Characterization of native 40 S particles from Krebs II mouse ascites tumor cells: resolution, nomenclature and molecular weights of the nonribosomal proteins

G Reichert, O G Issinger

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Native 40 S particles from Krebs II mouse ascites tumor cells were isolated on a large scale. A nonribosomal protein moiety of about 30 proteins could be removed from the ribosomal particles by treatment with 250 mM KCl. These proteins were analysed by two-dimensional polyacrylamide gel electrophoresis and turned out to be mostly acidic in nature. The molecular weights of about 17 proteins were determined by three-dimensional gel electrophoresis. Radioactively labelled nonribosomal protein spots were excised from two-dimensional gel electrophoresis. Radioactively labelled nonribosomal protein spots were excised from two-dimensional gels and transferred directly or after electrodialysis onto the third dimension gel. The proteins fell into a molecular weight range from about 20,000 to 300,000.
Original languageEnglish
JournalBBA General Subjects
Volume654
Issue number2
Pages (from-to)268-278
Number of pages10
ISSN0304-4165
Publication statusPublished - 1981

Keywords

  • Animals
  • Carcinoma, Krebs 2
  • Electrophoresis, Polyacrylamide Gel
  • Mice
  • Molecular Weight
  • Neoplasm Proteins
  • Peptide Initiation Factors
  • Ribosomes
  • Terminology as Topic

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