Decellularised human umbilical artery as a vascular graft elicits minimal pro-inflammatory host response ex vivo and in vivo

Alexander Høgsted Ahlmann, Shu Fang, Sussi Bagge Mortensen, Line Weis Andersen, Pernille Gejl Pedersen, Johanne Juel Callesen, Sara Thornby Bak, Kate Lykke Lambertsen, Ditte Caroline Andersen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Small diameter (<6 mm) vessel grafts still pose a challenge for scientists worldwide. De-cellularised umbilical artery (dUA) remains promising as small diameter tissue engineered vascular graft (TEVG), yet their immunogenicity remains unknown. Herein, we evaluated the host immune responses, with a focus on the innate part, towards human dUA implantation in mice, and con-firmed our findings in an ex vivo allogeneic human setup. Overall, we did not observe any differ-ences in the number of circulating white blood cells nor the number of monocytes among three groups of mice (1) dUA patch; (2) Sham; and (3) Mock throughout the study (day −7 to 28). Likewise, we found no difference in systemic inflammatory and anti‐inflammatory cytokine levels between groups. However, a massive local remodelling response with M2 macrophages were observed in the dUA at day 28, whereas M1 macrophages were less frequent. Moreover, human monocytes from allogeneic individuals were differentiated into macrophages and exposed to lyophilised dUA to maximize an eventual M1 response. Yet, dUA did not elicit any immediate M1 response as determined by the absence of CCR7 and CXCL10. Together this suggests that human dUA elicits a minimal pro‐inflammatory response further supporting its use as a TEVG in an allogeneic setup.

Original languageEnglish
Article number7981
JournalInternational Journal of Molecular Sciences
Volume22
Issue number15
Number of pages16
ISSN1661-6596
DOIs
Publication statusPublished - 1. Aug 2021

Keywords

  • Decellularisation
  • Human umbilical artery
  • Innate immunity
  • Macrophage M1 and M2 responses
  • Tissue engineered vascular grafting

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