Compartmentalized Aqueous–Organic Emulsion for Efficient Biocatalysis

Qingcai Zhao, Marion B. Ansorge-Schumacher, Rainer Haag, Changzhu Wu*

*Corresponding author for this work

Research output: Contribution to journalLetterResearchpeer-review

Abstract

The design and construction of polymeric compartmentalized structures in water have been intensively explored for controllable catalysis, but there is still the challenge of setting up catalytic compartments in organic media. Here, we designed a simple block copolymer, PCL-b-PEG-b-PCL, to construct a stable and multi-compartmentalized emulsion in an organic solvent by hand-shaking. This gentle emulsion preparation allowed a successful encapsulation of vulnerable biocatalysts such as benzaldehyde lyase (BAL) and alcohol dehydrogenase (ADH). The compartmentalization provided the emulsion with an exceptionally large interfacial area that could enhance BAL activity up to 225 times as compared to the traditional biphasic system. Moreover, the system could be easily scaled up due to its facile preparation with low cost. Therefore, our results pave the way for developing compartmentalized structures in solvents for biocatalysis in large-scale synthetic chemistry.

Original languageEnglish
JournalChemistry: A European Journal
Volume24
Issue number43
Pages (from-to)10966-10970
ISSN0947-6539
DOIs
Publication statusPublished - 1. Aug 2018

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Biocatalysis
Alcohol Dehydrogenase
Enzymes

Keywords

  • biocatalysis
  • block copolymer
  • compartmentalization
  • multiple emulsion
  • Biocatalysis
  • Emulsions/chemistry
  • Water/chemistry
  • Alcohol Dehydrogenase/metabolism
  • Fluorescent Dyes/chemistry
  • Polyesters/chemistry
  • Microscopy, Confocal
  • Polyethylene Glycols/chemistry
  • Aldehyde-Lyases/metabolism
  • Circular Dichroism

Cite this

Zhao, Qingcai ; Ansorge-Schumacher, Marion B. ; Haag, Rainer ; Wu, Changzhu. / Compartmentalized Aqueous–Organic Emulsion for Efficient Biocatalysis. In: Chemistry: A European Journal. 2018 ; Vol. 24, No. 43. pp. 10966-10970.
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abstract = "The design and construction of polymeric compartmentalized structures in water have been intensively explored for controllable catalysis, but there is still the challenge of setting up catalytic compartments in organic media. Here, we designed a simple block copolymer, PCL-b-PEG-b-PCL, to construct a stable and multi-compartmentalized emulsion in an organic solvent by hand-shaking. This gentle emulsion preparation allowed a successful encapsulation of vulnerable biocatalysts such as benzaldehyde lyase (BAL) and alcohol dehydrogenase (ADH). The compartmentalization provided the emulsion with an exceptionally large interfacial area that could enhance BAL activity up to 225 times as compared to the traditional biphasic system. Moreover, the system could be easily scaled up due to its facile preparation with low cost. Therefore, our results pave the way for developing compartmentalized structures in solvents for biocatalysis in large-scale synthetic chemistry.",
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Compartmentalized Aqueous–Organic Emulsion for Efficient Biocatalysis. / Zhao, Qingcai; Ansorge-Schumacher, Marion B.; Haag, Rainer; Wu, Changzhu.

In: Chemistry: A European Journal, Vol. 24, No. 43, 01.08.2018, p. 10966-10970.

Research output: Contribution to journalLetterResearchpeer-review

TY - JOUR

T1 - Compartmentalized Aqueous–Organic Emulsion for Efficient Biocatalysis

AU - Zhao, Qingcai

AU - Ansorge-Schumacher, Marion B.

AU - Haag, Rainer

AU - Wu, Changzhu

PY - 2018/8/1

Y1 - 2018/8/1

N2 - The design and construction of polymeric compartmentalized structures in water have been intensively explored for controllable catalysis, but there is still the challenge of setting up catalytic compartments in organic media. Here, we designed a simple block copolymer, PCL-b-PEG-b-PCL, to construct a stable and multi-compartmentalized emulsion in an organic solvent by hand-shaking. This gentle emulsion preparation allowed a successful encapsulation of vulnerable biocatalysts such as benzaldehyde lyase (BAL) and alcohol dehydrogenase (ADH). The compartmentalization provided the emulsion with an exceptionally large interfacial area that could enhance BAL activity up to 225 times as compared to the traditional biphasic system. Moreover, the system could be easily scaled up due to its facile preparation with low cost. Therefore, our results pave the way for developing compartmentalized structures in solvents for biocatalysis in large-scale synthetic chemistry.

AB - The design and construction of polymeric compartmentalized structures in water have been intensively explored for controllable catalysis, but there is still the challenge of setting up catalytic compartments in organic media. Here, we designed a simple block copolymer, PCL-b-PEG-b-PCL, to construct a stable and multi-compartmentalized emulsion in an organic solvent by hand-shaking. This gentle emulsion preparation allowed a successful encapsulation of vulnerable biocatalysts such as benzaldehyde lyase (BAL) and alcohol dehydrogenase (ADH). The compartmentalization provided the emulsion with an exceptionally large interfacial area that could enhance BAL activity up to 225 times as compared to the traditional biphasic system. Moreover, the system could be easily scaled up due to its facile preparation with low cost. Therefore, our results pave the way for developing compartmentalized structures in solvents for biocatalysis in large-scale synthetic chemistry.

KW - biocatalysis

KW - block copolymer

KW - compartmentalization

KW - multiple emulsion

KW - Biocatalysis

KW - Emulsions/chemistry

KW - Water/chemistry

KW - Alcohol Dehydrogenase/metabolism

KW - Fluorescent Dyes/chemistry

KW - Polyesters/chemistry

KW - Microscopy, Confocal

KW - Polyethylene Glycols/chemistry

KW - Aldehyde-Lyases/metabolism

KW - Circular Dichroism

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