We want to identify and quantified phosphoproteins involved in manganese use efficiency, focusing on the phosphoproteome from thylakoid preparations from two barley genotypes, manganese efficient (Vanessa) and inefficient (Antonia) genotype.
Experimental: By monitoring the photosynthetic efficiency (Fv/Fm) a decline in activity is observed as a consequence of manganese deficiency. Barley leaves will be harvested at three specific (Fv/Fm) values after manganese depletion, and again after resupply of manganese. Protein from the purified thylakoids will be digested using a modified spin filter-based protocol that includes trypsin digestion in 0.5 % SDC that enables better protein solubilization. Phosphopeptides will be purified using titaniumdioxide beads. Peptides will be analyzed and label free quantified using a 1D-LC UPLC system coupled to an Orbitrap Velos mass spectrometer.
|Publication date||15. Apr 2013|
|Publication status||Published - 15. Apr 2013|
|Event||phosphoproteomics - University of Southern Denmark, Odense, Denmark|
Duration: 14. Apr 2013 → 19. Apr 2013
|Location||University of Southern Denmark|
|Period||14/04/2013 → 19/04/2013|