Colorimetric determination of orthophosphate in the assay of inorganic pyrophosphatase activity

Mogens Hørder*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Using Baginski and Zak's (12) method for determining inorganic orthophosphate as a starting point, a number of conditions which influence the accuracy and precision of the determination of pyrophosphatase activity have been shown: nonenzyme-catalyzed acid hydrolysis by protein precipitation agents and molybdate-catalyzed hydrolysis of pyrophosphate together with interference in the determination of orthophosphate by the substrate pyrophosphate and other components from the reaction mixture, Tris, chloride, acetate, citrate, boric acid. With regard to these sources of error, a method is described for determining pyrophosphatase activity, and its reliability is investigated.

Original languageEnglish
JournalAnalytical Biochemistry
Volume49
Issue number1
Pages (from-to)37-47
ISSN0003-2697
DOIs
Publication statusPublished - 1. Jan 1972
Externally publishedYes

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