Comparison between various biomarkers of senescence in bone marrow-derived stromal cells in vitro and ex-vivo

Jan Nehlin, Moustapha Kassem, Charles Frary

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

Abstract

Senescent stem cells are classified as non-quiescent, irreversibly growth-arrested, non-terminally differentiated, apoptosis resistant multipotent stem cells that maintain an altered gene expression from their juvenescent precursors. Established markers of senescence such as senescent-associated β-galactosidase, p16, and senescent-associated heterochromatic foci (SAHF) can only be analyzed through the use of cell toxic stains or fixatives while BOCS, biomarker of cellular senescence, along with certain morphological qualities can be visualized and quantified without inflicting any damage to cellular structures.
Bone marrow-derived stromal cells were isolated from young and old healthy subjects and cultured to senescence. The senescent cells were compared to their passage 1 counterparts through fluorescent high-throughput examination of C12FDG, SAHF, p16, BOCS stainings and morphology. This analysis was then repeated on passage 1 alone from both young and old healthy donors to examine the effect of donor age on biomarkers ex-vivo.
Cellular C12FDG staining, morphology, SAHF and nuclear p16 expression were increased similarly to BOCS from early to late passages. When bone marrow-derived stromal cells from young and old healthy subjects were compared ex-vivo, BOCS was the only biomarker found to be significantly up-regulated showing that BOCS correlates with the senescent phenotype at least as well as C12FDG and nuclear p16 stainings but without fixation or permeabilization.

Original languageEnglish
Publication date6. Sep 2013
Publication statusPublished - 6. Sep 2013
EventStress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle - Martin-Luther-University, Halle-Wittenberg, Germany, Denmark
Duration: 6. Sep 20138. Sep 2013

Conference

ConferenceStress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle
LocationMartin-Luther-University
CountryDenmark
CityHalle-Wittenberg, Germany
Period06/09/201308/09/2013

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Mesenchymal Stromal Cells
Galactosidases
Fixatives
Cell Aging
Poisons
Cellular Structures
Coloring Agents
Apoptosis
Growth
In Vitro Techniques

Cite this

Nehlin, J., Kassem, M., & Frary, C. (2013). Comparison between various biomarkers of senescence in bone marrow-derived stromal cells in vitro and ex-vivo. Abstract from Stress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle, Halle-Wittenberg, Germany, Denmark.
Nehlin, Jan ; Kassem, Moustapha ; Frary, Charles. / Comparison between various biomarkers of senescence in bone marrow-derived stromal cells in vitro and ex-vivo. Abstract from Stress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle, Halle-Wittenberg, Germany, Denmark.
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Nehlin, J, Kassem, M & Frary, C 2013, 'Comparison between various biomarkers of senescence in bone marrow-derived stromal cells in vitro and ex-vivo', Stress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle, Halle-Wittenberg, Germany, Denmark, 06/09/2013 - 08/09/2013.

Comparison between various biomarkers of senescence in bone marrow-derived stromal cells in vitro and ex-vivo. / Nehlin, Jan; Kassem, Moustapha; Frary, Charles.

2013. Abstract from Stress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle, Halle-Wittenberg, Germany, Denmark.

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

TY - ABST

T1 - Comparison between various biomarkers of senescence in bone marrow-derived stromal cells in vitro and ex-vivo

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N2 - Senescent stem cells are classified as non-quiescent, irreversibly growth-arrested, non-terminally differentiated, apoptosis resistant multipotent stem cells that maintain an altered gene expression from their juvenescent precursors. Established markers of senescence such as senescent-associated β-galactosidase, p16, and senescent-associated heterochromatic foci (SAHF) can only be analyzed through the use of cell toxic stains or fixatives while BOCS, biomarker of cellular senescence, along with certain morphological qualities can be visualized and quantified without inflicting any damage to cellular structures. Bone marrow-derived stromal cells were isolated from young and old healthy subjects and cultured to senescence. The senescent cells were compared to their passage 1 counterparts through fluorescent high-throughput examination of C12FDG, SAHF, p16, BOCS stainings and morphology. This analysis was then repeated on passage 1 alone from both young and old healthy donors to examine the effect of donor age on biomarkers ex-vivo. Cellular C12FDG staining, morphology, SAHF and nuclear p16 expression were increased similarly to BOCS from early to late passages. When bone marrow-derived stromal cells from young and old healthy subjects were compared ex-vivo, BOCS was the only biomarker found to be significantly up-regulated showing that BOCS correlates with the senescent phenotype at least as well as C12FDG and nuclear p16 stainings but without fixation or permeabilization.

AB - Senescent stem cells are classified as non-quiescent, irreversibly growth-arrested, non-terminally differentiated, apoptosis resistant multipotent stem cells that maintain an altered gene expression from their juvenescent precursors. Established markers of senescence such as senescent-associated β-galactosidase, p16, and senescent-associated heterochromatic foci (SAHF) can only be analyzed through the use of cell toxic stains or fixatives while BOCS, biomarker of cellular senescence, along with certain morphological qualities can be visualized and quantified without inflicting any damage to cellular structures. Bone marrow-derived stromal cells were isolated from young and old healthy subjects and cultured to senescence. The senescent cells were compared to their passage 1 counterparts through fluorescent high-throughput examination of C12FDG, SAHF, p16, BOCS stainings and morphology. This analysis was then repeated on passage 1 alone from both young and old healthy donors to examine the effect of donor age on biomarkers ex-vivo. Cellular C12FDG staining, morphology, SAHF and nuclear p16 expression were increased similarly to BOCS from early to late passages. When bone marrow-derived stromal cells from young and old healthy subjects were compared ex-vivo, BOCS was the only biomarker found to be significantly up-regulated showing that BOCS correlates with the senescent phenotype at least as well as C12FDG and nuclear p16 stainings but without fixation or permeabilization.

M3 - Conference abstract for conference

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Nehlin J, Kassem M, Frary C. Comparison between various biomarkers of senescence in bone marrow-derived stromal cells in vitro and ex-vivo. 2013. Abstract from Stress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle, Halle-Wittenberg, Germany, Denmark.