Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Abstract

Chromatin immunoprecipitation (ChIP) is a powerful technique allowing for investigation of protein-DNA interactions in living cells. Here, we provide a detailed step-by-step protocol for ChIP and highlight important considerations, challenges and pitfalls often encountered in the ChIP procedure. Furthermore, we present data of key quality control (QC) steps and exemplify material performance validation on transcription factor ChIP to provide a QC guide for setting up ChIP. Finally, we provide guidelines for scaling of the ChIP procedure to ChIP sequencing (ChIP-seq) and discuss important considerations associated with this.

Original languageEnglish
Title of host publicationTwo-Hybrid Systems : Methods and Protocols
EditorsLuis Oñate-Sánchez
PublisherHumana Press
Publication date2018
Pages335-352
ISBN (Print)978-1-4939-7870-0
ISBN (Electronic)978-1-4939-7871-7
DOIs
Publication statusPublished - 2018
SeriesMethods in Molecular Biology
Volume1794
ISSN1064-3745

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