Critical-depth Raman spectroscopy enables home-use non-invasive glucose monitoring

Signe M. Lundsgaard-Nielsen, Anders Pors, Stefan O. Banke, Jan E. Henriksen, Dietrich K. Hepp, Anders Weber*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

One of the most ambitious endeavors in the field of diabetes technology is non-invasive glucose sensing. In the past decades, a number of different technologies have been assessed, but none of these have found its entry into general clinical use. We report on the development of a table-top confocal Raman spectrometer that was used in the home of patients with diabetes and operated for extended periods of time unsupervised and without recalibration. The system is based on measurement of glucose levels at a ‘critical depth’ in the skin, specifically in the interstitial fluid located below the stratum corneum but above the underlying adipose tissue layer. The region chosen for routine glucose measurements was the base of the thumb (the thenar). In a small clinical study, 35 patients with diabetes analyzed their interstitial fluid glucose for a period of 60 days using the new critical-depth Raman (CD-Raman) method and levels were correlated to reference capillary blood glucose values using a standard finger-stick and test strip product. The calibration of the CD-Raman system was stable for > 10 days. Measurement performance for glucose levels present at, or below, a depth of ~250μm below the skin surface was comparable to that reported for currently available invasive continuous glucose monitors. In summary, using the CD-Raman technology we have demonstrated the first successful use of a non-invasive glucose monitor in the home.

Original languageEnglish
Article numbere0197134
JournalPLOS ONE
Volume13
Issue number5
Number of pages11
ISSN1932-6203
DOIs
Publication statusPublished - 2018

Fingerprint

Raman spectroscopy
Glucose
glucose
Monitoring
monitoring
Medical problems
diabetes
extracellular fluids
skin (animal)
Skin
Fluids
cornea
spectrometers
blood glucose
adipose tissue
Blood Glucose
Spectrometers
Adipose Tissue
clinical trials
calibration

Keywords

  • Adult
  • Blood Glucose Self-Monitoring/instrumentation
  • Blood Glucose/metabolism
  • Diabetes Mellitus/blood
  • Female
  • Humans
  • Male
  • Middle Aged
  • Spectrum Analysis, Raman/instrumentation

Cite this

Lundsgaard-Nielsen, S. M., Pors, A., Banke, S. O., Henriksen, J. E., Hepp, D. K., & Weber, A. (2018). Critical-depth Raman spectroscopy enables home-use non-invasive glucose monitoring. PLOS ONE, 13(5), [e0197134]. https://doi.org/10.1371/journal.pone.0197134
Lundsgaard-Nielsen, Signe M. ; Pors, Anders ; Banke, Stefan O. ; Henriksen, Jan E. ; Hepp, Dietrich K. ; Weber, Anders. / Critical-depth Raman spectroscopy enables home-use non-invasive glucose monitoring. In: PLOS ONE. 2018 ; Vol. 13, No. 5.
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Lundsgaard-Nielsen, SM, Pors, A, Banke, SO, Henriksen, JE, Hepp, DK & Weber, A 2018, 'Critical-depth Raman spectroscopy enables home-use non-invasive glucose monitoring', PLOS ONE, vol. 13, no. 5, e0197134. https://doi.org/10.1371/journal.pone.0197134

Critical-depth Raman spectroscopy enables home-use non-invasive glucose monitoring. / Lundsgaard-Nielsen, Signe M.; Pors, Anders; Banke, Stefan O.; Henriksen, Jan E.; Hepp, Dietrich K.; Weber, Anders.

In: PLOS ONE, Vol. 13, No. 5, e0197134, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Critical-depth Raman spectroscopy enables home-use non-invasive glucose monitoring

AU - Lundsgaard-Nielsen, Signe M.

AU - Pors, Anders

AU - Banke, Stefan O.

AU - Henriksen, Jan E.

AU - Hepp, Dietrich K.

AU - Weber, Anders

PY - 2018

Y1 - 2018

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AB - One of the most ambitious endeavors in the field of diabetes technology is non-invasive glucose sensing. In the past decades, a number of different technologies have been assessed, but none of these have found its entry into general clinical use. We report on the development of a table-top confocal Raman spectrometer that was used in the home of patients with diabetes and operated for extended periods of time unsupervised and without recalibration. The system is based on measurement of glucose levels at a ‘critical depth’ in the skin, specifically in the interstitial fluid located below the stratum corneum but above the underlying adipose tissue layer. The region chosen for routine glucose measurements was the base of the thumb (the thenar). In a small clinical study, 35 patients with diabetes analyzed their interstitial fluid glucose for a period of 60 days using the new critical-depth Raman (CD-Raman) method and levels were correlated to reference capillary blood glucose values using a standard finger-stick and test strip product. The calibration of the CD-Raman system was stable for > 10 days. Measurement performance for glucose levels present at, or below, a depth of ~250μm below the skin surface was comparable to that reported for currently available invasive continuous glucose monitors. In summary, using the CD-Raman technology we have demonstrated the first successful use of a non-invasive glucose monitor in the home.

KW - Adult

KW - Blood Glucose Self-Monitoring/instrumentation

KW - Blood Glucose/metabolism

KW - Diabetes Mellitus/blood

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Spectrum Analysis, Raman/instrumentation

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