Chirality transmission in macromolecular domains

Shankar Pandey, Shankar Mandal, Mathias Bogetoft Danielsen, Asha Brown, Changpeng Hu, Niels Johan Christensen, Alina Vitaliyivna Kulakova, Shixi Song, Tom Brown, Knud J. Jensen, Jesper Wengel, Chenguang Lou*, Hanbin Mao*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Chiral communications exist in secondary structures of foldamers and copolymers via a network of noncovalent interactions within effective intermolecular force (IMF) range. It is not known whether long-range chiral communication exists between macromolecular tertiary structures such as peptide coiled-coils beyond the IMF distance. Harnessing the high sensitivity of single-molecule force spectroscopy, we investigate the chiral interaction between covalently linked DNA duplexes and peptide coiled-coils by evaluating the binding of a diastereomeric pair of three DNA-peptide conjugates. We find that right-handed DNA triple helices well accommodate peptide triple coiled-coils of the same handedness, but not with the left-handed coiled-coil stereoisomers. This chiral communication is effective in a range (<4.5 nm) far beyond canonical IMF distance. Small-angle X-ray scattering and molecular dynamics simulation indicate that the interdomain linkers are tightly packed via hydrophobic interactions, which likely sustains the chirality transmission between DNA and peptide domains. Our findings establish that long-range chiral transmission occurs in tertiary macromolecular domains, explaining the presence of homochiral pairing of superhelices in proteins.

Original languageEnglish
Article number76
JournalNature Communications
Number of pages11
Publication statusPublished - Jan 2022


  • DNA/chemistry
  • Hydrophobic and Hydrophilic Interactions
  • Macromolecular Substances/chemistry
  • Models, Molecular
  • Molecular Docking Simulation
  • Molecular Structure
  • Peptides/chemistry
  • Protein Domains
  • Protein Structure, Secondary
  • Proteins/chemistry
  • Stereoisomerism


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