CL-L1 and CL-K1 Exhibit Widespread Tissue Distribution With High and Co-Localized Expression in Secretory Epithelia and Mucosa

Soren W K Hansen*, Josephine B Aagaard, Karen B Bjerrum, Eva K Hejbøl, Ole Nielsen, Henrik D Schrøder, Karsten Skjoedt, Anna L Sørensen, Jonas H Graversen, Maiken L Henriksen

*Corresponding author for this work

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Abstract

Collectin liver 1 (CL-L1, alias collectin 10) and collectin kidney 1 (CL-K1, alias collectin 11) are oligomeric pattern recognition molecules associated with the complement system, and mutations in either of their genes may lead to deficiency and developmental defects. The two collectins are reportedly localized and synthesized in the liver, kidneys, and adrenals, and can be found in the circulation as heteromeric complexes (CL-LK), which upon binding to microbial high mannose-like glycoconjugates activates the complement system via the lectin activation pathway. The tissue distribution of homo- vs. heteromeric CL-L1 and -K1 complexes, the mechanism of heteromeric complex formation and in which tissues this occurs, is hitherto incompletely described. We have by immunohistochemistry using monoclonal antibodies addressed the precise cellular localization of the two collectins in the main human tissues. We find that the two collectins have widespread and almost identical tissue distribution with a high expression in epithelial cells in endo-/exocrine secretory tissues and mucosa. There is also accordance between localization of mRNA transcripts and detection of proteins, showing that local synthesis likely is responsible for peripheral localization and eventual formation of the CL-LK complexes. The functional implications of the high expression in endo-/exocrine secretory tissue and mucosa is unknown but might be associated with the activity of MASP-3, which has a similar pattern of expression and is known to potentiate the activity of the alternative complement activation pathway.

Original languageEnglish
Article number1757
JournalFrontiers in Immunology
Volume9
Number of pages12
ISSN1664-3224
DOIs
Publication statusPublished - 2018

Fingerprint

Mucous Membrane
Epithelium
Mannose-Binding Protein-Associated Serine Proteases
Alternative Complement Pathway
Kidney
Liver
Mannose
Epithelial Cells
Messenger RNA
Mutation
Proteins

Keywords

  • 3MC syndrome
  • collectin
  • complement system
  • innate immunity
  • mucosal immunology

Cite this

@article{056788eb973f404d9dc0ebd30f2bb2e4,
title = "CL-L1 and CL-K1 Exhibit Widespread Tissue Distribution With High and Co-Localized Expression in Secretory Epithelia and Mucosa",
abstract = "Collectin liver 1 (CL-L1, alias collectin 10) and collectin kidney 1 (CL-K1, alias collectin 11) are oligomeric pattern recognition molecules associated with the complement system, and mutations in either of their genes may lead to deficiency and developmental defects. The two collectins are reportedly localized and synthesized in the liver, kidneys, and adrenals, and can be found in the circulation as heteromeric complexes (CL-LK), which upon binding to microbial high mannose-like glycoconjugates activates the complement system via the lectin activation pathway. The tissue distribution of homo- vs. heteromeric CL-L1 and -K1 complexes, the mechanism of heteromeric complex formation and in which tissues this occurs, is hitherto incompletely described. We have by immunohistochemistry using monoclonal antibodies addressed the precise cellular localization of the two collectins in the main human tissues. We find that the two collectins have widespread and almost identical tissue distribution with a high expression in epithelial cells in endo-/exocrine secretory tissues and mucosa. There is also accordance between localization of mRNA transcripts and detection of proteins, showing that local synthesis likely is responsible for peripheral localization and eventual formation of the CL-LK complexes. The functional implications of the high expression in endo-/exocrine secretory tissue and mucosa is unknown but might be associated with the activity of MASP-3, which has a similar pattern of expression and is known to potentiate the activity of the alternative complement activation pathway.",
keywords = "3MC syndrome, collectin, complement system, innate immunity, mucosal immunology",
author = "Hansen, {Soren W K} and Aagaard, {Josephine B} and Bjerrum, {Karen B} and Hejb{\o}l, {Eva K} and Ole Nielsen and Schr{\o}der, {Henrik D} and Karsten Skjoedt and S{\o}rensen, {Anna L} and Graversen, {Jonas H} and Henriksen, {Maiken L}",
year = "2018",
doi = "10.3389/fimmu.2018.01757",
language = "English",
volume = "9",
journal = "Frontiers in Immunology",
issn = "1664-3224",
publisher = "Frontiers Research Foundation",

