Chemically modified oligonucleotides with efficient RNase H response

Birte Vester; Anne Marie Boel; Sune Lobedanz; B Ravindra Babu; Michael Raunkjaer; Dorthe Lindegaard; null Raunak; Patrick J Hrdlicka; Torben Højland; Pawan K Sharma; Surender Kumar; Poul Nielsen; Jesper Wengel

doi:10.1016/j.bmcl.2008.03.004

Chemically modified oligonucleotides with efficient RNase H response

Birte Vester, Anne Marie Boel, Sune Lobedanz, B Ravindra Babu, Michael Raunkjaer, Dorthe Lindegaard, Raunak, Patrick J Hrdlicka, Torben Højland, Pawan K Sharma, Surender Kumar, Poul Nielsen, Jesper Wengel

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

Original language	English
Journal	Bioorganic & Medicinal Chemistry Letters
Volume	18
Issue number	7
Pages (from-to)	2296-2300
Number of pages	4
ISSN	0960-894X
DOIs	https://doi.org/10.1016/j.bmcl.2008.03.004
Publication status	Published - 1. Apr 2008

Keywords

Base Sequence
Binding Sites
DNA
Nucleic Acid Conformation
Oligonucleotides
RNA
Ribonuclease H
Substrate Specificity

Documents & Links

10.1016/j.bmcl.2008.03.004

Cite this

@article{f04a50304e5d11ddb1a1000ea68e967b,

title = "Chemically modified oligonucleotides with efficient RNase H response",

abstract = "Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.",

keywords = "Base Sequence, Binding Sites, DNA, Nucleic Acid Conformation, Oligonucleotides, RNA, Ribonuclease H, Substrate Specificity",

author = "Birte Vester and Boel, {Anne Marie} and Sune Lobedanz and Babu, {B Ravindra} and Michael Raunkjaer and Dorthe Lindegaard and Raunak and Hrdlicka, {Patrick J} and Torben H{\o}jland and Sharma, {Pawan K} and Surender Kumar and Poul Nielsen and Jesper Wengel",

year = "2008",

month = apr,

day = "1",

doi = "10.1016/j.bmcl.2008.03.004",

language = "English",

volume = "18",

pages = "2296--2300",

journal = "Bioorganic & Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier",

number = "7",

}

TY - JOUR

T1 - Chemically modified oligonucleotides with efficient RNase H response

AU - Vester, Birte

AU - Boel, Anne Marie

AU - Lobedanz, Sune

AU - Babu, B Ravindra

AU - Raunkjaer, Michael

AU - Lindegaard, Dorthe

AU - Raunak, null

AU - Hrdlicka, Patrick J

AU - Højland, Torben

AU - Sharma, Pawan K

AU - Kumar, Surender

AU - Nielsen, Poul

AU - Wengel, Jesper

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

AB - Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.

KW - Base Sequence

KW - Binding Sites

KW - DNA

KW - Nucleic Acid Conformation

KW - Oligonucleotides

KW - RNA

KW - Ribonuclease H

KW - Substrate Specificity

U2 - 10.1016/j.bmcl.2008.03.004

DO - 10.1016/j.bmcl.2008.03.004

M3 - Journal article

C2 - 18356048

SN - 0960-894X

VL - 18

SP - 2296

EP - 2300

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

IS - 7

ER -

Chemically modified oligonucleotides with efficient RNase H response

Abstract

Keywords

Documents & Links

Fingerprint

Cite this