Staphylococcus aureus is a major human pathogen and known for causing vascular infections such as sepsis and infective endocarditis. It has previously been proposed that S. aureus succeed in colonization of the endothelial wall by specific surface attachment likely followed by biofilm formation. Furthermore, S. aureus is known to invade human cells, which has been proposed to promote persistence through immune and antibiotic evasion. In the current study, we sought to investigate endothelial colonization, invasion, and biofilm formation by S. aureus using a newly developed in vitro flow chamber model. We show that under physiological shear rates, S. aureus utilizes cellular invasion to enable the following surface colonization and biofilm formation. These observations might help explain the success of S. aureus as a bloodstream pathogen and guide further studies in S. aureus pathogenesis and treatment of S. aureus biofilms.
|Publication date||28. May 2018|
|Number of pages||1|
|Publication status||Published - 28. May 2018|
|Event||Biofilms8 - Aarhus University, Aarhus, Denmark|
Duration: 27. May 2018 → 29. May 2018
|Period||27/05/2018 → 29/05/2018|
- Staphylococcus aureus
Grønnemose, R. B., Antoinette Asferg, C., Kolmos, H. J., & Andersen, T. E. (2018). Colonization and biofilm formation by Staphylococcus aureus on endothelial cell layers under flow. Poster session presented at Biofilms8, Aarhus, Denmark.