Combining microfluidics, optogenetics and calcium imaging to study neuronal communication in vitro

Renaud Renault, Nirit Sukenik, Stéphanie Descroix, Laurent Malaquin, Jean Louis Viovy, Jean Michel Peyrin, Samuel Bottani, Pascal Monceau, Elisha Moses, Maéva Vignes

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In this paper we report the combination of microfluidics, optogenetics and calcium imaging as a cheap and convenient platform to study synaptic communication between neuronal populations in vitro. We first show that Calcium Orange indicator is compatible in vitro with a commonly used Channelrhodopsine-2 (ChR2) variant, as standard calcium imaging conditions did not alter significantly the activity of transduced cultures of rodent primary neurons. A fast, robust and scalable process for micro-chip fabrication was developed in parallel to build micro-compartmented cultures. Coupling optical fibers to each micro-compartment allowed for the independent control of ChR2 activation in the different populations without crosstalk. By analyzing the post-stimuli activity across the different populations, we finally show how this platform can be used to evaluate quantitatively the effective connectivity between connected neuronal populations.

Original languageEnglish
Article numbere0120680
JournalPLOS ONE
Volume10
Issue number4
Number of pages15
ISSN1932-6203
DOIs
Publication statusPublished - 2015
Externally publishedYes

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