Change in autoantibody and cytokine responses during the evolution of neuromyelitis optica in patients with systemic lupus erythematosus

A preliminary study

Katalin T Kovacs, Sudhakar Reddy Kalluri, Antonio Boza-Serrano, Tomas Deierborg, Tunde Csepany, Magdolna Simo, Laszlo Rokusz, Attila Miseta, Nicolas Alcaraz, Laszlo Czirjak, Timea Berki, Tihamer Molnar, Bernhard Hemmer, Zsolt Illes

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

BACKGROUND: Neuromyelitis optica (NMO)-systemic lupus erythematosus (SLE) association is a rare condition characterized by multiple autoantibodies.

OBJECTIVE: To examine if, during the evolution of NMO, anti-AQP4 responses are part of polyclonal B cell activation, and if T cell responses contribute.

METHODS: In 19 samples of six patients who developed NMO during SLE, we examined the correlation of AQP4-IgG1 and IgM with (i) anti-MOG IgG and IgM, (ii) anti-nuclear, anti-nucleosome and anti-dsDNA IgG antibodies, (iii) cytokines and chemokines in the serum and (iv) longitudinal relation to NMO relapses/remission.

RESULTS: AQP4-IgG1 was present 1-2-5 years before the first NMO relapse. During relapse, AQP4-IgG1, ANA, anti-dsDNA and anti-nucleosome antibodies were elevated. Anti-MOG IgG/IgM and AQP4-IgM antibodies were not detected. AQP4-IgG1 antibodies correlated with concentration of anti-nucleosome, IFN-γ,interferon-gamma-induced CCL10/IP-10 and CCL17/TARC (p<0.05, respectively). CCL17/TARC correlated with levels of anti-nucleosome and anti-dsDNA (p<0.05, respectively). Compared to healthy subjects, concentration of IFN-γ and CCL17/TARC was higher in NMO/SLE (p<0.05).

CONCLUSIONS: AQP4-IgG1 antibodies are present in the sera years before the first NMO attack in patients with SLE; elevation of anti-AQP4 is part of a polyclonal B cell response during NMO relapses; in spite of multiple autoantibodies in the serum, MOG antibodies were not present; Th1 responses accompany autoantibody responses in NMO/SLE.

Original languageEnglish
JournalMultiple Sclerosis Journal
Volume22
Issue number9
Pages (from-to)1192-1201
ISSN1352-4585
DOIs
Publication statusPublished - 2016

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Neuromyelitis Optica
Nucleosomes
Serum
Interferon-gamma

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Kovacs, Katalin T ; Kalluri, Sudhakar Reddy ; Boza-Serrano, Antonio ; Deierborg, Tomas ; Csepany, Tunde ; Simo, Magdolna ; Rokusz, Laszlo ; Miseta, Attila ; Alcaraz, Nicolas ; Czirjak, Laszlo ; Berki, Timea ; Molnar, Tihamer ; Hemmer, Bernhard ; Illes, Zsolt. / Change in autoantibody and cytokine responses during the evolution of neuromyelitis optica in patients with systemic lupus erythematosus : A preliminary study. In: Multiple Sclerosis Journal. 2016 ; Vol. 22, No. 9. pp. 1192-1201 .
@article{087ca9cbb0bc44ba8f3e5e629986bdf8,
title = "Change in autoantibody and cytokine responses during the evolution of neuromyelitis optica in patients with systemic lupus erythematosus: A preliminary study",
abstract = "BACKGROUND: Neuromyelitis optica (NMO)-systemic lupus erythematosus (SLE) association is a rare condition characterized by multiple autoantibodies.OBJECTIVE: To examine if, during the evolution of NMO, anti-AQP4 responses are part of polyclonal B cell activation, and if T cell responses contribute.METHODS: In 19 samples of six patients who developed NMO during SLE, we examined the correlation of AQP4-IgG1 and IgM with (i) anti-MOG IgG and IgM, (ii) anti-nuclear, anti-nucleosome and anti-dsDNA IgG antibodies, (iii) cytokines and chemokines in the serum and (iv) longitudinal relation to NMO relapses/remission.RESULTS: AQP4-IgG1 was present 1-2-5 years before the first NMO relapse. During relapse, AQP4-IgG1, ANA, anti-dsDNA and anti-nucleosome antibodies were elevated. Anti-MOG IgG/IgM and AQP4-IgM antibodies were not detected. AQP4-IgG1 antibodies correlated with concentration of anti-nucleosome, IFN-γ,interferon-gamma-induced CCL10/IP-10 and CCL17/TARC (p<0.05, respectively). CCL17/TARC correlated with levels of anti-nucleosome and anti-dsDNA (p<0.05, respectively). Compared to healthy subjects, concentration of IFN-γ and CCL17/TARC was higher in NMO/SLE (p<0.05).CONCLUSIONS: AQP4-IgG1 antibodies are present in the sera years before the first NMO attack in patients with SLE; elevation of anti-AQP4 is part of a polyclonal B cell response during NMO relapses; in spite of multiple autoantibodies in the serum, MOG antibodies were not present; Th1 responses accompany autoantibody responses in NMO/SLE.",
author = "Kovacs, {Katalin T} and Kalluri, {Sudhakar Reddy} and Antonio Boza-Serrano and Tomas Deierborg and Tunde Csepany and Magdolna Simo and Laszlo Rokusz and Attila Miseta and Nicolas Alcaraz and Laszlo Czirjak and Timea Berki and Tihamer Molnar and Bernhard Hemmer and Zsolt Illes",
note = "{\circledC} The Author(s), 2015.",
year = "2016",
doi = "10.1177/1352458515613165",
language = "English",
volume = "22",
pages = "1192--1201",
journal = "Multiple Sclerosis Journal",
issn = "1352-4585",
publisher = "SAGE Publications",
number = "9",

