TY - JOUR
T1 - Controls to validate plasma samples for cell free DNA quantification
AU - Pallisgaard, Niels
AU - Spindler, Karen-Lise Garm
AU - Andersen, Rikke Fredslund
AU - Brandslund, Ivan
AU - Jakobsen, Anders
N1 - Copyright © 2015 Elsevier B.V. All rights reserved.
PY - 2015/6/15
Y1 - 2015/6/15
N2 - Recent research has focused on the utility of cell free DNA (cfDNA) in serum and plasma for clinical application, especially in oncology. The literature holds promise of cfDNA as a valuable tumour marker to be used for treatment selection, monitoring and follow-up. The results, however, are diverging due to methodological differences with lack of standardisation and definition of sensitivity. The new biological information has not yet come into routine use. The present study presents external standardisation by spiking with non-human DNA fragments to control for loss of DNA during sample preparation and measurement. It also suggests a method to control for admixture of DNA from normal lymphocytes by utilizing the unique immunoglobulin gene rearrangement in the B-cells. The results show that this approach improves the quality of the analysis and lowers the risk of falsely increased values. In conclusion we suggest a new method to improve the accuracy of cfDNA measurements easily incorporated in the current technology.
AB - Recent research has focused on the utility of cell free DNA (cfDNA) in serum and plasma for clinical application, especially in oncology. The literature holds promise of cfDNA as a valuable tumour marker to be used for treatment selection, monitoring and follow-up. The results, however, are diverging due to methodological differences with lack of standardisation and definition of sensitivity. The new biological information has not yet come into routine use. The present study presents external standardisation by spiking with non-human DNA fragments to control for loss of DNA during sample preparation and measurement. It also suggests a method to control for admixture of DNA from normal lymphocytes by utilizing the unique immunoglobulin gene rearrangement in the B-cells. The results show that this approach improves the quality of the analysis and lowers the risk of falsely increased values. In conclusion we suggest a new method to improve the accuracy of cfDNA measurements easily incorporated in the current technology.
U2 - 10.1016/j.cca.2015.04.015
DO - 10.1016/j.cca.2015.04.015
M3 - Journal article
C2 - 25896958
SN - 0009-8981
VL - 446
SP - 141
EP - 146
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
ER -