}

CL-L1 and CL-K1 Exhibit Widespread Tissue Distribution With High and Co-Localized Expression in Secretory Epithelia and Mucosa. / Hansen, Soren W K; Aagaard, Josephine B; Bjerrum, Karen B; Hejbøl, Eva K; Nielsen, Ole; Schrøder, Henrik D; Skjoedt, Karsten; Sørensen, Anna L; Graversen, Jonas H; Henriksen, Maiken L.

In: Frontiers in Immunology, Vol. 9, 1757, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - CL-L1 and CL-K1 Exhibit Widespread Tissue Distribution With High and Co-Localized Expression in Secretory Epithelia and Mucosa

AU - Hansen, Soren W K

AU - Aagaard, Josephine B

AU - Bjerrum, Karen B

AU - Hejbøl, Eva K

AU - Nielsen, Ole

AU - Schrøder, Henrik D

AU - Skjoedt, Karsten

AU - Sørensen, Anna L

AU - Graversen, Jonas H

AU - Henriksen, Maiken L

PY - 2018

Y1 - 2018

N2 - Collectin liver 1 (CL-L1, alias collectin 10) and collectin kidney 1 (CL-K1, alias collectin 11) are oligomeric pattern recognition molecules associated with the complement system, and mutations in either of their genes may lead to deficiency and developmental defects. The two collectins are reportedly localized and synthesized in the liver, kidneys, and adrenals, and can be found in the circulation as heteromeric complexes (CL-LK), which upon binding to microbial high mannose-like glycoconjugates activates the complement system via the lectin activation pathway. The tissue distribution of homo- vs. heteromeric CL-L1 and -K1 complexes, the mechanism of heteromeric complex formation and in which tissues this occurs, is hitherto incompletely described. We have by immunohistochemistry using monoclonal antibodies addressed the precise cellular localization of the two collectins in the main human tissues. We find that the two collectins have widespread and almost identical tissue distribution with a high expression in epithelial cells in endo-/exocrine secretory tissues and mucosa. There is also accordance between localization of mRNA transcripts and detection of proteins, showing that local synthesis likely is responsible for peripheral localization and eventual formation of the CL-LK complexes. The functional implications of the high expression in endo-/exocrine secretory tissue and mucosa is unknown but might be associated with the activity of MASP-3, which has a similar pattern of expression and is known to potentiate the activity of the alternative complement activation pathway.

AB - Collectin liver 1 (CL-L1, alias collectin 10) and collectin kidney 1 (CL-K1, alias collectin 11) are oligomeric pattern recognition molecules associated with the complement system, and mutations in either of their genes may lead to deficiency and developmental defects. The two collectins are reportedly localized and synthesized in the liver, kidneys, and adrenals, and can be found in the circulation as heteromeric complexes (CL-LK), which upon binding to microbial high mannose-like glycoconjugates activates the complement system via the lectin activation pathway. The tissue distribution of homo- vs. heteromeric CL-L1 and -K1 complexes, the mechanism of heteromeric complex formation and in which tissues this occurs, is hitherto incompletely described. We have by immunohistochemistry using monoclonal antibodies addressed the precise cellular localization of the two collectins in the main human tissues. We find that the two collectins have widespread and almost identical tissue distribution with a high expression in epithelial cells in endo-/exocrine secretory tissues and mucosa. There is also accordance between localization of mRNA transcripts and detection of proteins, showing that local synthesis likely is responsible for peripheral localization and eventual formation of the CL-LK complexes. The functional implications of the high expression in endo-/exocrine secretory tissue and mucosa is unknown but might be associated with the activity of MASP-3, which has a similar pattern of expression and is known to potentiate the activity of the alternative complement activation pathway.

KW - 3MC syndrome

KW - collectin

KW - complement system

KW - innate immunity

KW - mucosal immunology

U2 - 10.3389/fimmu.2018.01757

DO - 10.3389/fimmu.2018.01757

M3 - Journal article

C2 - 30108587

VL - 9

JO - Frontiers in Immunology

JF - Frontiers in Immunology

SN - 1664-3224

M1 - 1757

ER -