Characterization of Synthetic Peptides by Mass Spectrometry

Bala K Prabhala, Osman Mirza, Peter Højrup, Paul R Hansen

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Mass spectrometry (MS) is well suited for analysis of the identity and purity of synthetic peptides. The sequence of a synthetic peptide is most often known, so the analysis is mainly used to confirm the identity and purity of the peptide. Here, simple procedures are described for MALDI-TOF-MS and LC-MS of synthetic peptides.

Original languageEnglish
Title of host publicationPeptide Antibodies : Methods and Protocols
EditorsGunnar Houen
Volume1348
PublisherSpringer
Publication date2015
Pages77-82
ISBN (Print)978-1-4939-2998-6
ISBN (Electronic)ISBN978-1-4939-2999-3
DOIs
Publication statusPublished - 2015
SeriesMethods in Molecular Biology
ISSN1064-3745

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Peptides
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Tandem Mass Spectrometry

Cite this

Prabhala, B. K., Mirza, O., Højrup, P., & Hansen, P. R. (2015). Characterization of Synthetic Peptides by Mass Spectrometry. In G. Houen (Ed.), Peptide Antibodies: Methods and Protocols (Vol. 1348, pp. 77-82). Springer. Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-2999-3_9
Prabhala, Bala K ; Mirza, Osman ; Højrup, Peter ; Hansen, Paul R. / Characterization of Synthetic Peptides by Mass Spectrometry. Peptide Antibodies: Methods and Protocols. editor / Gunnar Houen. Vol. 1348 Springer, 2015. pp. 77-82 (Methods in Molecular Biology).
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Prabhala, BK, Mirza, O, Højrup, P & Hansen, PR 2015, Characterization of Synthetic Peptides by Mass Spectrometry. in G Houen (ed.), Peptide Antibodies: Methods and Protocols. vol. 1348, Springer, Methods in Molecular Biology, pp. 77-82. https://doi.org/10.1007/978-1-4939-2999-3_9

Characterization of Synthetic Peptides by Mass Spectrometry. / Prabhala, Bala K; Mirza, Osman; Højrup, Peter; Hansen, Paul R.

Peptide Antibodies: Methods and Protocols. ed. / Gunnar Houen. Vol. 1348 Springer, 2015. p. 77-82 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

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AB - Mass spectrometry (MS) is well suited for analysis of the identity and purity of synthetic peptides. The sequence of a synthetic peptide is most often known, so the analysis is mainly used to confirm the identity and purity of the peptide. Here, simple procedures are described for MALDI-TOF-MS and LC-MS of synthetic peptides.

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Prabhala BK, Mirza O, Højrup P, Hansen PR. Characterization of Synthetic Peptides by Mass Spectrometry. In Houen G, editor, Peptide Antibodies: Methods and Protocols. Vol. 1348. Springer. 2015. p. 77-82. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-2999-3_9