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Kovacs, KT, Kalluri, SR, Boza-Serrano, A, Deierborg, T, Csepany, T, Simo, M, Rokusz, L, Miseta, A, Alcaraz, N, Czirjak, L, Berki, T, Molnar, T, Hemmer, B & Illes, Z 2016, 'Change in autoantibody and cytokine responses during the evolution of neuromyelitis optica in patients with systemic lupus erythematosus: A preliminary study', Multiple Sclerosis Journal, vol. 22, no. 9, pp. 1192-1201 . https://doi.org/10.1177/1352458515613165

Change in autoantibody and cytokine responses during the evolution of neuromyelitis optica in patients with systemic lupus erythematosus : A preliminary study. / Kovacs, Katalin T; Kalluri, Sudhakar Reddy; Boza-Serrano, Antonio; Deierborg, Tomas; Csepany, Tunde; Simo, Magdolna; Rokusz, Laszlo; Miseta, Attila; Alcaraz, Nicolas; Czirjak, Laszlo; Berki, Timea; Molnar, Tihamer; Hemmer, Bernhard; Illes, Zsolt.

In: Multiple Sclerosis Journal, Vol. 22, No. 9, 2016, p. 1192-1201 .

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Change in autoantibody and cytokine responses during the evolution of neuromyelitis optica in patients with systemic lupus erythematosus

T2 - A preliminary study

AU - Kovacs, Katalin T

AU - Kalluri, Sudhakar Reddy

AU - Boza-Serrano, Antonio

AU - Deierborg, Tomas

AU - Csepany, Tunde

AU - Simo, Magdolna

AU - Rokusz, Laszlo

AU - Miseta, Attila

AU - Alcaraz, Nicolas

AU - Czirjak, Laszlo

AU - Berki, Timea

AU - Molnar, Tihamer

AU - Hemmer, Bernhard

AU - Illes, Zsolt

N1 - © The Author(s), 2015.

PY - 2016

Y1 - 2016

N2 - BACKGROUND: Neuromyelitis optica (NMO)-systemic lupus erythematosus (SLE) association is a rare condition characterized by multiple autoantibodies.OBJECTIVE: To examine if, during the evolution of NMO, anti-AQP4 responses are part of polyclonal B cell activation, and if T cell responses contribute.METHODS: In 19 samples of six patients who developed NMO during SLE, we examined the correlation of AQP4-IgG1 and IgM with (i) anti-MOG IgG and IgM, (ii) anti-nuclear, anti-nucleosome and anti-dsDNA IgG antibodies, (iii) cytokines and chemokines in the serum and (iv) longitudinal relation to NMO relapses/remission.RESULTS: AQP4-IgG1 was present 1-2-5 years before the first NMO relapse. During relapse, AQP4-IgG1, ANA, anti-dsDNA and anti-nucleosome antibodies were elevated. Anti-MOG IgG/IgM and AQP4-IgM antibodies were not detected. AQP4-IgG1 antibodies correlated with concentration of anti-nucleosome, IFN-γ,interferon-gamma-induced CCL10/IP-10 and CCL17/TARC (p<0.05, respectively). CCL17/TARC correlated with levels of anti-nucleosome and anti-dsDNA (p<0.05, respectively). Compared to healthy subjects, concentration of IFN-γ and CCL17/TARC was higher in NMO/SLE (p<0.05).CONCLUSIONS: AQP4-IgG1 antibodies are present in the sera years before the first NMO attack in patients with SLE; elevation of anti-AQP4 is part of a polyclonal B cell response during NMO relapses; in spite of multiple autoantibodies in the serum, MOG antibodies were not present; Th1 responses accompany autoantibody responses in NMO/SLE.

AB - BACKGROUND: Neuromyelitis optica (NMO)-systemic lupus erythematosus (SLE) association is a rare condition characterized by multiple autoantibodies.OBJECTIVE: To examine if, during the evolution of NMO, anti-AQP4 responses are part of polyclonal B cell activation, and if T cell responses contribute.METHODS: In 19 samples of six patients who developed NMO during SLE, we examined the correlation of AQP4-IgG1 and IgM with (i) anti-MOG IgG and IgM, (ii) anti-nuclear, anti-nucleosome and anti-dsDNA IgG antibodies, (iii) cytokines and chemokines in the serum and (iv) longitudinal relation to NMO relapses/remission.RESULTS: AQP4-IgG1 was present 1-2-5 years before the first NMO relapse. During relapse, AQP4-IgG1, ANA, anti-dsDNA and anti-nucleosome antibodies were elevated. Anti-MOG IgG/IgM and AQP4-IgM antibodies were not detected. AQP4-IgG1 antibodies correlated with concentration of anti-nucleosome, IFN-γ,interferon-gamma-induced CCL10/IP-10 and CCL17/TARC (p<0.05, respectively). CCL17/TARC correlated with levels of anti-nucleosome and anti-dsDNA (p<0.05, respectively). Compared to healthy subjects, concentration of IFN-γ and CCL17/TARC was higher in NMO/SLE (p<0.05).CONCLUSIONS: AQP4-IgG1 antibodies are present in the sera years before the first NMO attack in patients with SLE; elevation of anti-AQP4 is part of a polyclonal B cell response during NMO relapses; in spite of multiple autoantibodies in the serum, MOG antibodies were not present; Th1 responses accompany autoantibody responses in NMO/SLE.

U2 - 10.1177/1352458515613165

DO - 10.1177/1352458515613165

M3 - Journal article

VL - 22

SP - 1192

EP - 1201

JO - Multiple Sclerosis Journal

JF - Multiple Sclerosis Journal

SN - 1352-4585

IS - 9

ER -