Abstract
The objective of this study was to investigate the suitability of carbohydrate plasma volume expanders as
a novel polymer platform for tumor targeting. Many synthetic polymers have already been synthesized for
targeted tumor therapy, but potential advantages of these carbohydrates include inexpensive synthesis,
constant availability, a good safety profile, biodegradability and the long clinical use as plasma expanders.
Three polymers have been tested for cytotoxicity and cytokine activation in cell cultures and conjugated
with a near-infrared fluorescent dye: hydroxyethyl starches (HES 200 kDa and HES 450 kDa) and dextran
(DEX 500 kDa). Particle size and molecular weight distribution were determined by asymmetric flow
field-flow fractionation (AF4). The biodistribution was investigated non-invasively in nude mice using
multispectral optical imaging. The most promising polymer conjugate was characterized in human colon
carcinoma xenograft bearing nude mice. A tumor specific accumulation of HES 450 was observed, which
proves it’s potential as carrier for passive tumor targeting.
a novel polymer platform for tumor targeting. Many synthetic polymers have already been synthesized for
targeted tumor therapy, but potential advantages of these carbohydrates include inexpensive synthesis,
constant availability, a good safety profile, biodegradability and the long clinical use as plasma expanders.
Three polymers have been tested for cytotoxicity and cytokine activation in cell cultures and conjugated
with a near-infrared fluorescent dye: hydroxyethyl starches (HES 200 kDa and HES 450 kDa) and dextran
(DEX 500 kDa). Particle size and molecular weight distribution were determined by asymmetric flow
field-flow fractionation (AF4). The biodistribution was investigated non-invasively in nude mice using
multispectral optical imaging. The most promising polymer conjugate was characterized in human colon
carcinoma xenograft bearing nude mice. A tumor specific accumulation of HES 450 was observed, which
proves it’s potential as carrier for passive tumor targeting.
| Original language | English |
|---|---|
| Journal | Carbohydrate Polymers |
| Volume | 95 |
| Pages (from-to) | 404-413 |
| Number of pages | 10 |
| ISSN | 0144-8617 |
| DOIs | |
| Publication status | Published - 2013 |
Keywords
- Hydroxyethyl starch
- Multispectral optical imaging
- Tumor accumulation
- Passive tumor targeting
- Cytokine induction
- HES