endothelial interaction

Jorge S Burns, Malthe Kristiansen, Lars P Kristensen, Kenneth H Larsen, Maria O Nielsen, Helle Christiansen, Jan Nehlin, Jens S. Andersen, Moustapha Kassem

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Acquisition of a blood supply is fundamental for extensive tumor growth. We recently described vascular heterogeneity in tumours derived from cell clones of a human mesenchymal stem cell (hMSC) strain (hMSC-TERT20) immortalized by retroviral vector mediated human telomerase (hTERT) gene expression. Histological analysis showed that cells of the most vascularized tumorigenic clone, -BD11 had a pericyte-like alpha smooth muscle actin (ASMA+) and CD146+ positive phenotype. Upon serum withdrawal in culture, -BD11 cells formed cord-like structures mimicking capillary morphogenesis. In contrast, cells of the poorly tumorigenic clone, -BC8 did not stain for ASMA, tumours were less vascularized and serum withdrawal in culture led to cell death. By exploring the heterogeneity in hMSC-TERT20 clones we aimed to understand molecular mechanisms by which mesenchymal stem cells may promote neovascularization.
Original languageEnglish
JournalP L o S One
Volume6
Issue number7
Pages (from-to)e21888
ISSN1932-6203
DOIs
Publication statusPublished - 1. Jan 2011

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